Terminal meristems of Pisum sativum (garden pea) transit from vegetative to inflorescence development, and begin producing floral axillary meristems. Determination for inflorescence development was assessed by culturing excised buds and meristems. The first node of floral initiation (NFI) for bud expiants developing in culture and for adventitious shoots forming on cultured meristems was compared with the NFI of intact control buds. When terminal buds having eight leaf primordia were excised from plants of different ages (i.e., number of unfolded leaves) and cultured on 6-benzylaminopurine and kinetin-supplemented medium, the NFI was a function of the age of the source plant. By age 3, all terminal buds were determined for inflorescence development. Determination occurred at least eight nodes before the first axillary flower was initiated. Thus, the axillary meristems contributing to the inflorescence had not formed at the time the bud was explanted. Similar results were obtained for cultured axillary buds. In addition, meristems excised without leaf primordia from axillary buds three nodes above the cotyledons of age-3 plants gave rise to adventitious buds with an NFI of 8.3 ±0.3 nodes. In contrast seed-derived plants had an NFI of 16.5 ±0.2. Thus cells within the meristem were determined for inflorescence development. These findings indicate that determination for inflorescence development in P. sativum is a stable developmental state, separable from determination for flower development, and occurring prior to initiation of the inflorescence at the level of meristems.
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first node of floral initiation
Bhar, D.S. (1970) In vitro studies of floral shoot apices of Pharbitis nil. Can. J. Bot. 48, 1355–1358
Coen, E.S., Romero, J.M., Doyle, S., Elliott, R., Murphy, G., Carpenter, R. (1990) floricaula: a homeotic gene required for flower development in Antirrhinum majus. Cell 63, 1311–1322
Dennin, K.A., McDaniel, C.N. (1985) Floral determination in axillary buds of Nicotiana silvestris. Dev. Biol. 112, 377–382
Devlin, R.M., Witham, F.H. (1983) Plant Physiology, 4th edn., P.W.S. Publishers, Boston
Gebhardt, J.S., McDaniel, C.N. (1991) Flowering response of Nicotiana tabacum L. day-neutral and short-day cultivars: Interactions among roots, genotype, leaf ontogenic position and growth conditions. Planta 185, 513–517
Gould, K.S., Chambers, S.R.P. (1990) Characterization of pea shoots miniaturized by growth in culture. Plant Sci. 70, 121–127
Hackett, W.P., Sachs, R.M. (1968) Experimental separation of inflorescence development from initiation in Bougainvillea. J. Am. Soc. Hort. Sci. 92, 615–621
Habermann, H.M., Sekulow, D.B. (1972) Development and aging in Helianthus annuus L. Effects of the biological milieu of the apical meristem on patterns of development. Growth 36, 339–349
Irish, E.E., Nelson, T.M. (1988). Development of maize plants from cultured shoot apices. Planta 175, 9–12
Larkin, J.C., Felsheim, R., Das, A. (1990) Floral determination in the terminal bud of the short-day plant Pharbitis nil. Dev. Biol. 137, 434–443
Linsmaier, E.M., Skoog, F. (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18, 100–127
Matsushima, H., Itoyama, T., Mashiko, Y., Mizukoshi, T. (1974) Critical time of floral differentiation in Pharbitis nil shoot apex. In: Plant growth substances 1973, pp. 967–973, Hirakawa Publ., Tokyo
McDaniel, C.N. (1980) Influence of leaves and roots on meristem development in Nicotiana tabacum L. cv. Wisconsin 38. Planta 148, 462–467
McDaniel, C.N. (1984) Competence, determination and induction in plant development. In: Pattern formation: a primer in developmental biology, pp. 393–412, Malacinski, G.M., ed. Macmillan, New York
McDaniel, C.N. (1989) Floral initiation as a developmental process. In: Plant reproduction: from floral induction to pollination, pp. 51–57, Lord, E., Bernier, G., eds. American Society of Plant Physiologists, Rockville, Md.
McDaniel, C.N., King, R.W., Evans, L.T. (1991) Floral determination and in-vitro floral differentiation in isolated shoot apices of Lolium temulentum L. Planta 185, 9–16
Miginiac, E. (1978) Some aspects of regulation of flowering: role of correlative factors in photoperiodic plants. Bot. Mag. Tokyo, Spec. Issue 1, 159–173
Murfet, I.C. (1989) Flowering genes in Pisum. In: Plant reproduction: from floral induction to pollination, pp. 10–18, Lord, E., Bernier, G., eds. American Society of Plant Physiologists, Rockville, Md.
Paterson, K.E. (1984) Shoot tip culture of Helianthus annuus — flowering and development of adventitious and multiple shoots. Am. J. Bot. 71, 925–931
Paton, D.M. (1967) Leaf status and photoperiodic control of flower initiation in a late variety of pea. Nature 215, 319–320
Reid, J.B., Murfet, I.C. (1984) Flowering in Pisum: a fifth locus veg. Ann. Bot. 53, 369–382
Schwabe, W.W., Al-Doori, A.H. (1973) Analysis of a juvenile-like condition affecting flowering in the black currant (Ribes nigrum). J. Exp. Bot. 24, 969–981
Singer, S.R., McDaniel, C.N. (1986) Floral determination in the terminal and axillary buds of Nicotiana tabacum L. Dev. Biol. 118, 587–592
Singer, S.R., Hsiung, L.P., Huber, S.C. (1990) Determinate (det) mutant of Pisum sativum (Leguminosae: papilionoideae) exhibits an indeterminate growth pattern. Am. J. Bot. 77, 1330–1335
Smith, S.E.M. (1990) Leaf process and meristem determination in Nicotiana. M.S. thesis, Rensselaer Polytechnic Institute, Troy, N.Y., USA
Sokal, R.R., Rohlf, F.J. (1981) Biometry. Freemann, W.H., San Francisco
Sprent, J.I., Barber, H.N. (1957) Leaching of a flower inhibitor from late varieties of peas. Nature 180, 200–201
Wang, T.L., Woolhouse, H.W. (1982) Hormonal aspects of senescence in plant development. In: Growth regulators in plant senescence, pp. 5–25, Jackson, M.B., Grout, B., Mackenzie, I.A., eds. Monogr. 8, British Plant Growth Regulator Group, Wantage, UK
We thank Drs. Dorothy Jegla (Kenyon College, Gambier, Oh., USA), Michael Marcotrigiano (University of Massachussets, Amerherst, USA), and Carl McDaniel (Rensselaer Polytechnic Institute, Troy, N.Y., USA) for critical reviews of the manuscript and Ms. Heidi Junger for excellent plant care. This work was supported by U.S. Department of Agriculture grants 87-CRCR-1-2554 and 90-37261-5516 to S.R.S; C.J.F was supported by a Howard Hughes Medical Institute Undergraduate Biological Sciences Education Initiative Grant (71108-502901) and National Science Foundation grant DIR 90-00810.
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Ferguson, C.J., Huber, S.C., Hong, P.H. et al. Determination for inflorescence development is a stable state, separable from determination for flower development in Pisum sativum L. buds. Planta 185, 518–522 (1991). https://doi.org/10.1007/BF00202961
- Flower and inflorescence development (determination, inhibition)
- Pisum (flower and inflorescence determination)