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Ein neues kriminalistisches verfahren: Untersuchung der l-cystin-aminopeptidase-Aktivität zur beweisführung von spermaflecken

A new criminological procedure: Investigation of l-cystine-aminopeptidase activity for the detection of seminal stains

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Summary

l-Cystine-aminopeptidase (CAP) enzyme cleaves l-cystine-di-β-naphthylamide substrate to β-naphthylamine. The final product of this process is a violet azostain. CAP enzyme determination was developed for criminological procedures. CAP shows almost 100 times greater activity in human semen than in other body fluids. Samples taken at different time intervals (1 week, 1–3–6 months, 1 year, and 5–10 years) were investigated; they were dried on cloth and filter paper and then stored at room temperature for various time periods. The enzyme was also shown to be specific in the comparative studies performed with other human secretions (nasal discharge, saliva, vaginal secretion, feces, urine, breast milk) and fruit juices (raspberry, strawberry, apple, pear, plum, tomato, etc.).

Zusammenfassung

Das l-Cystin-Aminopeptidase (CAP)-Enzym spaltet das l-Cystin-di-β-naphthylamid-Substrat zu β-Naphthylamin; als Endprodukt dieser Diazoreaktion entsteht ein violetter Azofarbstoff. Für die kriminalistische Praxis wurde eine qualitative Methode zur CAP-Enzymbestimmung erarbeitet. Die Möglichkeit hierzu war vor allem dadurch gegeben, daß die CAP im Samen eine fast hundertfach größere Aktivität zeigt als in anderen menschlichen Körpersekreten. Untersucht wurden auf Textil- und Filterpapier eingetrocknete und verschieden lange Zeit bei Raumtemperatur gelagerte Proben unterschiedlichen Alters (1 Woche, 1–3–6 Monate, 1 sowie 5–10 Jahre). Das Enzym erwies sich auch im Laufe der mit anderweitigen humanen Körpersäften (Nasensekret, Speichel, Scheidensekret, Stuhl, Urin, Muttermilch usw.) und pflanzlichen Säften (Himbeeren, Erdbeeren, Äpfel, Birnen, Pflaumen, Tomaten usw.) angestellten vergleichenden Untersuchungen als spezifisch.

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Correspondence to F. Kósa.

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Kósa, F., Rengei, B. & Szendrényi, J. Ein neues kriminalistisches verfahren: Untersuchung der l-cystin-aminopeptidase-Aktivität zur beweisführung von spermaflecken. Z Rechtsmed 98, 255–262 (1987). https://doi.org/10.1007/BF00201232

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Key words

  • l-Cystine-aminopeptidase (CAP) activity
  • Human seminal fluids
  • Seminal stains, detection for criminological purposes

Schlüsselwörter

  • l-Cystin-Aminopeptidase (CAP)-Aktivität, in Samenflüssigkeit und Samenflecken
  • Samenflüssigkeit (-Flecken), Cystin
  • Aminopeptidase-Aktivität