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Different immunoreactivity of endothelial markers in well and poorly differentiated areas of angiosarcomas

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Abstract

This study evaluated the immunohistochemical staining of four endothelial cell markers in well differentiated and poorly differentiated areas of angiosarcomas. Formaldehyde-fixed, paraffin-embedded sections from eight angiosarcomas were studied using the antibodies anti-factor VIII-related antigen (FVIII-RA), Ulex europaeus I agglutinin, anti-CD34 (QBEND/10) and anti-CD31 (JC70). The immunostaining of the angiomatous (well differentiated) and solid (poorly differentiated) areas was separately analysed and specificity was evaluated in 20 non-vascular tumours. The antibody anti-CD31 and Ulex europaeus were the most sensitive markers staining well differentiated vasoformative structures and poorly differentiated solid areas. Anti-FVIII-RA and anti-CD34 did not stain undifferentiated malignant endothelial cells from solid areas. Ulex europaeus and anti-CD34 showed very low specificity; in contrast, none of the non-vascular tumours expressed CD31 or FVIII-RA. JC70 (anti-CD31) appears to be the most useful marker in elucidating the vascular nature of angiosarcomas. Is important to emphasize the lack of specificity of Ulex europaeus and the low sensitivity of anti-CD34 and anti-FVIII-RA for poorly differentiated lesions.

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References

  1. 1.

    Aiba S, Tabata N, Ishii H, Ootani H, Tagami H (1992) Dermatofibrosarcoma protuberans is a unique fibrohistiocytic tumour expressing CD34. Br J Dermatol 127:79–84

  2. 2.

    Alles JU, Bosslet K (1988) Immunocytochemistry of angiosarcomas. A study of 19 cases with special emphasis on the applicability of endothelial cell specific markers to routinely prepared tissues. Am J Clin Pathol 89:463–471

  3. 3.

    Andrews RG, Singer JW, Bernstein ID (1986) Monoclonal antibody 12.8 recognizes a 115 kd molecule present on both unipotent and multipotent hematopoietic colony forming cells and their precursors. Blood 67:842–845

  4. 4.

    Borowitz MJ, Gockerman JP, Moore JO, Civin CI, Page SO, Robertson J, Bigner SH (1989) Clinicopathologic and cytogenetic features of CD34 (My-10)-positive acute nonlymphocytic leukemia. Am J Clin Pathol 91:265–270

  5. 5.

    Burgdorf WHC, Mukai K, Rosai J (1981) Immunohistochemical identification of factor VIII-related antigen in endothelial cells of cutaneous lesions of alleged vascular nature. Am J Clin Pathol 75:167–171

  6. 6.

    Civin CI, Strauss LC, Brovall C, Fackler MJ, Schwartz JF, Shaper JH (1984) Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by monoclonal antibody raised against KG-1a cells. J Immunol 133:157–165

  7. 7.

    Cohen PR, Rapini RP, Farhood AI (1993) Expression of the human hematopoietic progenitor cell antigen CD34 in vascular and spindle cell tumors. J Cutan Pathol 20:15–20

  8. 8.

    DeYoung BR, Wick MR, Fitzgibbon JF, Sirgi KE, Swanson PE (1993) CD31. An immunospecific marker for endothelial differentiation in human neoplasms. Appl Immunohistochem 1:97–100

  9. 9.

    DeYoung BR, Swanson PE, Argenyi ZB, Ritter JH, Fitsgibbon JF, Stahl DJ, Hoover W, Wick MR (1995) CD31 immunoreactivity in mesenchymal neoplasms of the skin and subcutis. J Cutan Pathol 22:215–222

  10. 10.

    Fina L, Molgaard HV, Robertson D, Bradley NJ, Monaghan P, Delia D, Sutherland BR, Baker MA, Greaves MF (1990) Expression of the CD34 gene in vascular endothelial cells. Blood 75:2417–2426

  11. 11.

    Fletcher C, Ramani P (1990) QBEND/10: a useful but by no means specific marker of Kaposi's sarcoma. J Pathol 162:273–277

  12. 12.

    Folkman J, Haudenschild CC, Zetter BR (1979) Long-term culture of capillary endothelial cells. Proc Natl Acad Sci USA 76:5217–5221

  13. 13.

    Gimbrone MA, Cotran RS, Folkman J (1974) Human vascular endothelial cells in culture. J Cell Biol 60:673–684

  14. 14.

    Hashimoto K, Fujiwara K, Mehregan A (1993) Current topics of immunohistochemistry as applied to skin tumors. J Dermatol 20:521–532

  15. 15.

    Heng MCY, Fallon-Friedlander S, Benett R (1992) Expression of Ulex europaeus agglutinin I lectin-binding sites in squamous cell carcinomas and their abscence in basal cell carcinomas. Am J Dermatopathol 14:216–219

  16. 16.

    Holthoffer H, Virtanen I, Kariniemi A-L, Hormia M, Linder E, Miettinen A (1982) Ulex europaeus I lectin as a marker for vascular endothelium in human tissues. Lab Invest 47:60–66

  17. 17.

    Jaffe EA, Hoyer LW, Nachman RL (1973) Synthesis of antihemophilic factor antigen by cultured endothelial cells. J Clin Invest 52:2757–2764

  18. 18.

    Koutts J, Howard MA, Firkin BG (1974) Factor VIII physiology and pathology in man. Semin Hematol 11:115–145

  19. 19.

    Kraffert C, Planas L, Penneys NS (1991) Kaposi's sarcoma: immunohistologic evidence of a vascular endothelial origin. Arch Dermatol 127:1734–1735

  20. 20.

    Kuzu I, Bicknell R, Harris AL, Jones M, Gatter KC, Mason DY (1992) Heterogeneity of fascular endothelial cells with relevance to diagnosis of vascular tumours. J Clin Pathol 45:143–148

  21. 21.

    Lansdorp PM, Sutherland HJ, Eaves CJ (1990) Selective expression of CD45 isoforms in functional subpopulations of CD34+ hematopoietic cells from human bone marrow. J Exp Med 172:363–366

  22. 22.

    Leader M, Collins M, Patel J, Henry K (1986) Staining of Factor VIII-related antigen and Ulex europaeus agglutinin (UEAI) in 230 tumours. An assessment of their specificity for angiosarcoma and Kaposi's sarcoma. Histopathology 10:1153–1162

  23. 23.

    Loken MR, Shah VO, Dattilio KL, Civin CI (1987) Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood 70:1316–1324

  24. 24.

    Miettinen M, Holthofer H, Lehto V-P, Miettinen A, Virtanen I (1983) Ulex europaeus I lectin as a marker for tumours derived from endothelial cells. Am J Clin Pathol 79:32–36

  25. 25.

    Miettinen M, Lindenmayer AE, Chaubal A (1994) Endothelial cell markers CD31, CD34, and BNH9 antibody to H- and Y-antigens evaluation of their specificity and sensitivity in the diagnosis of vascular tumors and comparison with Von Willebrand factor. Mod Pathol 7:82–90

  26. 26.

    Mukai K, Rosai J, Burgdorf WHC (1980) Localization of factor VIII-related antigen in vascular endothelial cells using an immunoperoxidase method. Am J Surg Pathol 4:273–276

  27. 27.

    Newman PJ, Berndt MC, Gorski J, White GC, Lyman S, Paddock C, Muller WA (1990) PECAM-1 (CD31) cloning and relation to adhesion molecules of the immunoglobulin gene superfamily. Science 247:1219–1222

  28. 28.

    Nickoloff BJ (1991) The human progenitor cell antigen (CD34) is localized on endothelial cells, dermal dendritic cells, and perifollicular cells in formalin fixed normal skin, and on proliferating endothelial cells and stromal spindle-shaped cells in Kaposi's sarcoma. Arch Dermatol 127:523–529

  29. 29.

    Ordoñez NG, Batsakis JG (1984) Comparison of Ulex europaeus I lectin and factor VIII-related antigen in vascular lesions. Arch Pathol Lab Med 108:129–132

  30. 30.

    Parums DV, Cordell JL, Micklem K, Heryet AR, Gatter KC, Mason DY (1990) JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections. J Clin Pathol 43:752–757

  31. 31.

    Poblet E, Jimenez-Acosta F (1992) QBEND/10 (anti CD34): an immunohistochemical marker of hair tumors derived from the external root sheath (abstract). J Cutan Pathol 9:543

  32. 32.

    Poblet E, Jimenez-Acosta F, Rocamora A (1994) QBEND/10 (anti-CD34 antibody) in external root sheath cells and hair tumors. J Cutan Pathol 21:224–228

  33. 33.

    Ramani P, Bradley NJ, Fletcher CDM (1990) QBEND/10, a new monoclonal antibody to endothelium: assesment of its diagnostic utility in paraffin sections. Histopathology 17:237–242

  34. 34.

    Sankey EA, More L, Dhillon AP (1990) QBEND/10: a new immunostain for the routine diagnosis of Kaposis's sarcoma. J Pathol 161:267–271

  35. 35.

    Schlingemann RO, Rietveld FJR, DeWall RMW, Bradley NJ, Skene AI, Davies AJS, Greaves MF, Denekamp J, Ruiter DJ (1990) Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma. Lab Invest 62:690–696

  36. 36.

    Sehested M, Hou-Jensen K (1981) Factor VIII-related antigen as an endothelial cell marker in benign and malignant diseases. Virchows Arch [A] 391:217–225

  37. 37.

    Sirgi KE, Wick MR, Swanson PE (1993) B72.3 and CD34 immunoreactivity in malignant epithelioid soft tissue tumors. Adjuncts in the recognition of endothelial neoplasms. Am J Surg Pathol 17:179–185

  38. 38.

    Suster S, Wong TY (1994) On the discriminatory value of anti-HPCA-1 (CD34) in the differential diagnosis of benign and malignant cutaneous vascular proliferations. Am J Dermatopathol 16:355–363

  39. 39.

    Traweek ST, Kandalaft PL, Mehta P, Battifora H (1991) The human hematopoietic progenitor cell antigen (CD34) in vascular neoplasia. Am J Clin Pathol 96:25–31

  40. 40.

    Warhol MJ, Sweet JM (1984) The ultrastructural localization of von Willebrand factor in endothelial cells. Am J Pathol 117:310–315

  41. 41.

    Watt SM, Karhi K, Gatter K, Furley AJW, Katz FE, Healy LE, Altass LJ, Bradley LJ, Sutherland DR, Levinsky R, Greaves MF (1987) Distribution and epitope analysis of the cell membrane glycoprotein (HPCA-1) associated with human hemopoietic progenitor cells. Leukemia 1:417–426

  42. 42.

    Wilson AJ (1984) Factor VIII-related antigen staining by immunoperoxidase techniques in smaller laboratories. Am J Clin Pathol 81:117–120

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Correspondence to F. J. Jimenez.

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Poblet, E., Gonzalez-Palacios, F. & Jimenez, F.J. Different immunoreactivity of endothelial markers in well and poorly differentiated areas of angiosarcomas. Vichows Archiv A Pathol Anat 428, 217–221 (1996). https://doi.org/10.1007/BF00196693

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Key words

  • Immunohistochemistry
  • Angiosarcoma
  • CD31
  • CD34
  • Factor VIII-related antigen