The degree of lipid fluidity (LFU) was quantitatively monitored by fluorescence polarization analysis of the hydrocarbon fluorescent probe diphenylhexatriene when embedded in artificial and biological lipid complexes. The results have shown a marked increase in LFU in serum lipids associated with the development of spontaneous mammary tumors in C3H mice. An increase in serum LFU was observed during the initiation of primary tumors. The serum LFU further increases as a function of increase in the tumor volume. This increase was not observed when animals were given transplants of syngeneic, spontaneously arising mammary tumors or following implants of antigenically inert glass beads. Since the serum LFU in C57BL/6, NZB and A/JAX non-tumor-bearing mice was found to be significantly lower than in C3H non-tumor-bearing mice, it is suggested that alterations in the dynamics of serum lipids in the C3H system may have a direct relation to the induction and/or growth of spontaneous tumors in these mice. Moreover, experiments carried out with an artificial membrane model system have shown that the dynamics of a lipid complex are directly determined by its lipid composition. The three major parameters that determine the LFU of a lipid domain are: (a) the relative amounts of different phospholipids; (b) the molar ratio of cholesterol to phospholipids; and (c) the degree of saturation of the fatty acids. It is therefore suggested that alterations of this kind may occur in serum lipids during spontaneous mammary tumor development in C3H mice.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
lipid fluidity units
Basu TK, Williams DC (1975) Plasma and body lipids in patients with carcinoma of the breast. Oncology 31:172–176.
Berke G, Tzur R, Inbar M (1978) Changes in fluorescence polarization of a membrane probe during lymphocyte-target cell interaction. J Immunol 120:1478–1484.
Bittner JJ (1939) Influences of breast cancer development in mice. Public Health Rep (Washington DC) 54:1590–1597.
Bittner JJ (1942a) Observations on the genetics of susceptibility for the development of mammary cancer in mice. Cancer Res 2:540–545.
Bittner JJ (1942b) The milk influence of breast tumors in mice. Science 95:462–463.
Chapman HA, Hibbs JB (1977) Modulation of macrophage tumoricidal capability by components of normal serum: A central role for lipid. Science 197:282–285.
Cox RA, Gökcen M (1975) Effect of simian virus 40 subcutaneous tumors on circulating lipids and lipoproteins in the Syrian hamster. J Natl Cancer Inst 54:379–386.
Haven FL, Bloor WR (1956) Lipids in cancer. Adv Cancer Res 4:237–306.
Inbar M, Shinitzky M (1974) Cholesterol as a bioregulator in the development and inhibition of leukemia. Proc Natl Acad Sci USA 71:4229–4231.
Kannan R, Baker N (1977) Hypertriglyceridemia in Ehrlich ascites carcinomatous mice: tumor and mouse strain differences. Lipids 12:153–158.
Kremmer T, Holozinger L (1976) A study of hyperlipidaemia induced by ascites tumours. Acta Morphol Acad Sci Hung 24:369–379.
Narayan KA (1971) Rat serum hypoproteins during carcinogenesis of the liver in the preneoplastic and the neoplastic state. Int J Cancer 8:61–70.
Petitou M, Tuy F, Rosenfeld C, Mishal Z, Paintrand M, Jasmin C, Mathé G, Inbar M (1978) Decreased microviscosity of membrane lipids in leukemic cells: Two possible mechanisms. Proc Natl Acad Sci USA 75:2306–2310.
Rosenfeld C, Jasmin C, Mathé G, Inbar M (1979) Dynamic and composition of cellular membranes and serum lipids in malignant disorders. In: Bonadonna G, Mathé G, Salmon SE (eds) Recent Results in Cancer Research vol 67 Springer, Berlin Heidelberg New York, pp 63–77.
Shinitzky M, Inbar M (1974) Difference in microviscosity induced by different cholesterol levels in the surface membrane lipid layer of normal lymphocytes and malignant lymphoma cells. J Mol Biol 85:603–615.
Shinitzky M, Inbar M (1976) Microviscosity parameters and protein mobility in biological membranes. Biochim Biophys Acta 433:133–149.
Van Blitterswijk WJ, Emmelot P, Hilkmann HAM, Oomenmeulemans EPM, Inbar M (1977) Differences in lipid fluidity among isolated plasma membranes of normal and leukemic lymphocytes and membranes exfoliated from their cell surface. Biochim Biophys Acta 467:309–320.
Recipient of NIH Fellowship (IF32CA 06293-02) awarded by NCI
About this article
Cite this article
Lane, M.A., Mahoney, R.J., Watson, A.L.M. et al. Increased fluidity of serum lipids and development of spontaneous mammary tumors in C3H mice. Cancer Immunol Immunother 8, 49–54 (1980). https://doi.org/10.1007/BF00206354
- Serum Lipid
- Mammary Tumor
- Polarization Analysis
- Fluorescence Polarization
- Lipid Complex