We have isolated 4 cDNA clones (GRT1-4) encoding glutathione reductase (GR) from a tobacco (Nicotiana tabacum L.) leaf cDNA library. The cDNAs were almost identical: GRT1, GRT3 and GRT4 represented the same gene, differing only in that GRT4 contained an intron within the C-terminal part of the coding sequence. Failure to splice out this intron resulted in a substitution of the final 13 amino acids of the deduced amino acid sequence. A second gene was represented by GRT2. Southern blots indicated that there were two related GR genes in tobacco. The presence of multiple isoforms of GR in tobacco may be explained in part by the expression of a small gene family. In addition, alternative isoforms may result from translation of different mRNAs derived from the same gene by intron skipping during the splicing of nascent GR mRNAs.
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rapid amplification of cDNA ends
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We thank Dr. Rod Casey for critical reading of the manuscript. This work was supported by the Biotechnology and Biological Sciences Research Council through a grant-in-aid to the John Innes Centre and by the Commission of the European Union Frame-work III AIR programme (contract number AIR1-CT92-0205)
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Creissen, G.P., Mullineaux, P.M. Cloning and characterisation of glutathione reductase cDNAs and identification of two genes encoding the tobacco enzyme. Planta 197, 422–425 (1995). https://doi.org/10.1007/BF00202667
- Glutathione reductase (cDNAs)
- Intron skipping