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Hepatitis a virus infection of HBsAg-producing hepatocellular carcinomas in athymic mice

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Summary

Two human hepatocellular carcinoma cell lines were grown in athymic mice. Morphologically, the tumors resembled hepatocellular carcinomas. HBsAg, anti-HBs, and α-fetoprotein were detected in sera of mice bearing tumors of PLC/PRF/5 or Hep 3B cells; their amounts correlated with tumor weight. Tumors of both cell lines were infected with cell culture-adapted hepatitis A virus (HAV); HAV antigen and infectious virus were recovered from infected tumors of PLC/PRF/5 and Hep 3B cells. Our results indicate that HAV-infected tumors may be useful in studying the production in vivo of hepatitis A virus antigen.

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References

  1. 1.

    Aden DP, Fogel A, Plotkin A, Damjanov I, Knowles BB (1979) Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line. Nature 282:615

  2. 2.

    Alexander JJ, Macnab G, Saunders R (1978) Studies on in vitro production of hepatitis B surface antigen by a human hepatoma cell line. Perspectives in Virology 10:103

  3. 3.

    Bassendine MF, Arborgh BAM, Shipton N, Monjardino J, Aranguibel F, Thomas HC, Sherlock S (1980) Hepatitis B surface antigen and alpha-fetoprotein secreting human primary liver cell cancer in athymic mice. Gastroenterology 79:528

  4. 4.

    Deinhardt F, Scheid R, Gauss-Müller V, Frösner GG, Siegl G (1980) Propagation of human hepatitis A virus in cell lines of primary human hepatocellular carcinomas. Prog Med Virol 27:109

  5. 5.

    Desmyter J, Ray MB, Bradburne AF, Alexander JJ (1978) Human HBsAg-positive hepatoma in nude athymic mice. In: Viral hepatitis. Franklin Institute Press, Philadelphia, p 459

  6. 6.

    Frösner GG, Deinhardt F, Scheid R, Gauss-Müller V, Holmes N, Messelberger V, Siegl G, Alexander JJ (1979) Propagation of human hepatitis A virus in a hepatoma cell line. Infection 7:303

  7. 7.

    Gauss-Müller V, Frösner GG, Deinhardt F (1981) Propagation of hepatitis A virus in human embryo fibroblasts. J Med Virol 7:232

  8. 8.

    Hahn EG, Gauss-Müller V, Hornberger M, Kalbfleisch H, Deinhardt F (1982) Solide Tumoren menschlicher primärer hepatozellulärer Karzinomzellinien in hypothymischen Mäusen: ein Modell für biochemische und therapeutische Studien. Z Gastroenterol (in press)

  9. 9.

    Minato N, Bloom BR, Jones C, Holland I, Reid LM (1979) Mechanism of rejection of virus persistently infected tumor cells by athymic nude mice. J Exp Med 149:1117

  10. 10.

    Reid LM, Jones CL, Holland I (1979) Virus carrier state suppresses tumorigenicity of tumor cells in athymic (nude) mice. J Gen Virol 42:609

  11. 11.

    Shouval D, Reid LM, Chaleraborty PR, Ruiz-Oparo N, Morechi R, Gerber MA, Thung SN, Shafritz DA (1981) Tumorigenicity in nude mice of a human hepatoma cell line containing hepatitis B virus DNA. Cancer Res 41:1342

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Correspondence to Verena Gauss-Müller.

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Gauss-Müller, V., Deinhardt, F., Hahn, E. et al. Hepatitis a virus infection of HBsAg-producing hepatocellular carcinomas in athymic mice. Cancer Immunol Immunother 13, 53–55 (1982). https://doi.org/10.1007/BF00200201

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Keywords

  • Carcinoma
  • Hepatitis
  • Carcinoma Cell
  • Virus Infection
  • Hepatocellular Carcinoma