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The pattern of histone H4 expression in the tomato shoot apex changes during development

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Abstract

Two histone H4 cDNA clones were isolated from a tomato (Lycopersicon esculentum Mill.) shoot-tip cDNA library using a heterologous probe from barley (Hordeum vulgare L.). Both cDNAs, which are 81% identical in the coding region, are polyadenylated and belong to a small gene family in the tomato genome. Histone H4 message is abundant in young tissues and rare in older tissues. In the shoot apical meristem, the distribution of H4-expressing cells changes during development. In a juvenile vegetative apex, H4 message is detectable in the central region and the peripheral parts of the meristem. In a mature vegetative apical meristem, H4-expressing cells are localized in the peripheral zone extending into the provascular strands and the rib meristem whereas the central zone is almost devoid of H4 mRNA. After floral transition, H4 mRNA is found throughout the floral meristem, indicating a second change in the pattern of H4 expression. The observed changes in H4 expression are indicative of changes in the distribution of mitotic activity in the shoot apical meristem during plant development. In addition, H4-expressing cells were found to occur frequently in clusters, which may indicate a partial synchronization of cell divisions in the shoot apex.

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Abbreviations

H2A:

histone H2A

H4:

histone H4

SAM:

shoot apical meristem

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Additional information

We thank Drs. Sarah Grant and Francesco Salamini (MPI f. Züchtungsforschung, Köln, FRG) as well as Gregor Schmitz and Peter Starlinger (Institut für Genetik, Universität zu Köln, FRG) for critical reading of the manuscript. This work was supported by the Bundesministerium für Forschung und Technologie.

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Brandstädter, J., Roßbach, C. & Theres, K. The pattern of histone H4 expression in the tomato shoot apex changes during development. Planta 192, 69–74 (1993). https://doi.org/10.1007/BF00198694

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Key words

  • Cell division
  • Histone H4
  • In situ hybridization
  • Lycopersicon esculentum
  • Shoot apical meristem