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Quantitative and qualitative analysis of DNA extracted from postmortem muscle tissues

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Summary

DNA extracted from 33 postmortem muscle specimens was analyzed using MZ 1.3, a hypervariable minisatellite probe, as well as locus-specific minisatellite probes (g3, MS1 and MS43). After storage at −25°C for 10 months, DNA from all the samples was partially (approximately 21% of total DNA) degraded even when autopsy was performed 1 day post mortem. However, more than 90% of DNA samples up to at least 3 days post mortem were suitable to obtain good restriction fragment length polymorphism (RFLP) patterns. When small strips of specimen were stored for 8 days at room temperature in moist chambers, approximately 42% of total DNA was degraded. Only 30% of these DNA samples still showed good RFLP patterns. However, no obvious relation between qualities of DNA analyzed by detection of RFLP and quantities of total and high-MW DNA became apparent. A case of familial relationship was ascertained by DNA fingerprints. Since DNA of good quality can be recovered from muscle tissues in large quantities, DNA extraction from muscle tissues and detection of RFLP patterns should be very useful for individual identification in autopsy cases.

Zusammenfassung

Genomische DNA wurde aus 33 Muskelgewebsproben postmortal extrahiert and im Southern Blot-Verfahren sowohl mit der Multi-locus-Minisatelliten-DNA-Sonde MZ 1.3 als auch mit Locus-spezifischen DNA-Sonden (g3, MS1 and MS43) analysiert. Nach Lagerung der Gewebsproben bei −25°C fur 10 Monate war die DNA von allen Proben partiell degradiert (ca. 25% Anteil degradierter DNA an Gesamt-DNA), auch wenn die Obduktion innerhalb eines Tages nach Eintritt des Totes erfolgte. Dennoch waren über 90% der DNA-Proben, die bis zu drei Tage nach Tod entnommen werden waren, für eine Southern Blot-Analyse geeignet. Bei Lagerversuchen von kleinen Muskelgewebsproben bei Raumtemperatur in einer feuchten Kammer fur acht Tage waren ca. 42% der Gesamt-DNA degradiert. Nur von 30% dieser gelagerten DNA-Proben waren noch Minisatelliten-DNA-Fragmente darzustellen. Es war jedoch keine deutliche Abhängigkeit zwischen dem jeweiligen Anteil von hochmolekularer DNA zu Gesamt-DNA einer Probe and der Detektion von Minisatelliten-DNA-Fragmenten zu erkennen. Zusätzlich konnte in einem Fall eine Verwandtschaftsbeziehung anhand der Minisatelliten-DNA-Fragmente mit MZ 1.3 untersucht werden. Da insgesamt genomische DNA in guter Qualität und ausreichender Menge aus Muskelgewebsproben isoliert werden konnte, erscheint diese Methode zur Darstellung von individuellen DNA-Fragmentmustern bei forensischen Fragestellungen in Autopsiefällen sehr nützlich.

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Correspondence to C. Rittner.

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Ogata, M., Mattern, R., Schneider, P.M. et al. Quantitative and qualitative analysis of DNA extracted from postmortem muscle tissues. Z Rechtsmed 103, 397–406 (1990). https://doi.org/10.1007/BF00204704

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Key words

  • DNA fingerprinting
  • Locus-specific probes
  • DNA stability
  • Muscle tissues
  • Individual identification

Schlüsselwörter

  • Genetischer Fingerabdruck
  • Locus-spezifische DNA-Sonden
  • DNA-Stabilität
  • Muskelgewebe
  • Identifizierung