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Lipid-body lipoxygenase is expressed in cotyledons during germination prior to other lipoxygenase forms

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Lipid bodies are degraded during germination. Whereas some proteins, e.g. oleosins, are synthesized during the formation of lipid bodies of maturating seeds, a new set of proteins, including a specific form of lipoxygenase (LOX; EC, is detectable in lipid bodies during the stage of fat degradation in seed germination. In cotyledons of cucumber (Cucumis sativus L.) seedlings at day 4 of germination, the most conspicuous staining with anti-LOX antibodies was observed in the cytosol. At very early stages of germination, however, the LOX form present in large amounts and synthesized preferentially was the lipid-body LOX. This was demonstrated by immunocytochemical staining of cotyledons from 1-h and 24-h-old seedlings: the immunodecoration of sections of 24-h-old seedlings with anti-LOX antiserum showed label exclusively correlated with lipid bodies of around 3 μm in diameter. In accordance, the profile of LOX protein isolated from lipid bodies during various stages of germination showed a maximum at day 1. By measuring biosynthesis of the protein in vivo we demonstrated that the highest rates of synthesis of lipid-body LOX occurred at day 1 of germination. The early and selective appearance of a LOX form associated with lipid bodies at this stage of development is discussed.

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This work was financially supported by the Deutsche Forschungsgemeinschaft, SFB 286 (I.F., A.N., H.K.) and SFB 363 (B.H., C.W.).

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Feussner, I., Hause, B., Nellen, A. et al. Lipid-body lipoxygenase is expressed in cotyledons during germination prior to other lipoxygenase forms. Planta 198, 288–293 (1996).

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Key words

  • Cucumis
  • Germination
  • Lipid body (immunocytochemical staining)
  • Lipoxygenase
  • Protein synthesis (in vivo)