Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Antibodies to the second Epstein-Barr virus nuclear antigen in non-Hodgkin lymphomas

  • 12 Accesses


Human antibodies against the first and second Epstein-Barr virus encoded nuclear antigens (EBNA-1 and EBNA-2) were analysed by modified ELISA reaction on immunoblots: 5 out of 20 EBNA-1 positive, healthy donor sera reacted with the EBNA-2. Healthy EBV seronegatives contained no antibodies against EBNA-2. The antibodies against this EBNA-2 developed several months after acute EBV infections. Four out of 8 infectious mononucleosis sera failed to react EBNA-2 in the late stage. In the group of EBV seropositive patients with non-Hodgkin lymphoma, 14 out of 18 sera contained EBNA-2 antibodies.

This is a preview of subscription content, log in to check access.


  1. 1.

    Dillner J, Kallin B, Ehlin-Henriksson B, Timár L, Klein G (1985) Characterization of a second Epstein-Barr virus nuclear antigen associated with the Bam HI WYH region of EBV DNA. Int J Cancer 35:359–366

  2. 2.

    Dumont J, Liabeuf A, Henle W, Feingold M, Kourilsky PM (1976) Anti-EBV antibody titers in non-Hodgkin lymphomas. Int J Cancer 18:14–23

  3. 3.

    Editorial (1985) EBV and persistent malaise. Lancet 1:1017–1018

  4. 4.

    Gergely L, Czeglédy J, Szalka A, Váczi L, Binder L (1977) Epstein-Barr virus and cytomegalovirus antibodies in infectious mononucleosis. Acta Microbiol Hung 24:13–19

  5. 5.

    Gergely L, Sternås L, Dillner J, Klein G (1984) Molecular size variation of EBNA is determined by the EB viral genome. Intervirology 22:85–96

  6. 6.

    Grogan EA, Summers VP, Dowlling S, Shedd D, Gradoville L, Miller G (1983) Two Epstein-Barr viral nuclear neoantigens, distinguished by gene transfer, serology and chromosome binding. Proc Natl Acad Sci USA 80:7650–7653

  7. 7.

    Henessy K, Kieff E (1983) One of two Epstein-Barr virus nuclear antigens contains a glycine-alanine copolymer domain. Proc Natl Acad Sci USA 80:5665–5669

  8. 8.

    Henle W, Henle G, Horwitz CA (1974) Epstein-Barr virus specific diagnostic tests in infectious mononucleosis. Hum Pathol 5:551–565

  9. 9.

    Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685

  10. 10.

    Miller G, Grogan EA, Fischer DK, Niederman JC, Schooley RT, Henle W, Lenoir G, Lin CR (1985) Antibody responses to two Epstein-Barr virus nuclear antigens defined by gene transfer. N Engl J Med 312:750–755

  11. 11.

    Sumaya CV (1985) Serological testing for Epstein-Barr virus — developments in interpretation. J Infect Dis 151:984–987

Download references

Author information

Correspondence to L. Gergely.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Gergely, L., Czeglédy, J., Váczi, L. et al. Antibodies to the second Epstein-Barr virus nuclear antigen in non-Hodgkin lymphomas. Med Microbiol Immunol 176, 175–179 (1987). https://doi.org/10.1007/BF00193899

Download citation


  • Lymphoma
  • Late Stage
  • Healthy Donor
  • Nuclear Antigen
  • Human Antibody