Cancer Immunology, Immunotherapy

, Volume 11, Issue 4, pp 243–250 | Cite as

Serologic analysis of human tumor antigens

III. Reactivity of patients with melanoma and osteogenic sarcoma to cultured tumor cells and fibroblasts in the immune adherence assay
  • Stephen F. Sener
  • John M. Brown
  • Cornelia L. Hyatt
  • William D. Terry
  • Steven A. Rosenberg
Original Articles


The immune adherence (IA) assay was used to measure serum reactivity of patients with melanoma and osteosarcoma against paired cell lines (tumor cells and normal skin fibroblasts obtained from the same individuals) grown in tissue culture. Sera from 224 patients with various stages of melanoma were compared with sera from 100 normal age- and sex-matched donors. None of the 18 stage I sera (0%), 23 of 166 (14%) stage II sera, 3 of 40 (7%) stage III sera, and 3 of 100 (3%) normal sera were highly reactive to a standard allogeneic melanoma-fibroblast pair. None of the sera exhibited unique activity against melanoma. There was no correlation between stage of melanoma and high serum reactivity, nor was this reactivity predictive of recurrence. Sera from 39 tumor-bearing osteosarcoma patients prior to amputation were compared with sera from 50 normal age-and sex-matched donors. Eight of 39 (21%) patient sera and 1 of 50 (2%) normal sera were highly reactive to an osteosarcoma-fibroblast pair. No sera had reactivity uniquely directed against osteosarcoma. Eight osteosarcoma and two melanoma patients were tested simultaneously against their autologous cultured tumor and skin cells. Only one of these patients exhibited high reactivity towards autologous cells, and this reactivity was equal against both osteosarcoma and normal cells. None of seven highly reactive osteosarcoma or six highly reactive melanoma sera had residual tumor-specific reactivity against allogeneic osteosarcoma or melanoma after absorption with cultured fibroblasts, cultured fetal fibroblasts, or fetal calf serum.


Melanoma Osteosarcoma Tumor Antigen Melanoma Patient Skin Fibroblast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Stephen F. Sener
    • 1
  • John M. Brown
    • 1
  • Cornelia L. Hyatt
    • 1
  • William D. Terry
    • 2
  • Steven A. Rosenberg
    • 1
  1. 1.Surgery BranchNational Cancer Institute, National Institutes of HealthBethesdaUSA
  2. 2.Immunology BranchNational Cancer Institute, National Institutes of HealthBethesdaUSA

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