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Manganese reduction in the rhizosphere of mycorrhizal and nonmycorrhizal maize

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The influence of rhizosphere microorganisms and vesicular-arbuscular (VA) mycorrhiza on manganese (Mn) uptake in maize (Zea mays L. cv. Tau) plants was studied in pot experiments under controlled environmental conditions. The plants were grown for 7 weeks in sterilized calcareous soil in pots having separate compartments for growth of roots and of VA mycorrhizal fungal hyphae. The soil was left either uninoculated (control) or prior to planting was inoculated with rhizosphere microorganisms only (MO-VA) or with rhizosphere microorganisms together with a VA mycorrhizal fungus [Glomus mosseae (Nicol and Gerd.) Gerdemann and Trappe] (MO+VA). Mycorrhiza treatment did not affect shoot dry weight, but root dry weight was slightly inhibited in the MO+VA and MO-VA treatments compared with the uninoculated control. Concentrations of Mn in shoots decreased in the order MO-VA > MO+VA > control. In the rhizosphere soil, the total microbial population was higher in mycorrhizal (MO+VA) than nonmycorrhizal (MO-VA) treatments, but the proportion of Mn-reducing microbial populations was fivefold higher in the nonmycorrhizal treatment, suggesting substantial qualitative changes in rhizosphere microbial populations upon root infection with the mycorrhizal fungi. The most important microbial group taking part in the reduction of Mn was fluorescent Pseudomonas. Mycorrhizal treatment decreased not only the number of Mn reducers but also the release of Mn-solubilizing root exudates, which were collected by percolation from maize plants cultivated in plastic tubes filled with gravel quartz sand. Compared with mycorrhizal plants, the root exudates of nonmycorrhizal plants had two fold higher capacity for reduction of Mn. Therefore, changes in both rhizosphere microbial population and root exudation are probably responsible for the lower acquisition of Mn in mycorrhizal plants.

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  1. Arines J, Vilarino A, Sainz M (1989) Effect of different inocula of vesicular-arbuscular mycorrhizal fungi on manganese content and concentration in red clover (Trifolium pratense L.) plants. New Phytol 112:215–219

  2. Arines J, Porto ME, Vilarino A (1992) Effect of manganese on vesicular-arbuscular mycorrhizal development in red clover plants and on soil Mn-oxidizing bacteria. Mycorrhiza 1:127–131

  3. Bromfield SM, David DJ (1976) Sorption and oxidation of Mn and reduction of MnO2 by suspension of manganese oxidizing bacteria. Soil Biochem 8:37–43

  4. Dixon RK, Garrett HE (1989) Boron fertilization, vesicular-arbuscular mycorrhizal colonization and growth of Citrus jambhiri Lush. J Plant Nutr 12:687–700

  5. Dixon RK, Garrett H, Cox GS (1988) Carbohydrate relationships of Citrus jambhiri inoculated with Glomus fasciculatum. J Am Soc Hortic Sci 113:239–242

  6. Ehrlich HL (1980) Bacterial leaching of manganese ores. In: Trudinger PA, Walter MR, Ralph BJ (eds) Biochemistry of ancient and modern environments. Springer, New York Berlin Heidelberg, pp 609–614

  7. Ghiorse WC, Ehrlich HL (1976) Electron transport component of the MnO2 reductase system and the location of the terminal reductase in a marine bacillus. Appl Environ Microbiol 31:977–985

  8. Gioyannetti M, Mosse B (1980) An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infections in roots. New Phytol 84:489–500

  9. Gnekow MA, Marschner H (1989) Role of VA-mycorrhiza in growth and mineral nutrition of apple (Malus pumila var. domestica) rootstock cuttings. Plant Soil 119:185–293

  10. Gode GH, Reisenauer HM (1980) Plant effects on soil manganese availability. Soil Sci Soc Am 44:993–995

  11. Gomah AM, Awad Soliman GS, Abdel-Ghaffar AS (1980) Manganese mobility in Egyptian soils as affected by inoculation with manganese reducing organisms. Z Pflanzenernähr Bodenkd 143:274–281

  12. Hagedorn C, Holt JG (1975) Ecology of soil arthrobacter in Clarion-Webster topoguences of Iowa. Appl Microbiol 29:211–218

  13. Jauregui MA, Reisenauer HM (1982) Dissolution of oxides of manganese and iron by root exudates components. Soil Sci Am J 467:314–317

  14. King EO, Ward MK, Raney DE (1954) Two simple methods for the determination of pyocyanin and fluorescin. J Lab Clin Med 44:301–304

  15. Kothari SK, Marschner H, Römheld V (1990) Direct and indirect effects of VA mycorrhizal fungi and rhizosphere microorganisms on acquisition of mineral nutrients by maize (Zea mays L.) in a calcareous soil. New Phytol 116:637–645

  16. Kothari SK, Marschner H, Römheld V (1991) Effect of a vesicular-arbuscular mycorrhizal fungus and rhizosphere micro-organisms on manganese reduction in the rhizosphere and manganese concentrations in maize (Zea mays L.). New Phytol 117:649–655

  17. Krishna KR, Balakrishna AN, Bagyaraj DJ (1982) Interaction between a vesicular-arbuscular mycorrhizal fungus and Streptomyces cinnamomeous and their effect on finger millet. New Phytol 92:401–405

  18. Li XL, George E, Marschner H (1991) Phosphorus depletion and pH decrease at the root-soil and hyphae-soil interfaces of VA mycorrhizal white clover fertilized with ammonium. New Phytol 119:397–404

  19. Linderman RG (1988) Mycorrhizal interactions with the rhizosphere microflora: the mycorrhizosphere effect. Phytopathology 78:366–371

  20. Lingappa Y, Lockwood JL (1962) Chitin media for selective isolation and culture of actinomycetes. Phytopathology 52:317–323

  21. Marschner H (1988) Mechanisms of manganese acquisition by roots from soils. In: Graham RD, Hannam RJ, Uren NC (eds) Manganese in soils and plants. Kluwer, London, pp 191–204

  22. Martin JK (1975) Comparison of agar media for counts of viable soil bacteria. Soil Biol Biochem 7:401–402

  23. Meyer JR, Linderman RG (1986) Selective influence on populations of rhizosphere or rhizoplane bacteria and actinomycetes by mycorrhizas formed by Glomus fasciculatum. Soil Biol Biochem 18:191–196

  24. Miller HJ, Henken G, Veen JA van (1989) Variation and composition of bacterial populations in the rhizospheres of maize, wheat and grass cultivars. Can J Microbiol 35:656–660

  25. Miller HJ, Liljeroth E, Henken G, Veen JA van (1990) Fluctuations in the fluorescent pseudomonad and actinomycetes populations of rhizosphere and rhizoplane during the growth of spring wheat. Can J Microbiol 36:254–258

  26. Pacovsky RS (1986) Micronutrient uptake and distribution in mycorrhizal or phosphorus fertilized soybeans. Plant Soil 95:379–388

  27. Paulitz TC, Linderman RG (1989) Interactions between fluorescent pseudomonads and VA mycorrhizal fungi. New Phytol 113:37–45

  28. Ridge EH, Rovira AD (1971) Phosphatase activity of intact young wheat roots under non-sterile conditions. New Phytol 70:1017–1020

  29. Rovira AD, Newman EL, Bowen HJ, Campbell R (1974) Quantitative assessment of the rhizoplane microflora by direct microscopy. Soil Biol Biochem 6:211–216

  30. Secilia JM, Bagyaraj DJ (1987) Bacteria and actinomycetes associated with pot cultures of vesicular-arbuscular mycorrhizas. Can J Microbiol 33:1069–1073

  31. Sparrow LA, Uren NC (1987) Oxidation and reduction of Mn in acidic soils: effect of temperature and soil pH. Soil Biol Biochem 19:143–148

  32. Tennant D (1975) A test of modified line intersect method of estimating root length. J Ecol 63:995–1001

  33. Uren NC (1981) Chemical reduction of an insoluble higher oxide of manganese by plant roots. J Plant Nutr 4:65–71

  34. Uren NC, Reisenauer HM (1988) The role of root exudates in nutrient acquisition. In: Tinker E, Lauchli A (eds) Advances in plant nutrition, vol 3. Praeger, New York, pp 798–814

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Posta, K., Marschner, H. & Römheld, V. Manganese reduction in the rhizosphere of mycorrhizal and nonmycorrhizal maize. Mycorrhiza 5, 119–124 (1994). https://doi.org/10.1007/BF00202343

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Key words

  • Glomus mosseae
  • Manganese uptake
  • Root exudation
  • Manganese reduction
  • Mycorrhizal effect
  • Zea mays