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A cultured human oligodendroglioma cell line and herpes simplex virus-infected cells share antigenic determinants

Abstract

Cell cultures derived from 60 different human brain tumors were screened for the presence of HSV infected cell antigens by indirect immunofluorescence using a polyclonal rabbit antiserum reacting with herpes simplex virus (HSV), 3 monoclonal antibodies recognising different HSV specified proteins, and one monoclonal antibody TI81 reacting with a DNA binding protein present in HSV-infected cells. Only one tumor (IN/157), derived from an oligodendroglioma, stained with the polyclonal antiserum. TI81 but none of the other monoclonal antibodies used also specifically reacted with IN/157 cells. High levels of the TI81-defined protein were detected using immunoblotting in HSV-1 infected BHK/21 cells but not in IN/157 cells. T181 may react with either an epitope shared between two different molecules in HSV I infected and IN/157 cells or a cell-specified polypeptide that is upregulated after HSV-1 infection.

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References

  1. 1

    Bullard DE, Bigner DD: Animal models and virus induction of tumours. In: Thomas DGT and Graham DT (eds) Brain tumours, scientific basis, clinical investigation and current therapy. Butterworths, London, 1984 pp 51–84.

  2. 2

    Baringer JR: Herpes simplex virus infection of nervous tissue in animals and man. Prog Med Virol 20:1–16, 1975.

  3. 3

    Nahmias AJ, Roizman B: Infection with herpes simplex viruses 1 and 2. New Engl J Med 289:719–725, 1973.

  4. 4

    Weiss RA: Viruses and human cancer. In: Mahy BWJ and Pattison JR (eds), The Microbe. SGM Symposium, Cambridge University Press 1984, pp 211–240.

  5. 5

    Zur Hausen H: Human genital cancer: Synergism between two virus infections or synergism between a virus infection and initiating events. Lancet (ii), 1370–1372, 1982.

  6. 6

    Galloway DA, McDougall JK: The oncogenic potential of herpes simplex viruses: evidence for a ‘hit and run’ mechanism. Nature, 302:21–24, 1983.

  7. 7

    Park M, Kitchener H, Macnab JCM: Detection of herpes simplex virus type 2 DNA restriction fragments in human cervical carcinoma tissue. EMBO Journal 2:1029–1034, 1983.

  8. 8

    Park M, Macnab JCM: Induction of a latent herpes simplex virus from a rat tumour initiated by herpes simplex virus-transformed cells. J gen Virol 64:755–758, 1983.

  9. 9

    Morgan D, Freshney RK, Darling JL, Thomas DGT, Celik R: Assay of anti-cancer drugs in tissue culture: Cell cultures of biopsies from human astrocytoma. Brit J Cancer 47:205–214, 1983.

  10. 10

    Macpherson I, Stoker MGP: Polyoma transformation of hamster cell clones — an investigation of genetic factors affecting cell competence. Virology 16:147–151, 1962.

  11. 11

    Kennedy PGE, Clements GB, Brown SM: Differential susceptibility of human neural cell types in culture to infection with herpes simplex virus (HSV). Brain 106:101–119, 1983.

  12. 12

    La Thangue NB, Chan WL: The characterization and purification of DNA binding proteins present within herpes simplex virus infected cells using monoclonal antibodies. Arch Virol 79:13–33, 1984.

  13. 13

    Eisenbarth GS, Walsh FS, Nirenberg M: Monoclonal antibody to a plasma membrane antigen of neurons. Proc Nat Acad Sci (Wash) 76:4913–4917, 1979.

  14. 14

    Raff MC, Miller RT, Noble M: A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending upon culture medium. Nature 33:390–396, 1983.

  15. 15

    Kennedy PGE, Lisak RP, Raff MC: Cell type-specific markers for human glial and neuronal cells in culture. Lab Invest 43:342–351, 1980.

  16. 16

    La Thangue NB, Shriver K, Dawson C, Chan WL: Herpes simplex virus infection causes the accumulation of a heatshock protein. EMBO J 3:267–277, 1984.

  17. 17

    Timbury MC: Temperature-sensitive mutants of herpes simplex virus type 2. J gen Virol 13:373–376, 1971.

  18. 18

    Halliburton IW, Timbury MC: Characterization of temperature-sensitive mutants of herpes simplex virus type 2 Growth and DNA synthesis. Virology 54:60–68, 1973.

  19. 19

    Brown SM, Subak-Sharpe JH, Warren KG, Wroblewska Z, Koprowski H: Detection by complementation of defective or uninducible (herpes simplex type 1) virus genomes latent in human ganlia. Proc Nat Acad Sci (Wash) 76:2364–2368, 1979.

  20. 20

    Kennedy PGE, Watkins B, Thomas DGT, Noble MD: Antigenic expression by cells derived from human gliomas does not correlate with morphological classification. Submitted for publication.

  21. 21

    Lewis ME, Brown SM, Warren K, Subak-Sharpe JH: Recovery of herpes simplex virus genetic information from human trigeminal ganglion cells following superinfection with herpes simplex virus type 2 temperature-sensitive mutants. J gen Virol 65:215–219, 1984.

  22. 22

    Walsh FS: Identification and characterization of plasma membrane components of neurons and muscle cells using monoclonal antibodies. In: Brzin M, Sket D and Bachelard H, (eds) Synaptic constituents in health and disease. Pergamon Press, Oxford, 1980, pp. 285–320.

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Correspondence to P. G. E. Kennedy.

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Kennedy, P.G.E., Watkins, B.A., LaThangue, N.B. et al. A cultured human oligodendroglioma cell line and herpes simplex virus-infected cells share antigenic determinants. J Neuro-Oncol 4, 389–396 (1987). https://doi.org/10.1007/BF00195610

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Keywords

  • oligodendroglioma
  • herpes simplex virus
  • monoclonal antibody