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Expression and regulation of Q8 b in a transfected cell line

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Abstract

RNase protection experiments showed that Q8 b was actively transcribed in a stably transfected cell line. Moreover, Q8 b responded to interferon-γ (IFN-γ) treatment with increased levels of mRNA expression. Thus Q8 b demonstrates a regulatory response to IFN-γ characteristic of many other class I genes. Cell surface expression of a Q8b product could also be detected by flow cytometric analysis with the Qa-2-specific monoclonal antibody D3.262. The expression of the Q8b cell surface product increased only slightly after cells were treated with IFN-γ. The Q8b cell surface product was not sensitive to cleavage by phosphatidylinositol-phospholipase C. These results suggest that the Q8b product, unlike the predominant forms of Qa-2-bearing molecules, is not anchored via phosphatidylinositol to the cell membrane. These results also suggest that Q8 b has the potential to contribute to the Qa-2 phenotype in vivo.

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Address correspondence and offprint requests to: L. Flaherty.

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Waters, J.B., Flaherty, L. Expression and regulation of Q8 b in a transfected cell line. Immunogenetics 34, 179–184 (1991). https://doi.org/10.1007/BF00205821

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Keywords

  • Cell Membrane
  • Monoclonal Antibody
  • mRNA Expression
  • Cell Surface
  • Surface Expression