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Effect of plant growth regulators on ethylene production, 1-aminocyclopropane-1-carboxylic acid oxidase activity, and initiation of inflorescence development of pineapple

Abstract

With the development of pineapple [Ananas comosus (L.) Merr.] as a fresh fruit crop, it became common to force inflorescence development with ethephon [(2-chloroethyl)phosphonic acid] or ethylene throughout the year. Environmental induction (EI) of inflorescence development disrupts scheduling of fruit harvest and may cause significant losses if small plants are induced, resulting in fruits that are too small to be marketable. Our objective was to identify plant growth regulators (PGRs) that could inhibit EI. Because circumstantial evidence indicates that EI occurs in response to naturally produced ethylene or changes in plant sensitivity to it, most work was done with PGRs that inhibit ethylene biosynthesis or block ethylene action. The synthetic auxin 2-(3-chlorophenoxy)propionic acid (CPA) was included because in one study it reduced the percentage of EI. GA3, aminooxyacetic acid (AOA), aminoethoxyvinylglycine (AVG), daminozide [butanedioic acid mono-(2,2-dimethylhydrazide)], and silver thiosulfate (STS) had no effect on EL CPA, paclobutrazol [(2RS,3RS)-1-(4-chlorophenyl)methyl-4,4-dimethyl-2(1h-1,2,4-triazol-1-yl)penten-3-ol], and uniconazole [(E)-(p-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-ol] delayed or inhibited EI of pot-grown pineapple plants. Uniconazole and paclobutrazol inhibited growth and ethylene production by leaf basal-white tissue, and either or both effects could account for the inhibition of EI. Production of 1-aminocyclopropane-1-carboxylic acid (ACC) was unaffected by these compounds, but the activity of ACC oxidase, which converts ACC to ethylene, was inhibited and probably accounts for the reduced ethylene production by leaf basal-white tissue. CPA stimulated ethylene production by stem apical tissue approximately fourfold relative to the control. ACC oxidase activity and the malonyl-ACC (MACC) content in stem apical tissue were also greater than in the control, indicating that CPA greatly stimulated the production of ACC and its sequestration into MACC. The mechanism by which CPA delayed or inhibited EI is not known. CPA, paclobutrazol, and uniconazole appear to have some potential for inhibiting EI of pineapple. Their effect on yield needs to be determined.

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Abbreviations

ACC oxidase:

1-aminocyclopropane-1-carboxylic acid oxidase

CPA:

2-(3-chlorophenoxy)propionic acid

AOA:

aminooxyacetic acid

AVG:

aminoethoxyvinylglycine

daminozide:

butanedioic acid mono-(2,2-dimethylhydrazide)

DM:

dry mass

ethephon:

[(2-chloroethyl)phosphonic acid]

FM:

fresh mass

GA:

gibberellin

EI:

environmental induction of inflorescence development

IA:

inflorescence appearance

LSD:

Fisher's protected least significant difference

MACC:

malonyl-ACC

NAA:

naphthaleneacetic acid

PGR:

plant growth regulator

paclobutrazol:

(2RS,3RS)-1-(4-chlorophenyl)methyl-4,4-dimethyl-2-(1h-1,2,4-triazol-1-yl)penten-3-ol]

uniconazole:

(E)-(p-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-ol

STS:

silver thiosulfate

M-leaf:

fourth leaf

Ml-L:

first leaf younger than M-leaf

References

  1. Abeles FB, Morgan PW, Saltveit ME Jr (1992) Ethylene in plant biology. 2nd ed. Academic Press, San Diego

  2. Bartholomew DP (1977) Inflorescence development of pineapple [Ananas comosus (L.) Merr.] induced to flower with ethephon. Bot Gaz 138:312–320

  3. Bartholomew DP (1985) Ananas comosus. In: Halevy AH (ed) Handbook of flowering, Vol 1. CRC Press, Boca Raton, FL pp. 450–454

  4. Bartholomew DP, Criley RA (1983) Tropical fruit and beverage crops. In: Nickell LG (ed) Plant growth regulating chemicals. Vol 2. CRC Press, Boca Raton, FL pp. 1–11

  5. Bartholomew DP, Malézieux E (1994) Pineapple. In: Schaffer B, Andersen PC (eds) Handbook of environmental physiology of fruit crops. Vol. 2. Subtropical and tropical crops. CRC Press, Boca Raton, FL, pp. 243–291

  6. Bartholomew DP, Paull RE (1986) Pineapple. In: Monselise SP (ed) Handbook of fruit set and development. CRC Press, Boca Raton, FL, pp. 371–388

  7. Burg SP, Burg EA (1966) Auxin-induced ethylene formation: Its relation to flowering in the pineapple. Science 152:1269

  8. Davis TD, Curry EA (1991) Chemical regulation of vegetative growth. Crit Rev Plant Sci 10:151–188

  9. Davis TD, Steffens GL, Sankhla N (1988) Triazole plant growth regulators. Hortic Rev 10:63–105

  10. Gowing DP (1956) A hypothesis of the role of naphthaleneacetic acid in the flower induction on the pineapple. Am J Bot 43:411–418

  11. Gowing DP (1961) Experiments on the photoperiodic response in pineapple. Am J Bot 48:16–21

  12. Gowing DP, Leeper RW (1960) Studies on the relation of chemical structure to plant growth regulator activity in the pineapple plant. I. Substituted phenyl and phenoxyalkylcarboxylic acids. Bot Gaz 121:143–151

  13. Grossmann K (1990) Plant growth retardants as tools in physiological research. Physiol Plant 78:640–648

  14. Grossmann K, Hauser C, Sauerbrey D, Fritsch H, Schmidt O, Jung J (1989) Plant growth retardants as inhibitors of ethylene production. J Plant Physiol 134:538–543

  15. Gussman CD, Salas S, Gianfagna TJ (1993) Daminozide inhibits ethylene production in apple fruit by blocking the conversion of methionine to aminocyclopropane-1-carboxylic acid (ACC). Plant Growth Regul 12:149–154

  16. Hoffman NE, Yang SF, McKeon T (1982) Identification of 1-(malonylamino)cyclopropane-1-carboxylic acid as a major conjugate of 1-aminocyclopropane-1-carboxylic acid, an ethylene precursor in higher plants. Biochem Biophys Res Commun 104:765–770

  17. Hofstra G, Krieg LC, Fletcher RA (1989) Uniconazole reduces ethylene and 1-aminocyclopropane-1-carboxylic acid and increases spermine levels in mung bean seedlings. J Plant Growth Regul 8:45–51

  18. Izumi K, Nakagawa S, Kobayashi M, Oshio H, Sakurai A, Takahashi N (1988) Levels of IAA, cytokinins, ABA, and ethylene in rice plants as affected by a gibberellin biosynthesis inhibitor, uniconazole-P. Plant Cell Physiol 29:97–104

  19. Krause TE, Murr DP, Fletcher RA (1991) Uniconazole inhibits stress-induced ethylene in wheat and soybean seedlings. J Plant Growth Regul 10:229–234

  20. Leeper RW (1965) Factors influencing forcing and delaying: A review. PRI News 13:109–121 (unpublished report of the Pineapple Research Institute of Hawaii)

  21. Lizada MC, Yang SF (1979) A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid. Anal Biochem 100:140–145

  22. Lurssen K (1987) The use of inhibitors of gibberellin and sterol biosynthesis to probe hormone action. In: Hoad GV, Lenton JR, Jackson MB, Atkin RD (eds) Hormone action in plant development: A critical appraisal. Butterworths, London, U.K. pp. 133–144

  23. Mekers O, De Proft M, Jacobs L (1983) Prevention of unwanted flowering of ornamental Bromeliaceae by growth regulating chemicals. Acta Hort 137:217–223

  24. Millar-Watt D (1981) Control of natural flowering in Smooth Cayenne pineapple, Ananas comosus (L.) Merr. Subtropica 2:17–19

  25. Min XJ, Bartholomew DP (1993) Effects of growth regulators on ethylene production and floral initiation of pineapple. Acta Hort 334:101–112

  26. Sanford WG, Bartholomew DP (1981) Effects of silver and cobalt ions on floral induction of pineapple by ethephon. HortScience 16:442

  27. Scott CH (1993) The effect of two plant growth regulators on the inhibition of precocious fruiting in pineapple. Acta Hort 334:77–82

  28. Sitrit Y, Riov J, Blumenfeld A (1988) Interference of phenolic compounds with the 1-aminocyclopropane-1-carboxylic acid assay. Plant Physiol 86:13–15

  29. Starrett DA, Laties GG (1991) The effect of ethylene and propylene pulses on respiration, ripening advancement, ethylene-forming enzyme, and 1-aminocyclopropane-1-carboxylic acid synthase activity in avocado fruit. Plant Physiol 95:921–927

  30. Suwunnamek U (1993) Effect of paclobutrazol, thiourea, and pendimethalin alone or in combination on the induction of suckering in pineapple. Acta Hort 334:93–99

  31. Van Overbeek J, Cruzado HJ (1948) Flower formation in the pineapple plant by geotropic stimulation. Am J Bot 35:410–412

  32. Wang SY, Steffens GL (1985) Effect of paclobutrazol on water stress-induced ethylene biosynthesis and polyamine accumulation in apple seedling leaves. Phytochemistry 24:2185–2190

  33. Yamaji H, Katsura N, Nishijima T, Koshioka M (1991) Effects of soil applied uniconazole and prohexadione calcium on the growth and endogenous gibberellin content of Lycopersicon esculentum Mill, seedlings. J Plant Physiol 138:763–764

  34. Yang SF, Hoffman NE (1984) Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol 35:155–189

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Correspondence to D. P. Bartholomew.

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Min, X., Bartholomew, D.P. Effect of plant growth regulators on ethylene production, 1-aminocyclopropane-1-carboxylic acid oxidase activity, and initiation of inflorescence development of pineapple. J Plant Growth Regul 15, 121 (1996). https://doi.org/10.1007/BF00198926

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Key Words

  • Pineapple flower induction
  • Paclobutrazol
  • Uniconazole
  • Ethylene production