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Determination for inflorescence development is a stable state, separable from determination for flower development in Pisum sativum L. buds

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Abstract

Terminal meristems of Pisum sativum (garden pea) transit from vegetative to inflorescence development, and begin producing floral axillary meristems. Determination for inflorescence development was assessed by culturing excised buds and meristems. The first node of floral initiation (NFI) for bud expiants developing in culture and for adventitious shoots forming on cultured meristems was compared with the NFI of intact control buds. When terminal buds having eight leaf primordia were excised from plants of different ages (i.e., number of unfolded leaves) and cultured on 6-benzylaminopurine and kinetin-supplemented medium, the NFI was a function of the age of the source plant. By age 3, all terminal buds were determined for inflorescence development. Determination occurred at least eight nodes before the first axillary flower was initiated. Thus, the axillary meristems contributing to the inflorescence had not formed at the time the bud was explanted. Similar results were obtained for cultured axillary buds. In addition, meristems excised without leaf primordia from axillary buds three nodes above the cotyledons of age-3 plants gave rise to adventitious buds with an NFI of 8.3 ±0.3 nodes. In contrast seed-derived plants had an NFI of 16.5 ±0.2. Thus cells within the meristem were determined for inflorescence development. These findings indicate that determination for inflorescence development in P. sativum is a stable developmental state, separable from determination for flower development, and occurring prior to initiation of the inflorescence at the level of meristems.

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Abbreviations

NFI:

first node of floral initiation

References

  1. Bhar, D.S. (1970) In vitro studies of floral shoot apices of Pharbitis nil. Can. J. Bot. 48, 1355–1358

  2. Coen, E.S., Romero, J.M., Doyle, S., Elliott, R., Murphy, G., Carpenter, R. (1990) floricaula: a homeotic gene required for flower development in Antirrhinum majus. Cell 63, 1311–1322

  3. Dennin, K.A., McDaniel, C.N. (1985) Floral determination in axillary buds of Nicotiana silvestris. Dev. Biol. 112, 377–382

  4. Devlin, R.M., Witham, F.H. (1983) Plant Physiology, 4th edn., P.W.S. Publishers, Boston

  5. Gebhardt, J.S., McDaniel, C.N. (1991) Flowering response of Nicotiana tabacum L. day-neutral and short-day cultivars: Interactions among roots, genotype, leaf ontogenic position and growth conditions. Planta 185, 513–517

  6. Gould, K.S., Chambers, S.R.P. (1990) Characterization of pea shoots miniaturized by growth in culture. Plant Sci. 70, 121–127

  7. Hackett, W.P., Sachs, R.M. (1968) Experimental separation of inflorescence development from initiation in Bougainvillea. J. Am. Soc. Hort. Sci. 92, 615–621

  8. Habermann, H.M., Sekulow, D.B. (1972) Development and aging in Helianthus annuus L. Effects of the biological milieu of the apical meristem on patterns of development. Growth 36, 339–349

  9. Irish, E.E., Nelson, T.M. (1988). Development of maize plants from cultured shoot apices. Planta 175, 9–12

  10. Larkin, J.C., Felsheim, R., Das, A. (1990) Floral determination in the terminal bud of the short-day plant Pharbitis nil. Dev. Biol. 137, 434–443

  11. Linsmaier, E.M., Skoog, F. (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18, 100–127

  12. Matsushima, H., Itoyama, T., Mashiko, Y., Mizukoshi, T. (1974) Critical time of floral differentiation in Pharbitis nil shoot apex. In: Plant growth substances 1973, pp. 967–973, Hirakawa Publ., Tokyo

  13. McDaniel, C.N. (1980) Influence of leaves and roots on meristem development in Nicotiana tabacum L. cv. Wisconsin 38. Planta 148, 462–467

  14. McDaniel, C.N. (1984) Competence, determination and induction in plant development. In: Pattern formation: a primer in developmental biology, pp. 393–412, Malacinski, G.M., ed. Macmillan, New York

  15. McDaniel, C.N. (1989) Floral initiation as a developmental process. In: Plant reproduction: from floral induction to pollination, pp. 51–57, Lord, E., Bernier, G., eds. American Society of Plant Physiologists, Rockville, Md.

  16. McDaniel, C.N., King, R.W., Evans, L.T. (1991) Floral determination and in-vitro floral differentiation in isolated shoot apices of Lolium temulentum L. Planta 185, 9–16

  17. Miginiac, E. (1978) Some aspects of regulation of flowering: role of correlative factors in photoperiodic plants. Bot. Mag. Tokyo, Spec. Issue 1, 159–173

  18. Murfet, I.C. (1989) Flowering genes in Pisum. In: Plant reproduction: from floral induction to pollination, pp. 10–18, Lord, E., Bernier, G., eds. American Society of Plant Physiologists, Rockville, Md.

  19. Paterson, K.E. (1984) Shoot tip culture of Helianthus annuus — flowering and development of adventitious and multiple shoots. Am. J. Bot. 71, 925–931

  20. Paton, D.M. (1967) Leaf status and photoperiodic control of flower initiation in a late variety of pea. Nature 215, 319–320

  21. Reid, J.B., Murfet, I.C. (1984) Flowering in Pisum: a fifth locus veg. Ann. Bot. 53, 369–382

  22. Schwabe, W.W., Al-Doori, A.H. (1973) Analysis of a juvenile-like condition affecting flowering in the black currant (Ribes nigrum). J. Exp. Bot. 24, 969–981

  23. Singer, S.R., McDaniel, C.N. (1986) Floral determination in the terminal and axillary buds of Nicotiana tabacum L. Dev. Biol. 118, 587–592

  24. Singer, S.R., Hsiung, L.P., Huber, S.C. (1990) Determinate (det) mutant of Pisum sativum (Leguminosae: papilionoideae) exhibits an indeterminate growth pattern. Am. J. Bot. 77, 1330–1335

  25. Smith, S.E.M. (1990) Leaf process and meristem determination in Nicotiana. M.S. thesis, Rensselaer Polytechnic Institute, Troy, N.Y., USA

  26. Sokal, R.R., Rohlf, F.J. (1981) Biometry. Freemann, W.H., San Francisco

  27. Sprent, J.I., Barber, H.N. (1957) Leaching of a flower inhibitor from late varieties of peas. Nature 180, 200–201

  28. Wang, T.L., Woolhouse, H.W. (1982) Hormonal aspects of senescence in plant development. In: Growth regulators in plant senescence, pp. 5–25, Jackson, M.B., Grout, B., Mackenzie, I.A., eds. Monogr. 8, British Plant Growth Regulator Group, Wantage, UK

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Author information

Correspondence to Susan R. Singer.

Additional information

We thank Drs. Dorothy Jegla (Kenyon College, Gambier, Oh., USA), Michael Marcotrigiano (University of Massachussets, Amerherst, USA), and Carl McDaniel (Rensselaer Polytechnic Institute, Troy, N.Y., USA) for critical reviews of the manuscript and Ms. Heidi Junger for excellent plant care. This work was supported by U.S. Department of Agriculture grants 87-CRCR-1-2554 and 90-37261-5516 to S.R.S; C.J.F was supported by a Howard Hughes Medical Institute Undergraduate Biological Sciences Education Initiative Grant (71108-502901) and National Science Foundation grant DIR 90-00810.

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Ferguson, C.J., Huber, S.C., Hong, P.H. et al. Determination for inflorescence development is a stable state, separable from determination for flower development in Pisum sativum L. buds. Planta 185, 518–522 (1991). https://doi.org/10.1007/BF00202961

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Key words

  • Flower and inflorescence development (determination, inhibition)
  • Pisum (flower and inflorescence determination)