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Incidence of chromosome 18 disomy in human sperm nuclei as detected by nonisotopic in situ hybridization

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Abstract

Nonradioactive in situ hybridization with an α-satellite DNA probe specific for chromosome 18 was performed on human interphase sperm nuclei to detect the frequency of sperm cells disomic for chromosome 18. A total of 16127 sperm heads from eight healthy donors, aged 23–57 years, was investigated, and a minimum of 2000 sperm nuclei per proband was analyzed. The disomy rate ranged from 0.25% to 0.5%, with an average of 0.36%. This frequency does not differ significantly from that determined for other chromosomes.

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Correspondence to Michael Schmid.

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Guttenbach, M., Schakowski, R. & Schmid, M. Incidence of chromosome 18 disomy in human sperm nuclei as detected by nonisotopic in situ hybridization. Hum Genet 93, 421–423 (1994). https://doi.org/10.1007/BF00201667

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Keywords

  • Internal Medicine
  • Metabolic Disease
  • Healthy Donor
  • Sperm Cell
  • Human Sperm