Expression of HLA-DR antigens by purified preparations of human ovarian carcinoma cells freshly isolated from surgical specimens was examined in parallel with the capacity of tumor cells to elicit a blastogenic response from autologous lymphocytes in mixed lymphocyte-tumor culture (MLTC) assay. Of 21 tumor preparations, 11 (52%) reacted with monoclonal antibodies 279 and/or 949 specific for a monomorphic determinant of HLA-DR antigens, with heterogeneous positivity, ranging between 30% and 95%. In this series of patients positive MLTC occurred in 8/21 individual experiments. The HLA-DR expression was proportionally similar in tumors giving positive MLTC (4/8=50%) and negative MLTC (7/13=53%). The lack of correlation between DR expression on tumor cells and stimulatory activity in autologous MLTC and the fact that DR-negative tumors could induce lymphocyte stimulation, support the hypothesis that blastogenesis occurs upon recognition of tumor-associated antigens, different from DR molecules, possibly tumor-specific antigens.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Allavena P, Introna M, Sessa C, Mangioni C, Mantovani A (1982) Interferon effect on cytotoxicity of peripheral blood and tumor-associated lymphocytes against human ovarian carcinoma cells. J Natl Cancer Inst 68:555
Allavena P, Zanaboni F, Rossini S, Merendino A, Bonazzi C, Vassena L, Mangioni C, Mantovani A (1986) Lymphokine-activated killer activity of tumor-associated and peripheral blood lymphocytes isolated from patients with ascites ovarian tumors. J Natl Cancer Inst 77:863
Allen CA, Hogg N (1987) Association of colorectal tumor epithelium expressing HLA-D/DR with CD8-positive T-cells and mononuclear phagocytes. Cancer Res 47:2919
Fossati F, Taramelli D, Balsari A, Bogsanovich G, Andreola S, Parmiani G (1984) Primary but not metastatic human melanomas expressing DR antigens stimulate autologous lymphocytes. Int J Cancer 33:591
Grimm EA, Vose BM, Chu EW, Wilson DJ, Lotze MT, Ravner AA, Rosenberg SA (1984) The human mixed lymphocytetumor cell interaction test. I. Positive autologous lymphocyte proliferative responses can be stimulated by tumor cells as well as by cells from normal tissues. Cancer Immunol Immunother 17:83
Howe AJ, Seeger RC, Molinaro GA, Ferrone S (1981) Analysis of human tumor cells for Ia-like antigens with monoclonal antibodies. J Natl Cancer Inst 66:827
Huber C, Fink W, Leibold W, Schmalzl F, Peterson PA, Klareskog L, Braunsteiner H (1981) The role of adherent HLA-DR+ mononuclear cells in autologous and allogeneic MLR1. J Immunol 127:726
Kabawat SE, Bost RC Jr, Welch WR, Knapp RC, Bhan AK (1983) Expression of major histocompatibility antigens and nature of inflammatory cellular infiltrate in ovarian neoplasms. In J Cancer 32:547
Marty J, Kjeldsberg CR, Groo S (1982) Improved immunoperoxidase stain on frozen sections: An avidin-biotin-peroxidase complex (ABC) technique. Histotechnol 5:61
Nadler LM, Stashenko P, Hardy R, Pesando JM, Yunis EJ, Schlossman SF (1981) Monoclonal antibodies defining serologically distinct HLA-D/DR related Ia-like antigens in man. Hum Immunol 2:77
Natali PG, Demartino C, Quaranto V, Bigotti A, Pellegrino MA, Ferrone S (1981) Changes in Ia-like antigen expression on malignant human cells. Innunogenetics 12:409
Peri G, Polentarutti N, Sessa C, Mangioni C, Mantovani A (1981) Tumoricidal activity of macrophages isolated from human ascitic and solid ovarian carcinomas: Augmentation by interferon, lymphokines and endotoxin. Int J Cancer 28:143
Taylor GM, Ridway JC, Fergusson WD, Harris R (1984) Lack of correlation between lymphocyte activating determinants and HLA-DR on acute leukaemias. Br J Cancer 49:485
Thompson JJ, Herlyn MF, Elder DE, Clark WH, Steplewski Z, Koprowski H (1982) Expression of DR antigens in freshly frozen human tumors. Hybridoma 1:161
Todd RF III, Meuer SC, Romain PL, Schlossman SF (1984) A monoclonal antibody that blocks class II histocompatibility-related immune interactions. Hum Immunol 10:23
Vanky F, Klein E, Stjernsward J, Rodriguez L, Peterffy A, Steiner L, Nilsonne U (1978) Human tumor-lymphocyte interaction in vitro. III. T lymphocytes in autologous tumor stimulation (ATS). Int J Cancer 22:679
Vanky F, Klein E, Willems J (1985) DR antigens expressed on tumor cells do not contribute to the blastogenetic response of autologous T cells. Cancer Immunol Immunother 19:219
Vose BM, Bonnard GD (1982) Human tumour antigens defined by cytotoxicity and proliferative responses of cultured lymphoid cells. Nature 296:359
Vose BM, White W (1983) Tumour-reactive lymphocytes stimulated in mixed lymphocyte and tumour culture. Clonal analysis of effector cells in cytotoxic and proliferative assays. Cancer Immunol Immunother 15:227
About this article
Cite this article
Di Bello, M., Lucchini, V., Chiari, S. et al. DR antigen expression on ovarian carcinoma cells does not correlate with their capacity to elicit an autologous proliferative response. Cancer Immunol Immunother 27, 63–68 (1988). https://doi.org/10.1007/BF00205760
- Tumor Cell
- Ovarian Carcinoma
- Individual Experiment
- Proliferative Response
- Antigen Expression