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Heterogeneities in the distribution of (GACA)n simple repeats in the karyotypes of primates and mouse

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Summary

Tandemly organized simple repetitive sequences are widespread in all eukaryotes. The organization of the simple tetrameric (GACA)n sequences at chromosomal loci has been investigated using in situ hybridization with chemically pure oligonucleotide probes. Both biotin- and digoxigenin-attached (GACA)4 probes reveal specific hybridization signals over the short arms of all acrocentric autosomes in man. In the other examined primates the NOR-bearing autosomes could be detected by in situ hybridization with (GACA)4, and a major concentration of the GACA simple repeats could be observed on the Y chromosome in the gibbon and mouse; the hybridization site in the gibbon Y chromosome coincides particularly with the silver-stainable NOR. In the past, accumulations of (GACA)n sequences were demonstrated mainly on vertebrate sex chromosomes. Therefore, the organization of GACA simple sequences is discussed in the context of their evolutionary potential accumulation and the possible linkage with the primate rDNA loci.

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References

  1. Arnemann J, Jakubiczka S, Schmidtke J, Schäfer R, Epplen JT (1986) Clustered GATA repeats (Bkm sequences) on the human Y chromosome. Hum Genet 73:301–303

  2. Chandra HS (1985) Sex determination: a hypothesis based on non-coding DNA. Proc Natl Acad Sci USA 82:1165–1169

  3. Cheung SW, Sun L, Featherstone T (1989) Visualization of NORs in relation to the precise chromosomal localization of ribosomal RNA genes. Cytogenet Cell Genet 50:93–97

  4. Choo KH, Vissel B, Earle E (1989) Evolution of α-satellite DNA on human acrocentric chromosomes. Genomics 5:332–344

  5. Epplen JT (1988) On simple repeated GATA/GACA sequences in animal genomes: a critical reappraisal. J Hered 79:409–417

  6. Epplen JT, McCarrey JR, Sutou S, Ohno S (1982) Base sequence of a cloned snake W-chromosome DNA fragment and identification of a male-specific putative mRNA in the mouse. Proc Natl Acad Sci USA 79:3798–3802

  7. Epplen JT, Studer R, McLaren A (1988) Heterogeneity in the Sxr(sex-reversal) locus of the mouse as revealed by synthetic GATA/GACA probes. Genet Res 51:239–246

  8. Erickson RP, Ross CE, Gorski JL, Stalvey JRD, Drumm MM (1988) Bkm sequences from the human X chromosome contain large clusters of GATA/GACA repeats. Ann Hum Genet 52:167–176

  9. Evans EP, Breckon G, Ford CE (1964) An air-drying method for meiotic preparations from mammalian testes. Cytogenet Cell Genet 3:289–294

  10. Evans HJ, Buckland RA, Pardue ML (1974) Location of the genes coding for 18 S and 28 S ribosomal RNA in the human genome. Chromosoma 48:405–426

  11. Fowler JCS, Burgoyne LA, Scott AC, Harding HWJ (1988) Repetitive deoxyribonucleic acid (DNA) and human genome variation — a concise review relevant to forensic biology. J Forensic Sci 33:1111–1126

  12. Goodpasture C, Bloom SE (1975) Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma 53:37–50

  13. Hsu TC, Patton JL (1969) Bone marrow preparations for chromosome studies. In: Benirschke K (ed) Comparative mammalian cytogenetics. Springer, Berlin Heidelberg New York, pp 454–460

  14. John B, Miklos GLG (1979) Functional aspects of satellite DNA and heterochromatin. Int Rev Cytol 58:1–114

  15. Jørgensen AL, Bostock CJ, Bak AL (1987) Homologous sub-families of human alphoid repetitive DNA on different nucleolus organizing chromosomes. Proc Natl Acad Sci USA 84:1075–1079

  16. Kiel-Metzger K, Erickson RP (1984) Regional localization of sex-specific Bkm-related sequences on proximal chromosome 17 of mice. Nature 310:579–581

  17. Kiel-Metzger K, Warren G, Wilson GN, Erickson RP (1985) Evidence that the human Y chromosome does not contain clustered DNA sequences (Bkm) associated with heterogametic sex determination in other vertebrates. N Engl J Med 313:242–245

  18. Kurnit DM, Roy S, Stewart GD, Schwedock J, Neve RL, Bruns GAP, Van Keuren ML, Patterson D (1986) The 724 family of DNA sequences is interspersed about the pericentromeric regions of human acrocentric chromosomes. Cytogenet Cell Genet 43:109–116

  19. Lamar EE, Palmer E (1984) Y-encoded, species-specific DNA in mice: evidence that the Y chromosome exists in two poly-morphic forms in inbred strains. Cell 37:171–177

  20. Ledbetter DH (1981) NOR-bearing Y chromosome in a primate, Hylobates (Symphalangus) syndactylus. Cytogenet Cell Genet 29:250–252

  21. Levinson G, Gutman GA (1987) Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Mol Biol Evol 4:203–221

  22. Mikelsaar AV, Schmid M, Krone W, Schwarzacher HG, Schnedl W (1977) Frequency of Ag-stained nucleolus organizer regions in the acrocentric chromosomes of man. Hum Genet 37:73–77

  23. Miklos GLG, Matthaei KI, Reed KC (1989) Occurrence of the (GATA)n sequences in vertebrate and invertebrate genomes. Chromosoma 98:194–200

  24. Nanda I, Epplen JT, Schmid M (1990) In situ hybridisation of non-radioactive oligonucleotide probes to chromosomes. In: Adolph KW (ed) Advanced techniques in chromosome research. Dekker, New York (in press)

  25. Neitzel H (1986) A routine method for the establishment of permanent growing lymphoblastoid cell lines. Hum Genet 73:320–326

  26. Platt THK, Dewey MJ (1987) Multiple forms of male-specific simple repetitive sequences in the genus Mus. J Mol Evol 25:201–206

  27. Schäfer R, Ali S, Epplen JT (1986) The organization of the evolutionarily conserved GATA/GACA repeats in the mouse genome. Chromosoma 93:502–510

  28. Schäfer R, Zischler H, Birsner U, Becker A, Epplen JT (1988) Optimized oligonucleotide probes for DNA fingerprinting. Electrophoresis 9:369–374

  29. Schmid M, Guttenbach M (1988) Evolutionary diversity of reverse (R) fluorescent chromosome bands in vertebrates. Chromosoma 97:101–114

  30. Seuánez HN (1979) The phytogeny of human chromosomes. Springer, Berlin Heidelberg New York

  31. Singh L, Jones KW (1982) Sex reversal in the mouse (Mus musculus) is caused by a recurrent non-reciprocal crossover involving the X and an aberrant Y chromosome. Cell 28:205–216

  32. Singh L, Jones KW (1986) Bkm sequences are polymorphic in humans and are clustered in pericentric regions of various acrocentric chromosomes including the Y. Hum Genet 73:304–308

  33. Singh L, Purdom IF, Jones KW (1981) Conserved sex-chromosome-associated nucleotide sequences in eukaryotes. Cold Spring Harb Symp Quant Biol 45:805–813

  34. Singh L, Phillips C, Jones KW (1984) The conserved nucleotide sequences of Bkm which define Sxr in the mouse are transcribed. Cell 36:111–120

  35. Studer R, Epplen JT (1990) On the organization of animal genomes: ubiquitous interspersion of repetitive DNA sequences. In: Geldermann H, Ellendorff F, Stranzinger G (eds) Proceedings of the Congress on Genome Analysis in Domestic Animals. VCH, Weinheim (in press)

  36. Tantravahi R, Miller DA, Dev VG, Miller OJ (1976) Detection of nucleolus organizer regions in chromosomes of human, chimpanzee, gorilla, orangutan and gibbon. Chromosoma 56:15–27

  37. Tautz D, Renz M (1984) Simple sequences are ubiquitous repetitive components of eukaryotic genomes. Nucleic Acids Res 12:4127–4138

  38. Thein SW, Wallace RB (1986) The use of synthetic oligonucleotides as specific hybridization probes in the diagnosis of genetic disorders. In: Davies KE (ed) Human genetic diseases, a practical approach. IRL Press, Oxford Washington, DC, pp 33–50

  39. Tucker PK, Lee BK, Eicher EM (1989) Y chromosome evolution in the subgenus Mus (genus Mus). Genetics 122:169–179

  40. Varley JM (1977) Patterns of silver staining of human chromosomes. Chromosoma 61:207–214

  41. Waye JS, Willard HF (1989) Human β satellite DNA: genomic organization and sequence definition of a class of highly repetitive tandem DNA. Proc Natl Acad Sci USA 86:6250–6254

  42. Willard HF, Waye JS (1987) Hierarchical order in chromosome-specific human alpha satellite DNA. Trends Genet 3:192–198

  43. Zischler H, Nanda I, Schäfer R, Schmid M, Epplen JT (1989) Digoxigenated oligonucleotide probes specific for simple repeats in DNA fingerprinting and hybridization in situ. Hum Genet 82:227–233

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Correspondence to Michael Schmid.

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Nanda, I., Deubelbeiss, C., Guttenbach, M. et al. Heterogeneities in the distribution of (GACA)n simple repeats in the karyotypes of primates and mouse. Hum Genet 85, 187–194 (1990). https://doi.org/10.1007/BF00193194

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Keywords

  • Biotin
  • Chromosomal Locus
  • Hybridization Signal
  • Repetitive Sequence
  • Oligonucleotide Probe