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Gibberellins and phytochrome regulation of stem elongation in pea

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Abstract

In garden pea (Pisum sativum L.) neither etiolation nor the phytochrome B (phyB)-response mutation lv substantially alters the level of the major active endogenous gibberellin, GA1 in the apical portion of young seedlings. The phyB-controlled responses to continuous red light and end-of-day far-red light are retained even in a GA-overproducing mutant (sln). Comparison of the effects of the lv mutation and GA1 application on seedling development shows important differences in rate of node development, cell extension and division, and leaf development. These results suggest that in pea the control of stem elongation by light in general and phyB in particular is not mediated by changes in GA1 content. Instead, the increased elongation of dark-grown and lv plants appears to result from increased responsiveness of the plant to its endogenous levels of GA1. Three GA1-deficient mutants, na, ls and le have been used to investigate these changes in responsiveness, and study of these and the double mutants na lv, ls lv and le lv has demonstrated that the relative magnitude of the change in responsiveness is dependent on GA1 level. The difference in pleiotropic effects of GA1 application and the lv mutation suggest that light and GA1 interact late in their respective transduction pathways. A model for the relationship between light, GA1 level and elongation in pea is presented and discussed.

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Abbreviations

B:

blue light

cv:

cultivar

EOD-FR:

end-of-day far-red light

FR:

far-red light

GAn :

Gibberellin An

GC-SIM:

gas chromatography-selected ion monitoring

HIR:

high irradiance response

W:

white light

References

  1. Beall, F.D., Morgan, P.W., Mander, L.N., Miller, F.R., Babb, K.H. (1991) Genetic regulation of development in Sorghum bicolor. V. The ma 3 R allele results in gibberellin enrichment. Plant Physiol. 95, 116–125

  2. Behringer, F.J., Cosgrove, D.J., Reid, J.B., Davies, P.J. (1990) Physical basis for altered stem elongation rates in internode length mutants of Pisum. Plant Physiol. 94, 166–173

  3. Campell, B.R., Bonner, B.A. (1986) Evidence for phytochrome regulation of gibberellin A20 3 β-hydroxylation in shoots of dwarf (lele) Pisum sativum L. Plant Physiol. 82, 909–915

  4. Childs, K.L., Pratt, L.H., Morgan, P.W. (1991) Genetic regulation of development in Sorghum bicolor. VI. The ma 3 R allele results in abnormal phytochrome physiology. Plant Physiol. 97, 714–719

  5. Chory, J. (1993) Out of darkness: mutants reveal pathways controlling light-regulated development in plants. Trends Genet. 9, 167–172

  6. Devlin, P.F., Rood, S.B., Somers, D.E., Quail, P.H., Whitelam, G.C. (1992) Photophysiology of the elongated internode (ein) mutant of Brassica rapa. The ein mutant lacks a detectable phytochrome B-like polypeptide. Plant Physiol. 100, 1442–1447

  7. Fang, N., Bonner, B.A., Rappaport, L. (1991) Phytochrome mediation of gibberellin metabolism and epicotyl elongation in cow-pea, Vigna sinensis L. In: Gibberellins, pp. 280–288, Takahashi, N., Phinney, B.O., MacMillan, J., eds., Springer Verlag, New York

  8. Inskeep, W.P., Bloom, P.R. (1985) Extinction coefficients of chlorophyll a and b in N,N-dimethylformamide and 80% acetone. Plant Physiol. 77, 483–485

  9. Jones, A.M., Cochran, D.S., Lamerson, P.M., Evans, M.L., Cohen, J.D. (1991) Red light-regulated growth. I. Changes in the abundance of indoleacetic acid and a 22-kilodalton auxin binding protein in the maize mesocotyl. Plant Physiol. 97, 352–358

  10. Kamiya, Y., Kobayashi, M., Fujioka, S., Yamane, H., Nakayama, I., Sakurai, A. (1991) Effects of a plant growth regulator, prohexadione-calcium (BX-112) on the elongation of rice shoots caused by exogenously applied gibberellins and helminthosporol, part II. Plant Cell Physiol. 32, 1205–1210

  11. Kende, H., Lang, A. (1964) Gibberellins and light inhibition of stem growth in peas. Plant Physiol. 39, 435–440

  12. Kigel, J., Cosgrove, D.J. (1991) Photoinhibition of stem elongation by blue and red light. Effects on hydraulic and cell wall properties. Plant Physiol. 95, 1049–1056

  13. Lopez-Juez, E., Buurmeijer, W.F., Heeringa, G.H., Kendrick, R.E., Wesselius, J.C. (1990) Response of light-grown wild-type and long-hypocotyl mutant cucumber plants to end-of-day far-red light. Photochem. Photobiol. 52, 143–149

  14. Lopez-Juez, E., Kobayashi, M., Kamiya, Y., Kendrick, R.E. (1993) A possible involvement of gibberellins in the action of phytochrome B: a study using the cucumber lh mutant. (Abstr.) Plant Cell Physiol. 34, Suppl., 71

  15. Martínez-García, J.F., García-Martínez, J.L. (1992a) Interaction of gibberellins and phytochrome in the control of cowpea epicotyl elongation. Physiol. Plant. 86, 236–244

  16. Martínez-García, J.F., García-Martínez, J.L. (1992b) Phytochrome modulation of gibberellin metabolism in cowpea epicotyls. In: Progress in plant growth regulation, pp. 585–590, Karssen, C.M., van Loon, L.C., Vreugdenhil, D. eds., Kluwer

  17. Nagatani, A., Reid, J.B., Ross, J.J., Dunnewijk, A., Furuya, M. (1990) Internode length in Pisum. The response to light quality, and phytochrome type I and II levels in lv plants. J. Plant Physiol. 135, 667–674

  18. Nagatani, A., Chory, J., Furuya, M. (1991) Phytochrome B is not detectable in the hy3 mutant of Arabidopsis, which is deficient in responding to end-of-day far-red light treatments. Plant Cell Physiol. 32, 1119–1122

  19. Nakayama, I., Kobayashi, M., Kamiya, Y., Abe, H., Sakurai, A. (1992) Effects of a plant-growth regulator, prohexadione-calcium (BX-112), on the endogenous levels of gibberellins in rice. Plant Cell Physiol. 33, 59–62

  20. Nick, P., Furuya, M. (1993) Phytochrome dependent decrease of gibberellin sensitivity. A case study of cell extension growth in the mesocotyl of japonica and indica type rice cultivars. Plant Growth Regul. 12, 195–206

  21. Noguchi, H., Hashimoto, T. (1990) Phytochrome-mediated synthesis of novel growth inhibitors, A-2α and β, and dwarfism in peas. Planta 181, 256–262

  22. Parks, B.M., Quail, P.H. (1993) hy8, a new class of Arabidopsis long hypocotyl mutants deficient in functional phytochrome A. Plant Cell 5, 39–48

  23. Reid, J.B. (1983) Internode length in Pisum. Do the internode length genes effect growth in dark-grown plants? Plant Physiol. 72, 759–763

  24. Reid, J.B. (1988) Internode length in Pisum. Comparison of genotypes in the light and dark. Physiol. Plant. 74, 83–88

  25. Reid, J.B., Dalton, P.J., Murfet, I.C. (1977) Flowering in Pisum: does gibberellic acid directly influence the flowering process? Aust. J. Plant Physiol. 4, 479–483

  26. Reid, J.B., Ross, J.J. (1988) A new gene, lv, conferring an enhanced response to gibberellin A1. Physiol. Plant. 72, 595–604

  27. Reid, J.B., Murfet, I.C., Potts, W.C. (1983) Internode length in Pisum. II. Additional information on the relationship and action of loci Le, La, Cry, Na and Lm. J. Exp. Bot. 34, 349–364

  28. Reid, J.B., Hasan, O., Ross, J.J. (1990) Internode length in Pisum. Gibberellins and the response to far-red-rich light. J. Plant. Physiol. 137, 46–52

  29. Reid, J.B., Ross, J.J., Swain, S.M. (1992) Internode length in Pisum. A new, slender mutant with elevated levels of C19 gibberellins. Planta 188, 462–467

  30. Rood, S.B., Williams, P.H., Pearce, D., Murofushi, N., Mander, L.N., Pharis, R.P. (1990a). A mutant gene that increases gibberellin production in Brassica. Plant Physiol. 93, 1168–1174

  31. Rood, S.B., Zanewich, K.P., Bray, D.F. (1990b) Growth and development of Brassica genotypes differing in endogenous gibberellin content. II. Gibberellin content, growth analyses and cell size. Physiol. Plant. 79, 679–685

  32. Rood, S.B., Zanewich, K.P., Pearce, D.W. (1993) Photocontrol of gibberellin metabolism in Brassica. (Abstr.) Plant Physiol. 102, Suppl., 8

  33. Ross, J.J., Reid, J.B. (1989) Internode length in Pisum. Biochemical expression of the le gene in darkness. Physiol. Plant. 76, 164–172

  34. Ross, J.J., Willis, C.L., Gaskin, P., Reid, J.B. (1992) Shoot elongation in Lathyrus odoratus L.: gibberellin levels in light- and dark-grown tall and dwarf seedlings. Planta 187, 10–13

  35. Ross, J.J., Reid, J.B., Swain, S.M. (1993a) Control of stem elongation by gibberellin A1: evidence from genetic studies including the slender mutant sln. Aust. J. Plant Physiol. 20, 585–599

  36. Ross, J.J., Murfet, I.C., Reid, J.B. (1993b) Distribution of gibberellins in Lathyrus odoratus L. and their role in leaf growth. Plant Physiol. 102, 603–608

  37. Smith, V.A., Knatt, C.J., Gaskin, P., Reid, J.B. (1992) The distribution of gibberellins in vegetative tissues of Pisum sativum L. I. Biological and biochemical consequences of the le mutation. Plant Physiol. 99, 368–371

  38. Sponsel, V.M. (1983) The localization, metabolism and biological activity of gibberellins in maturing and germinating seeds of Pisum sativum cv. Progress No. 9. Planta 159, 454–468

  39. Sponsel, V.M. (1986) Gibberellins in dark- and red-light-grown shoots of dwarf and tall cultivars of Pisum sativum: the quantification, metabolism and biological activity of gibberellins in Progress No. 9 and Alaska. Planta 168, 119–129

  40. Swain, S.M. (1993) Gibberellins and seed development in Pisum. PhD thesis, University of Tasmania, Hobart, Australia

  41. Talon, M., Zeevaart, J.A.D. (1990) Gibberellins and stem growth as related to photoperiod in Silene armeria L. Plant Physiol. 92, 1094–1100

  42. Toyomasu, T., Yamane, H., Yamaguchi, I., Murofushi, N., Takahashi, N., Inoue, Y. (1992) Control by light of hypocotyl elongation and levels of endogenous gibberellins in seedlings of Lactuca sativa L. Plant Cell Physiol. 33, 695–701

  43. Weller, J.L., Reid, J.B. (1993) Photoperiodism and photocontrol of stem elongation in two photomorphogenic mutants of Pisum sativum L. Planta 189, 15–23

  44. Whitelam, G.C., Smith, H. (1991) Retention of phytochrome-mediated shade avoidance responses in phytochrome-deficient mutants of Arabidopsis, cucumber and tomato. J. Plant Physiol. 139, 119–125

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Additional information

We thank Prof. L.N. Mander for provision of deuterated internal standards, Peter Bobbi, Noel Davies, Omar Hasan, and Katherine McPherson for technical assistance, Stephen Swain for discussion and provision of GA-level data, and the Australian Research Council for financial assistance. J.L.W. is in receipt of an Australian Postgraduate Research scholarship.

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Weller, J.L., Ross, J.J. & Reid, J.B. Gibberellins and phytochrome regulation of stem elongation in pea. Planta 192, 489–496 (1994). https://doi.org/10.1007/BF00203586

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Key words

  • Gibberellin (levels, response)
  • Light (phytochrome B)
  • Mutant (photomorphogenic, gibberellin)
  • Pisum (mutants)
  • Stem elongation