Cell cultures derived from 60 different human brain tumors were screened for the presence of HSV infected cell antigens by indirect immunofluorescence using a polyclonal rabbit antiserum reacting with herpes simplex virus (HSV), 3 monoclonal antibodies recognising different HSV specified proteins, and one monoclonal antibody TI81 reacting with a DNA binding protein present in HSV-infected cells. Only one tumor (IN/157), derived from an oligodendroglioma, stained with the polyclonal antiserum. TI81 but none of the other monoclonal antibodies used also specifically reacted with IN/157 cells. High levels of the TI81-defined protein were detected using immunoblotting in HSV-1 infected BHK/21 cells but not in IN/157 cells. T181 may react with either an epitope shared between two different molecules in HSV I infected and IN/157 cells or a cell-specified polypeptide that is upregulated after HSV-1 infection.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Bullard DE, Bigner DD: Animal models and virus induction of tumours. In: Thomas DGT and Graham DT (eds) Brain tumours, scientific basis, clinical investigation and current therapy. Butterworths, London, 1984 pp 51–84.
Baringer JR: Herpes simplex virus infection of nervous tissue in animals and man. Prog Med Virol 20:1–16, 1975.
Nahmias AJ, Roizman B: Infection with herpes simplex viruses 1 and 2. New Engl J Med 289:719–725, 1973.
Weiss RA: Viruses and human cancer. In: Mahy BWJ and Pattison JR (eds), The Microbe. SGM Symposium, Cambridge University Press 1984, pp 211–240.
Zur Hausen H: Human genital cancer: Synergism between two virus infections or synergism between a virus infection and initiating events. Lancet (ii), 1370–1372, 1982.
Galloway DA, McDougall JK: The oncogenic potential of herpes simplex viruses: evidence for a ‘hit and run’ mechanism. Nature, 302:21–24, 1983.
Park M, Kitchener H, Macnab JCM: Detection of herpes simplex virus type 2 DNA restriction fragments in human cervical carcinoma tissue. EMBO Journal 2:1029–1034, 1983.
Park M, Macnab JCM: Induction of a latent herpes simplex virus from a rat tumour initiated by herpes simplex virus-transformed cells. J gen Virol 64:755–758, 1983.
Morgan D, Freshney RK, Darling JL, Thomas DGT, Celik R: Assay of anti-cancer drugs in tissue culture: Cell cultures of biopsies from human astrocytoma. Brit J Cancer 47:205–214, 1983.
Macpherson I, Stoker MGP: Polyoma transformation of hamster cell clones — an investigation of genetic factors affecting cell competence. Virology 16:147–151, 1962.
Kennedy PGE, Clements GB, Brown SM: Differential susceptibility of human neural cell types in culture to infection with herpes simplex virus (HSV). Brain 106:101–119, 1983.
La Thangue NB, Chan WL: The characterization and purification of DNA binding proteins present within herpes simplex virus infected cells using monoclonal antibodies. Arch Virol 79:13–33, 1984.
Eisenbarth GS, Walsh FS, Nirenberg M: Monoclonal antibody to a plasma membrane antigen of neurons. Proc Nat Acad Sci (Wash) 76:4913–4917, 1979.
Raff MC, Miller RT, Noble M: A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending upon culture medium. Nature 33:390–396, 1983.
Kennedy PGE, Lisak RP, Raff MC: Cell type-specific markers for human glial and neuronal cells in culture. Lab Invest 43:342–351, 1980.
La Thangue NB, Shriver K, Dawson C, Chan WL: Herpes simplex virus infection causes the accumulation of a heatshock protein. EMBO J 3:267–277, 1984.
Timbury MC: Temperature-sensitive mutants of herpes simplex virus type 2. J gen Virol 13:373–376, 1971.
Halliburton IW, Timbury MC: Characterization of temperature-sensitive mutants of herpes simplex virus type 2 Growth and DNA synthesis. Virology 54:60–68, 1973.
Brown SM, Subak-Sharpe JH, Warren KG, Wroblewska Z, Koprowski H: Detection by complementation of defective or uninducible (herpes simplex type 1) virus genomes latent in human ganlia. Proc Nat Acad Sci (Wash) 76:2364–2368, 1979.
Kennedy PGE, Watkins B, Thomas DGT, Noble MD: Antigenic expression by cells derived from human gliomas does not correlate with morphological classification. Submitted for publication.
Lewis ME, Brown SM, Warren K, Subak-Sharpe JH: Recovery of herpes simplex virus genetic information from human trigeminal ganglion cells following superinfection with herpes simplex virus type 2 temperature-sensitive mutants. J gen Virol 65:215–219, 1984.
Walsh FS: Identification and characterization of plasma membrane components of neurons and muscle cells using monoclonal antibodies. In: Brzin M, Sket D and Bachelard H, (eds) Synaptic constituents in health and disease. Pergamon Press, Oxford, 1980, pp. 285–320.
About this article
Cite this article
Kennedy, P.G.E., Watkins, B.A., LaThangue, N.B. et al. A cultured human oligodendroglioma cell line and herpes simplex virus-infected cells share antigenic determinants. J Neuro-Oncol 4, 389–396 (1987). https://doi.org/10.1007/BF00195610
- herpes simplex virus
- monoclonal antibody