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Cathepsin D: A new marker of the vitality of the wound

Comparative study with histamine and serotonin

Summary

We have studied the variation of specific activity of the most important lysosomal enzyme: Cathepsin D. This study has been developed in pig skin incision wounds for its application to the differential diagnosis between vital and postmortem wounds. At the same time, we have determined histamine and serotonine levels to make a comparative study with Cathepsin results.

Our results show the utility of this new marker to determine the vitality of wounds, even when the time elapsed until death has been very short.

Nevertheless, there is no correlation between Cathepsin D results and those of histamine or serotonin, a fact that induces us to think that this new marker is not useful to determine wound age.

Zusammenfassung

Wir haben die Variation der spezifischen Aktivität des wichtigsten lysosomalen Enzyms untersucht: Cathepsin D. Diese Untersuchung ist in Schnittwunden in Schweinehaut entwickelt worden im Hinblick auf die Anwendung der Methode zur Differentialdiagnose zwischen vitalen und postmortalen Wunden. Gleichzeitig wurden die Histamin- und Serotonin-Konzentrationen bestimmt, um einen Vergleich mit den Cathepsin-Ergebnissen zu ermöglichen. Unsere Ergebnisse zeigen die Brauchbarkeit dieses neuen Markers, um die Vitalität von Wunden zu bestimmen, auch bei sehr kurzen Überlebenszeiten. Trotzdem besteht keine Korrelation zwischen den Cathepsin-D-Ergebnissen und den Bestimmungen der Histaminund Serotonin-Gehalte. Das ist ein Befund, der uns daran denken läßt, daß dieser neue Marker nicht zur Wundaltersbestimmung geeignet ist.

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Correspondence to J. A. Lorente.

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Lorente, J.A., Hernandez-Cueto, C. & Villanueva, E. Cathepsin D: A new marker of the vitality of the wound. Z Rechtsmed 98, 95–101 (1987). https://doi.org/10.1007/BF00200465

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Key words

  • Vitality of wounds, Cathepsin D
  • Cathepsin D, marker of the vitality of wounds

Schlüssenwörter

  • Vitale Reaktion, Cathepsin D
  • Cathepsin D, ein Marker zur Vitalitätsbestimmung