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A cultured human oligodendroglioma cell line and herpes simplex virus-infected cells share antigenic determinants


Cell cultures derived from 60 different human brain tumors were screened for the presence of HSV infected cell antigens by indirect immunofluorescence using a polyclonal rabbit antiserum reacting with herpes simplex virus (HSV), 3 monoclonal antibodies recognising different HSV specified proteins, and one monoclonal antibody TI81 reacting with a DNA binding protein present in HSV-infected cells. Only one tumor (IN/157), derived from an oligodendroglioma, stained with the polyclonal antiserum. TI81 but none of the other monoclonal antibodies used also specifically reacted with IN/157 cells. High levels of the TI81-defined protein were detected using immunoblotting in HSV-1 infected BHK/21 cells but not in IN/157 cells. T181 may react with either an epitope shared between two different molecules in HSV I infected and IN/157 cells or a cell-specified polypeptide that is upregulated after HSV-1 infection.

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Correspondence to P. G. E. Kennedy.

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Kennedy, P.G.E., Watkins, B.A., LaThangue, N.B. et al. A cultured human oligodendroglioma cell line and herpes simplex virus-infected cells share antigenic determinants. J Neuro-Oncol 4, 389–396 (1987).

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  • oligodendroglioma
  • herpes simplex virus
  • monoclonal antibody