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Gene expression during induction of somatic embryogenesis in carrot cell suspensions

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Abstract

We report the identification, via their cDNAs, of genes which are temporarily transcribed during the initiation of somatic embryogenesis in carrot (Daucus carota L.) cells cultured in an auxin-free medium. Their expression is roughly associated with the first morphogenetic, or globular, stage. A cDNA library (λ gt 10) was established using poly(A)+ -rich RNAs from cells deprived of auxin for 8 d. By differential screening a number of clones corresponding to early-induced embryogenic genes were identified. For several a temporary accumulation of the specific mRNA between 6 and 16 d after induction was observed. With regard to the nucleotide sequence and the respective deduced amino-acid sequence, two glycine-rich proteins and a polypeptide with a proline-rich domain were among the products of genes activated at the onset of somatic embryogenesis.

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Abbreviations

b, bp:

bases, basepairs

2,4-D:

2,4-dichlorophenoxyacetic acid

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Author information

Correspondence to G. Richter.

Additional information

Sequence data reported here will appear in the EMBL Genbank and DDBJ Nucleotide Sequence Databases under the following accession numbers: X 15436 for clone DC 2.15 (proline-rich protein), X 15706 for clone DC 7.1 (glycine-rich protein, DCGRP) and X 14067 for clone DC 9.1 (glycine-rich protein, DCGRP)

This research was supported by the Deutsche Forschungsgemeinschaft. We thank Mrs. I. Liebscher for her competent assistance.

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Aleith, F., Richter, G. Gene expression during induction of somatic embryogenesis in carrot cell suspensions. Planta 183, 17–24 (1991). https://doi.org/10.1007/BF00197562

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Key words

  • Auxin (gene expression in somatic embryogenesis)
  • Daucus (somatic embryogenesis)
  • Embryogenesis (somatic)
  • Gene expression
  • Protein in somatic embryogenesis
  • Transcription (nuclear genes)