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Regulation of flower development in cultured ears of maize (Zea mays L.)

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Summary

Young ears of maize were cultured in two different liquid media containing either kinetin (KN) or kinetin + gibberellic acid (KN + GA3) in order to manipulate stamen and gynoecium development. In KN medium, stamens developed and gynoecia aborted in the flowers of the cultured immature ears. In the KN + GA3 medium, however, ovaries with silks developed and stamens aborted. These differential morphological events were recorded with SEM photomicrographs at regular intervals after excision of ear inflorescences. In addition, the mitotic activity in the developing or aborting organs was determined over a 75-h period. It increased from 6% to 14% in developing organs (i.e. stamens in KN medium, and gynoecia in KN + GA3 medium) and gradually decreased to 1% in the degenerating organs (i.e. gynoecia in KN medium, and stamens in KN + GA3 medium) by 45 h of culture. The mitotic activity reached zero in degenerating flower organs by 75 h of culture. Whether these differential sensitivities to the exogenously applied members of these two plant growth regulator classes are unique to our in vitro system or reflect a more general control feature of in vivo inflorescences must await further clarification.

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References

  1. Bommineni VR (1988) Regulation of flower development in cultured inflorescences of maize (Zea mays L.). PhD thesis, The University of Western Ontario, London, Ontario, Canada. Diss Abstr Int B 49:1463

  2. Bommineni VR, Greyson RI (1987) In vitro culture of ear shoots of Zea mays and the effect of kinetin on sex expression. Am J Bot 74:883–890

  3. Bommineni VR, Greyson RI, Walden DB, Atkinson BG (1989) Polypeptides synthesized during the maturation of flower organs from tassel and ear inflorescences of Zea mays L. Maydica

  4. Bonnett OT (1940) Development of the staminate and pistillate inflorescences of sweet corn. J Agric Res 60:25–37

  5. Bonnett OT (1948) Ear and tassel development in maize. Ann Mo Bot Gard 35:269–288

  6. Bonnett OT (1953) Developmental morphology of the vegetative and floral shoots of maize. Univ Ill Agric Exp Stn Bull 568

  7. Chailakhyan MKh, Khryanin VN (1980) Hormonal regulation of sex expression in plants. In: Skoog F (ed) Plant growth substances. Springer, Berlin Heidelberg New York, pp 331–344

  8. Chen CC (1969) The somatic chromosomes of maize. Can J Genet Cytol 11:752–754

  9. Cheng PC, Greyson RI, Waiden DB (1983) Organ initiation and development of unisexual flowers in the tassel and ear of Zea mays. Am J Bot 70:450–462

  10. Coe EH, jr, Neuffer MG (1977) The genetics of corn. In: Sprague GF (ed) Corn and corn improvement. American Society of Agronomy, Madison, Wis, pp 111–223

  11. DeMason DA, Stolte KW, Tisserat B (1982) Floral development in Phoenix dactylifera. Can J Bot 60:1439–1446

  12. Dauphin-Guerin B, Teller G, Durand B (1980) Different endogenous cytokinins between male and female Mercurialis annua L. Planta 148:124–129

  13. Guern J (1987) Regulation from within: the hormone dilemma. Ann Bot (London) 60:75–102

  14. Hansen DJ, Bellman SK, Sacher RM (1976) Gibberellic acidcontrolled sex expression of corn tassels. Crop Sci 16:371–374

  15. Heslop-Harrison J (1961) The experimental control of sexuality and inflorescence structure in Zea mays L. Proc Linn Soc London 172:108–123

  16. Kiesselbach TA (1949) The structure and reproduction of corn. Univ Neb Coll Agric Res Bull 161

  17. Krishnamoorthy HN, Talukdar AR (1979) Effect of gibberellic acid and auxins on growth, flowering and sex expression of Zea mays L. Acta Bot Ind 7:192–195

  18. Libbenga KR, Mennes AM (1987) Hormone binding and its role in hormone action. In: Davis PJ (ed) Plant hormones and their role in plant growth and development. Nijhoff, Boston, pp 194–221

  19. Moss GI, Heslop-Harrison J (1968) Photoperiod and pollen sterility in maize. Ann Bot (London) 32:833–846

  20. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–496

  21. Nickerson NH (1959) Sustained treatment with gibberellic acid of five different kinds of maize. Ann Mo Bot Gard 46:19–37

  22. Pharis RP, King RW (1985) Gibberellins and reproductive development in seed plants. Annu Rev Plant Physiol 36:517–568

  23. Rood SB, Pharis RP, Major DJ (1980) Changes of endogenous gibberellin-like substances with sex reversal of the apical inflorescence of corn. Plant Physiol 66:793–796

  24. Shininger TL (1975) The morphological, anatomical and cytological effects of gibberellins. In: Krishnamoorthy HN (ed) Gibberellins and plant growth. Wiley, New York, pp 203–224

  25. Sladky Z (1969) Role of growth regulators in differentiation processes of maize (Zea mays L.) organs. Biol Plant 11:208–215

  26. Sladky Z (1986) The role of growth regulators in the differentiation of flowers and inflorescences. Biol Plant 28:31–37

  27. Stoddart JH (1986) Gibberellin receptors. In: Chadwick CM, Garrod DR (eds) Hormones, receptors and cellular interactions in plants. Cambridge University Press, Cambridge, pp 91–114

  28. Torrey JG, Fosket DE (1970) Cell division in relation to cytodifferentiation in cultured pea root segments. Am J Bot 57:1072–1080

  29. Trewavas A, Cleland RE (1983) Is plant development regulated by changes in the concentration of growth substances or by changes in the sensitivity to growth substances? Trends Biol Sci 8:354–357

  30. Venis M (1985) Hormone binding sites in plants. Longman, New York

  31. White PR (1943) A handbook of plant tissue culture, 2nd edn. Cattell Press, Lancaster, Pa

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Correspondence to V. R. Bommineni.

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Bommineni, V.R., Greyson, R.I. Regulation of flower development in cultured ears of maize (Zea mays L.). Sexual Plant Reprod 3, 109–115 (1990). https://doi.org/10.1007/BF00198854

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Key words

  • Zea mays
  • Ear initials
  • Kinetin
  • Gibberellic acid
  • Male and female flowers
  • Development