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Gm typing by enzyme-linked immunosorbent assay (ELISA)

Summary

A solid-phase ELISA for Gm typing is described. A mixture of anti-Gm serum (or monoclonal anti-Gm antibody) and test serum was incubated in microtiter wells coated with IgG or its fragments of appropriate Gm type. After washing of the wells, the bound antibody was detected with peroxidase-labeled second antibody. The Glm(3), G3m(16), and G3m(21) antigens could be identified by this technique. Since some of the human anti-Gm sera and anti-Rh0 sera required for the conventional hemagglutination-inhibition method are hard to obtain, the ELISA system using anti-Gm antibodies and no anti-Rh0 sera may serve as an alternative to the conventional method.

Zusammenfassung

Gm-Typisierung wurde mit ELISA durchgeführt. Eine Mischung aus Anti-Gm-Antikörper und zu untersuchendem Serum wurde in mit IgG oder seinen Fragmenten von passendem Gm-Typus überzogenen Mikrotitertüpfel inkubiert. Nach Waschen der Tüpfel wurde der gebundene Antikörper mit einem enzymisch markiertem zweiten Antikörper nachgewiesen. Bei dieser Teknik waren die Antigene Glm(3), G3m(16) und G3m(21) nachweisbar. Das ELISA-System mittels Anti-Gm-Antikörper ohne Anti-Rh0-Seren soll den Hämagglutinations-Hemmtest ersetzen, da einige humane Anti-Gm-Seren und Anti-Rh0-Seren nicht leicht erhältlich sind.

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Additional information

Supported in part by grants-in-aid for Scientific Research, nos. 58480206 and 59570267, from the Ministry of Education, Science and Culture

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Kishda, T., Tamaki, Y. Gm typing by enzyme-linked immunosorbent assay (ELISA). Z Rechtsmed 93, 23–27 (1984). https://doi.org/10.1007/BF00202980

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Key words

  • Forensic immunology Gm, ELISA
  • Enzyme-linked immunosorbent assay (ELISA), Gm

Schlüsselwörter

  • Forensische Immunologie, Gm-Typisierung (ELISA)
  • Gm-Typisierung, ELISA