Different modes of conditioning of a crude lipase from Rhizomucor miehei were investigated in terms of activity for synthesis of butyl butyrate either by esterification or transesterification in organic media. It was found that lyophilisation alone had a positive effect on reaction rates and that this effect was greatly enhanced when incorporating the substrates of the reaction prior to lyophilisation.These improvements were no longer observed when adding water to the reaction medium. Removal of the insoluble matter from the crude enzyme had a favourable effect on activity only in biphasic water-organic solvent media.
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Correspondence to: F. Monot
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Monot, F., Paccard, E., Borzeix, F. et al. Effect of lipase conditioning on its activity in organic media. Appl Microbiol Biotechnol 39, 483–486 (1993). https://doi.org/10.1007/BF00205037