Investigations on phosphate uptake and polyphosphate metabolism by mycorrhized and nonmycorrhized roots of beech and pine as investigated by in vivo ³¹P-NMR

Abstract

Comparative in vivo ³¹P-NMR studies of mycorrhized and nonmycorrhized roots of Fagus sylvatica and Pinus sylvestris and of the fungus Suillus bovinus in pure culture have produced interesting new data. With respect to intracellular compartments and pH, ³¹P-NMR spectroscopy showed that the spectrum of the mycorrhiza results from simple superimposition of the spectra of its symbionts. A special method of cyclic phosphate supply followed by block averaging of the NMR spectra was used to determine the kinetic behaviour of phosphate uptake and storage and its incorporation into polyphosphate at a constant external pH of 5.5. Mycorrhized roots and pure fungus showed transformation of accumulated inorganic phosphate into mobile polyphosphate with a medium chain length. Transformation of mobile into immobile polyphosphate either with a long chain length or in a granular state was also observed. Thus, two different types of fungal polyphosphate could be verified. Deficiency of external phosphate initiated the mobilization of internal phosphate, transforming stored polyphosphate into phosphate. It could be shown that a high fungal mass renders mycorrhizal phosphate metabolism less sensitive to external variation in nutrient concentration. The central role of the fungus in regulating mycorrhizal phosphate metabolism is discussed.