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Comparative study of the sensitivity and specificity of the zinc and acid phosphatase spot tests for the detection of seminal stains

Summary

The sensitivity and specificity of a zinc spot test for the detection of semen were compared with those of an acid phosphatase detection method. As screening techniques both tests were found to be very sensitive, but the zinc test was more specific and was more reliable in older and especially in deteriorated specimens. It is concluded that the zinc spot test deserves at least the same place as the acid phosphatase test in the primary investigation of suspected semen stains and might well be the test of choice in older and poorly preserved stains.

Zusammenfassung

Sensitivität und Spezifität eines Fleckentests zum Spermanachweis auf der Basis der Zinkkonzentration im Samen wurden mit den genannten Gütekriterien der sauren Phosphatasetechnik verglichen. Beide Tests waren als Suchtests geeignet, aber der Zinktest erwies sich als spezifischer und zuverlässiger für ältere und denaturierte Proben. Demnach kommt dem Zinktest als Sperma-Suchtest zumindest derselbe Stellenwert zu, wie der Phosphatase-Methode. Bei alten und schlecht erhaltenen Spuren sollte dem Zinktest der Vorzug gegeben werden.

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Correspondence to P. Hooft.

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Hooft, P., van de Voorde, H. Comparative study of the sensitivity and specificity of the zinc and acid phosphatase spot tests for the detection of seminal stains. Z Rechtsmed 103, 581–586 (1990). https://doi.org/10.1007/BF00202718

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Key words

  • Acid phosphatase spot test
  • Zinc spot test
  • Seminal stains

Schlüsselwörter

  • Spermanachweis
  • Phosphatase-Methode
  • Zinktest, Spermasuchtest