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Accumulation of scopoletin is associated with the high disease resistance of the hybrid Nicotiana glutinosa x Nicotiana debneyi

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Abstract

The high disease resistance of the amphidiploid hybrid of Nicotiana glutinosa x Nicotiana debneyi is associated with high constitutive levels of two phenolic compounds as analysed by high-performance liquid chromatography. The structures of these two compounds were elucidated by means of gas chromatography-tandem mass spectrometry, fluorescence- and light-spectrophotometry to be those of scopolin and scopoletin. They reached levels of 4 nmol·(g FW)−1 and 35 nmol·(g FW)−1, respectively, in leaf tissues of the hybrid, about 10–50 times the amount found in the parental species. Scopoletin showed a direct antimicrobial activity against Cercospora nicotianae, Phytophthora parasitica var. nicotianae, Pseudomonas syringae pvs. tabaci and syringae and tobacco mosaic virus when added to synthetic growth media, mixed with the inoculum or sprayed onto tobacco plants prior to inoculation. We postulate that the high amount of toxic phenolics in the leaves of the hybrid N. glutinosa x N. debneyi contributes to its high disease resistance.

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Abbreviations

GC/MS-MS:

gas chromatography — tandem mass spectrometry

HPLC:

high-performance liquid chromatography

PR-proteins:

pathogenesis-related proteins

TMV:

tobacco mosaic virus

TNV:

tobacco necrosis virus

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Author information

Correspondence to P. Ahl Goy.

Additional information

We thank Dr. W. Fischer (Ciba-Geigy, Plant Protection Division, Basle, Switzerland) for critical review of the manuscript.

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Goy, P.A., Signer, H., Reist, R. et al. Accumulation of scopoletin is associated with the high disease resistance of the hybrid Nicotiana glutinosa x Nicotiana debneyi . Planta 191, 200–206 (1993). https://doi.org/10.1007/BF00199750

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Key words

  • Disease resistance
  • Nicotiana
  • Scopoletin
  • Scopolin