Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

DNA-ploidy, G2M-fractions and prognosis of stages B and C prostate carcinoma

  • 18 Accesses

  • 4 Citations

Abstract

Paraffin embedded tissue of 49 stage C and 27 stage B prostate adenocarcinomas was investigated by flow cytometry. All patients were treated by radical prostatectomy with pelvic lymphadenectomy and followed up for 5–10 years. The tumour was separated from the benign tissue to increase the proportion of tumour cells. Ten stage C and seven stage B carcinomas had to be excluded because of poor fixation. Six of the 39 (15%) stage C and 1/20 (5%) stage B carcinomas were aneuploid. Cell cycle analysis was done with correction for sliced nuclei and background subtraction. The threshold between carcinomas with low and with increased (“tetraploid”) G2M-fraction was determined by comparing carcinomas with and without tumour progression. Sixty-seven percent of the patients with non-euploid stage C carcinomas and 11% of those with euploid carcinomas suffered from tumour progression (P<0.01). The respective values for the stage B carcinomas were 67% and 6% (P<0.01). These results demonstrate the strong prognostic impact of DNA-ploidy and G2M-fractions for each individual patient.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Alanen KA, Joensuu H, Klemi PJ (1989) Autolysis is a potential cause of false aneuploid peaks in flow cytometry DNA histograms. Cytometry 10:417–425

  2. 2.

    Böcking A, Chatelain R, Orthen U, Gien G, Kalckreuth G von, Jocham D, Wohltmann D (1988) DNA-grading of prostatic carcinoma: prognostic validity and reproducibility. Anticancer Res 8:129–135

  3. 3.

    Brothman AR, Lesho LJ, Somers KD, Schellhammer PF, Ladage LE, Merchant DJ (1989) Cytogenetic analysis of four primary prostatic cultures. Cancer Genet Cytogenet 37:241–248

  4. 4.

    Camplejohn RS, Macartney JC (1986) Comments of “effects of section thickness on quality of flow cytometric DNA content determinantion in paraffin-embedded tissues”. Cytometry 7:612–615

  5. 5.

    Dean PN (1985) Methods of data analysis in flow cytometry. In: Dilla MA van, Dean PN, Laerum OD, Melamed MR (eds) Flow cytometry: instrumentation and data analysis. Academic Press, pp 195–221

  6. 6.

    Deitch AD, Stand MA, White RW deVere (1989) Deoxyribonucleic acid flow cytometry of benign prostatic disease. J Urol 142:759–762

  7. 7.

    Dejter SW, Cunningham RE, Noguchi PD, Jones RV, Moul JW, McLeaod DG, Lynch JH (1989) Prognostic significance of DNA ploidy in carcinoma of prostate. Urology 33:361–366

  8. 8.

    Feichter GE, Goerttler K (1986) Pitfalls in the preparation of nuclear suspensions from paraffin-embedded tissue for flow cytometry. Cytometry 7:616–619

  9. 9.

    Fordham MVP, Burdge AH, Matthews J, Williams G, Cooke T (1986) Prostatic carcinoma cell DNA content measured by flow cytometry and its relation to clinical outcome. Br J Surg 73:400–403

  10. 10.

    Forsslund G, Zetterberg A (1990) Ploidy level determinations in high-grade and low-grade malignant variants of prostatic carcinoma. Cancer Res 50:4281–4285

  11. 11.

    Frankfurt OS, Chin JL, Englander LS, Greco WR, Pontes JE, Rustum YM (1985) Relationship between DNA ploidy, glandular differentiation and tumor spread in human prostate cancer. Cancer Res 45:1418–1423

  12. 12.

    Frohmüller H, Theiβ M, Wirth MP (1991) Radical prostatectomy for carcinoma of the prostate — long term follow up of 115 patients. Eur J Urol 19:279–283

  13. 13.

    Hedley DW, Friedlander ML, Taylor IW, Rugg CA, Musgrove EA (1983) Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem 31:1333–1335

  14. 14.

    Hiddemann W, Schumann J, Andreeff M, Barlogie B, Herman CJ, Reif RC, Mayall BH, Murphy RF, Sandberg AA (1984) Convention on nomenclature for DNA cytometry. Cytometry 5:445–446

  15. 15.

    Joensuu H, Kallioniemi O-P (1989) Different opinions on classification of DNA histograms produced from paraffin-embedded tissue. Cytometry 10:711–717

  16. 16.

    Kallioniemi OP, Visakorpi T, Holli K, Heikkinen A, Isola J, Koivula T (1991) Improved prognostic impact of S-phase values from paraffin-embedded breast and prostate carcinomas after correcting for nuclear slicing. Cytometry 12:413–421

  17. 17.

    Klein FA, Ratliff JE, White FK (1988) DNA distribution patterns of prostatic tissue obtained at time of transurethral resection. Urology 31:260–265

  18. 18.

    Lee SE, Currin SM, Paulson DF, Walther PJ (1988) Flow cytometric determination of ploidy in prostatic adenocarcinoma: a comparison with seminal vesicle involvement and histopathological grading as a predictor of clinical recurrence. J Urol 140:769–774

  19. 19.

    Limon J, Lundgren R, Elfving P, Heim S, Kristoffersson U, Mandahl N, Mitelman F (1989) Double minutes in two primary adenocarcinomas of the prostate. Cancer Genet Cytogenet 39:191–194

  20. 20.

    Lundberg S, Carstensen J, Rundquist I (1987) DNA flow cytometry and histopathological grading of paraffin-embedded prostate biopsy specimens in a survival study. Cancer Res 47:1973–1977

  21. 21.

    Malinin TI, Hornicek FJ, Block NL, Malinin GI (1988) Aneuploidy of glandular epithelial cells in histological normal prostate glands. Experientia 44:247–249

  22. 22.

    McIntire TL, Murphy WM, Coon JS, Chandler RW, Schartz D, Conway S, Weinstein RS (1988) The prognostic value of DNA ploidy combined with histologic substaging for incidental carcinoma of the prostate gland. Am J Clin Pathol 89:370–373

  23. 23.

    Miller J, Horsfall DJ, Marshall VR, Rao DM, Leong SY (1991) The prognostic value of deoxyribonucleic acid flow cytometric analysis in stage D2 prostatic carcinoma. J Urol 145:1192–6

  24. 24.

    Montgomery BT, Nativ O, Blute ML, Farrow GM, Myers RP, Zincke H, Therneau TM, Lieber MM (1990) Stage B prostate adenocarcinoma. Flow cytometric nuclear DNA ploidy analysis. Arch Surg 125:327–331

  25. 25.

    Müller H-A, Altenähr E, Böcking A, Dhom G, Faul P, Göttinger H, Helpap B, Hohbach Ch, Kastendieck H, Leistenschneider W (1980) Über Klassifikation und Grading des Prostatakarzinoms. Verh Dtsch Ges Pathol 64:609–611

  26. 26.

    Nativ O, Winkler HZ, Raz Y, Therneau TM, Farrow GM, Myers RP, Zincke H, Lieber MM (1989) Stage C prostatic adenocarcinoma: flow cytometric nuclear DNA ploidy analysis. Mayo Clin Proc 64:911–919

  27. 27.

    Neill WA, Norval M, Habib FK (1989) Nuclear DNA analysis of prostate tissues: correlation with stage and grade of tumour. Urol Int 44:141–146

  28. 28.

    Schellhammer PF (1988) Radical prostatectomy. Patterns of local failure and survival in 67 patients. Urology 31:191–197

  29. 29.

    Schultz RE, Varello MA, Tsou KC, Wein AJ, Murphy JJ (1985) Simultaneous flow cytometric deoxyribonucleic acid and acid phosphatase analysis of benign and malignant lesions of the prostate. J Urol 134:1133–1136

  30. 30.

    Seppelt U, Sprenger E (1984) Nuclear DNA cytophotometry in prostate carcinoma. Cytometry 5:258–262

  31. 31.

    Shackney SE, Burholt DR, Pollice AA, Smith CA, Pugh RP, Hartsock RJ (1990) Discrepancies between flow cytometric and cytogenetic studies in the detection of aneuploidy in human solid tumors. Cytometry 11:94–104

  32. 32.

    Stephenson RA, Gay H, Fair WR, Melamed MR (1986) Effect of section thickness on quality of flow cytometric DNA content determinations in paraffin-embedded tissues. Cytometry 7:41–43

  33. 33.

    Stephenson RA, James BC, Gay H, Fair WR, Whitemore WF, Melamed MR (1987) Flow cytometry of prostate cancer: relationship of DNA content to survival. Cancer Res 47:2504–2507

  34. 34.

    Tavares AS, Costa J, De Carvalho A, Reis M (1966) Tumour ploidy and prognosis in carcinomas of the bladder and prostate. Br J Cancer 20:438–441

  35. 35.

    Tribukait B, Ronstrom L, Espositi P (1983) Quantitative and qualitative aspects of flow DNA measurements related to the cytologic grade in prostatic carcinoma. Anal Quant Cytol Histol 5:107–111

  36. 36.

    Winkler HZ, Rainwater LM, Myers RP, Farrow GM, Therneau TM, Zincke H, Lieber MM (1988) Stage D1 prostatic adenocarcinoma: significance of nuclear DNA ploidy patterns studied by flow cytometry. Mayo Clin Proc 63:103–112

  37. 37.

    Wirth MP, Müller HA, Manseck A, Müller J, Frohmüller HGW (1991) Value of nuclear DNA ploidy patterns in patients with prostate cancer after radical prostatectomy. Eur Urol 20:248–252

Download references

Author information

Correspondence to J. G. Müller.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Müller, J.G., Demel, S., Müller, H.A. et al. DNA-ploidy, G2M-fractions and prognosis of stages B and C prostate carcinoma. Vichows Archiv A Pathol Anat 424, 647–651 (1994). https://doi.org/10.1007/BF00195780

Download citation

Key words

  • Prostate carcinoma
  • Radical prostatectomy
  • Flow cytometry
  • DNA-ploidy
  • Prognosis