Use of the multicellular tumor spheroid as a tumor model allows separate host or tumor treatment with ionizing radiation and examination of the effects on host-tumor immune interactions. Spheroids of EMT6/Ro, a BALB/c mammary tumor were implanted into the peritoneal cavity of syngeneic immunized mice, recovered, and dissociated into single cells. Cytolytic activity of mature spheroid associated cells and peritoneal cells was resistant to radiation doses as high as 1000 rads when irradiated directly prior to assay. Mice irradiated (200, 400, 700 rads) 24 h prior to spheroid injection had an increased number of tumor cells and decreased number of tumor infiltrating and peritoneal host cells upon spheroid recovery. This was paralleled by an increased colony forming efficiency per spheroid. Cytolytic activity of the spheroid associated cells against radiolabeled EMT6 cells was in many cases decreased with radiation although lysis was the same on a per cell basis. Cytolytic activity by peritoneal cells from these mice increased with dose as measured on a per cell basis. This activity from irradiated animals was carried out by a Thyl+ cell.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Anderson RE, Standefer JC (1983) Radiation injury of the immune system: effects on cell lineages. In: Potten CS, Hendry JH (eds) Cytotoxic insult to tissue. Churchill Livingstone, Edinburgh, p 67
Brunner KT, Engers HD, Cerottini JC (1976) The 51Cr release assay as used for the quantitative measurement of cell-mediated cytolysis in vitro. In: Bloom BR, David JR (eds) In vitro methods in cell-mediated and tumor immunity. Academic Press, New York, p 423
Conard RA (1969) Quantitative study of radiation effects in phytohaemagglutinin-stimulated leukocyte cultures. Int J Radiat Biol 16:157
Doria G, Agarossi G, Adorini L (1982) Selective effects of ionizing radiations on immunoregulatory cells. Immunol Rev 65:23
Ettinghausen SE, Lipford EH, Mule JJ, Rosenberg SA (1985) Systemic administration of recombinant interleukin 2 stimulates in vivo lymphoid proliferation in tissues. J Immunol 135:1488
Knox SJ, Shifrine M, Rosenblatt L (1982) Assessment of the in vitro radiosensitivity of human peripheral blood lymphocytes. Radiat Res 89:575
Lavin MF, Kidson C (1977) Repair of ionizing radiation induced DNA damage in human lymphocytes. Nucleic Acids Res 4:4015
Lord EM (1980) Comparison of in situ and peripheral host immunity to syngeneic tumours employing the multicellular spheroid model. Br J Cancer 41:123
Lord EM, Burkhardt G (1984) Assessment of in situ immunity to syngeneic tumors utilizing the multicellular spheroid model. Cell Immunol 85:340
Lord EM, Keng PC (1984) Methods for using centrifugal elutriation to separate malignant and lymphoid cell populations. J Immunol Methods 68:147
Lord EM, Nardella G (1980) The multicellular tumor spheroid model II. Characterization of the primary allograft response in unsensitized mice. Transplantation 29:119
Lord EM, Penney DP, Sutherland RM, Cooper RA (1979) Morphological and functional characteristics of cells infiltrating and destroying tumor multicellular spheroids in vivo. Virchows Arch (Cell Pathol) 31:103
Lord EM, Nardella CB, Sutherland RM (1980) The multicellular tumor spheroid model I. Characterization of the allograft response in sensitized mice. Cell Immunol 52:119
Lowenthal JW, Harris AW (1985) Activation of mouse lymphocytes inhibits induction of rapid cell death by X-irradiation. J Immunol 135:1119
MacDonald HR (1982) Differentiation of cytolytic T lymphocytes. Immunol Today 3:183
MacDonald HR, Sordat B (1980) The multicellular tumor spheroid: A quantitative model for studies of in situ immunity. Contemp Top Immunobiol 10:317
Marshak-Rothstein A, Fink P, Gridley T, Raulet DH, Gevan MJ, Gefter ML (1969) Properties and applications of monoclonal antibodies directed against determinants of the Thy-1 locus. J Immunol 122:2491
Mule JJ, Shu S, Rosenberg SA (1985) The anti-tumor efficacy of lymphokine-activated killer cells and recombinant interleukin 2 in vivo. J Immunol 135:646
North RJ (1984) Gamma-irradiation facilitates the expression of adoptive immunity against established tumors by eliminating suppressor T cells. Cancer Immunol Immunother 16:175
North RJ (1984) The therapeutic significance of concomitant antitumor immunity I. Ly1–2+ T cells from mice with a progressive tumor can cause regression of an established tumor in gamma-irradiated recipients. Cancer Immunol Immunother 18:69
Rockwell SC, Kallman RF, Fajardo LF (1972) Characteristics of a serially transplanted mouse mammary tumor and its tissue culture adapted derivative. J Natl Cancer Inst 49:735
Sordat B, MacDonald HR, Lees RK (1980) The multicellular spheroid as a model tumor allograft III. Morphological and kinetic analysis of spheroid infiltration and destruction. Transplantation 29:103
Sutherland RM, Durand RE (1984) Growth and cellular characteristics of multicell spheroids. In Acker H, Carlsson J, Durand R, Sutherland RM (eds) Recent results in cancer research: spheroids in cancer research. Springer-Verlag, Berlin, p 24
Sutherland RM, McCredie JA, Inch WR (1971) Growth of multicell spheroids in tissue culture as a model of nodular carcinomas. J Natl Cancer Inst 46:113
Taupier MA, Reynolds SD, Lord EM (1985) Culture of tumor cells in serum free low protein media allows detection of antigens previously masked. Fed Proc 44:961
About this article
Cite this article
Wilson, K.M., Lord, E.M. Effects of radiation on host-tumor interactions using the multicellular tumor spheroid model. Cancer Immunol Immunother 23, 20–24 (1986). https://doi.org/10.1007/BF00205550
- Cell Basis
- Peritoneal Cavity
- Tumor Model
- Mammary Tumor