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Localization of the gene for human proliferating nuclear antigen/cyclin by in situ hybridization

Summary

Proliferating cell nuclear antigen (PCNA)/ cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. In addition, there were two strong secondary peaks of grains over 11p15.1 and Xp11.4 indicating the presence of two related genes in man.

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References

  1. Almendral JM, Huebsch D, Blundell PA, MacDonald-Bravo H, Bravo R (1987) Cloning and sequence of the human nuclear protein cyclin: homology with DNA-binding proteins. Proc Natl Acad Sci USA 84:1575–1579

  2. Benedum UM, Lamouroux A, Konecki DS, Rosa P, Hille A, Baeuerle PA, Frank R, Lottspeich F, Mallet J, Huttner WB (1987) The primary structure of human secretogranin I (chromogranin B) in comparison with chromogranin A reveals homologous terminal domains and a large intervening variable region. EMBO J 6:1203–1211

  3. Bravo R, MacDonald-Bravo H (1985) Changes in the nuclear distribution of cyclin (PCNA) but not its synthesis depend on DNA replication. EMBO J 4:655–661

  4. Bravo R, Frank R, Blundell PA, MacDonald-Bravo H (1987) Cyclin/PCNA is the auxillary protein of DNA polymerase-δ. Nature 326:515–517

  5. Brissenden JE, Caras I, Thelander L, Francke U (1988) The structural gene for the M1 subunit of ribonucleotide reductase maps to chromosome 11, band p15, in human and to chromosome 7 in mouse. Exp Cell Res 174:302–308

  6. Buckle VJ, Craig IW (1986) In situ hybridization. In: Davies KE (ed) Human genetic diseases — a practical approach. IRL Press, Oxford Washington. pp 85–100

  7. Celis JE, Madsen P (1986) Increased nuclear cyclin/PCNA antigen staining of non S-phase transformed human amnion cells engaged in nucleotide excision DNA repair. FEBS Lett 209:277–283

  8. Donlon TA (1986) Practical approaches to in situ hybridization. Karyogram 12:3–10

  9. Fearon ER, Antonarakis SE, Meyers DA, Levine MA (1984) cHa-ras-1 oncogene lies between β-globin and insulin loci on human chromosome 11p. Am J Hum Genet 36:329–337

  10. Galand P, Dagraef C (1989) Cylin/PCNA immunostaining as an alternative to tritiated thymidine pulse labelling for marking S phase cells in paraffin sections from animal and human tissues. Cell Tissue Kinet 22:383–392

  11. ISCN (1985) An international system for human cytogenetic nomenclature. Harnden DG, Klinger HP (eds) Published in collaboration with Cytogenet Cell Genet. Karger, Basel

  12. Kittur SD, Hoppener JWM, Antonarakis SE, Daniels JDJ, Meyers DA, Maestri NE, Jansen M, Korneluk RG, Nelkin BD, Kazazian HH (1985) Linkage map of the short arm of chromosome 11; location of the genes for catalase, calcitonin and insulin-like growth factor II. Proc Natl Acad Sci USA 82:5064–5067

  13. Kretschmar HA, Stowring LE, Westaway D, Stubblebine WH, Prusiner SB, Dearmond SJD (1986) Molecular cloning of a human prion protein. DNA 5:315–324

  14. Ku D-H, Travali S, Calabretta B, Huebner K, Baserga R (1989) Human gene for proliferating cell nuclear antigen has pseudogenes and localizes to chromosome 20. Somat Cell Mol Genet 15:2297–2307

  15. Litt M, Buroker ME, Kondoleon S, Douglass J, Liston D, Sheehy R, Magenis RE (1988) Chromosomal localization of the human proenkephalin and prodynorphin aenes. Am J Hum Genet 42:327–334

  16. Matsumoto K, Moriuchi T, Koji T, Nakane P (1987) Molecular cloning of cDNA for rat proliferating cell nuclear antigen (PCNA)/cyclin. EMBO J 6:637–642

  17. Miyachi K, Fritzler ML, Tan EM (1978) Autoantibodies to a nuclear antigen in proliferating cells. J Immunol 121:2228–2234

  18. Pardue ML (1985) In situ hybridization. In: Hames BD, Higgins SJ (eds) Nucleic acid hybridization — a practical approach. IRL Press, Oxford Washington. pp 1179–1202

  19. Prelich G, Tan C-K, Kostura M, Mathews MB, So AG, Downey KM, Stillman B (1987) Functional identity of proliferating cell and nuclear antigen and a DNA polymerase-δ auxiliary protein. Nature 326:517–519

  20. Schnittger S, Hofers C, Heidemann P, Beerman F, Hansmann I (1989) Molecular and cytogenetic analysis of an interstitial 20p deletion associated with syndromic intrahepatic ductular hypoplasia (Alagille syndrome). Hum Genet 83:239–244

  21. Zuber M, Tan EM, Ryoji M (1989) Involvement of proliferating cell nuclear antigen (cyclin) in DNA replication in living cells. Mol Cell Biol 9:57–66

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Correspondence to Graham Webb.

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Webb, G., Parsons, P. & Chenevix-Trench, G. Localization of the gene for human proliferating nuclear antigen/cyclin by in situ hybridization. Hum Genet 86, 84–86 (1990). https://doi.org/10.1007/BF00205180

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Keywords

  • Internal Medicine
  • Related Gene
  • Metabolic Disease
  • Proliferate Cell Nuclear Antigen
  • Human Chromosome