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In vitro development of globular zygotic wheat embryos

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Summary

We have established in vitro culture conditions for globular zygotic wheat embryos (Triticum aestivum L.). Their nutritional requirements have been systematically investigated. The initial sucrose concentration, as well as the sucrose concentration during the culture, a 6-benzylaminopurine supplement, the use of nitrates and ammonium as nitrogen source have a major influence on the embryo development. Proline has an inhibitory effect on the germination. A double layer system with different media was used to give a continuous variation of the medium composition with time. These culture conditions allowed normal direct embryogenesis in up to 47% of the globular embryos.

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Abbreviations

BAP:

6-benzylaminopurine

MES:

2-N-morpholinoethane-sulfonic acid

MS:

Murashige and Skoog (1962)

References

  1. Armstrong CL, Green CE (1985) Planta 164: 207–214

  2. Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Scientia Sinic. 18: 659–668

  3. Holm PB, Knudsen S, Mouritzen P, Negri D, Olsen FL, Roué C (1994) Plant Cell 6: 531–543

  4. Hu C, Wang P (1986) In Evans DA, Sharp WR, Ammirato PV (Eds) Handbook of plant cell culture, Macmillan Publishing Company, New York, vol 4 pp 43–96

  5. Iglesias VA, Gisel A, Potrykus I, Sautter C (1994) Plant Cell Reports 13: 377–380

  6. Liu CM, Xu ZH, Chua NH (1993) Plant Journal 3: 291–300

  7. Lupotto E, Lusardi MC (1988) Maydica XXXIII: 163–177

  8. Kranz E, Lörz H (1993) Plant Cell 5: 739–746

  9. Monnier M (1976) Rev. Cyt. Biol. végét. 39: 1–120

  10. Monnier M (1990) In: Bhojwani SS (Ed) Plant tissue culture: applications and limitations, Elsevier, Amsterdam Oxford New York Tokyo, pp 366–393

  11. Mordhorst AP, Lörz H (1993) J. Plant Physiol. 142: 485–492

  12. Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497

  13. Norstog K (1965) Amer. J. Bot. 52: 538–546

  14. Raghavan V (1976) In Experimental embryogenesis in vascular plants, Academic Press, London New-York San Fransisco, pp 221–255

  15. Rijven AHGC (1958) Aust. J. Biol. Sci. 11: 142–154

  16. Smith JG (1973) Plant Physiol. 51: 454–458

  17. Triplett BA, Quatrano RS (1982) Dev. Biol. 91: 491–496

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Communicated by H. Lörz

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Fischer, C., Neuhaus, G. In vitro development of globular zygotic wheat embryos. Plant Cell Reports 15, 186–191 (1995). https://doi.org/10.1007/BF00193717

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Keywords

  • Embryo Development
  • Sucrose Concentration
  • Shoot Apical Meristem
  • Root Meristem
  • Shoot Meristem