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Gene transfer into peanut (Arachis hypogaea L.) by Agrobacterium tumefaciens

Abstract

Introduction of foreign genes into plant tissues via Agrobacterium tumefaciens based vectors requires specific knowledge of Agrobacterium-host compatibility. Therefore, to develop a transformation protocol for peanut (Arachis hypogaea L.), five Brazilian cultivars were screened with four wild-type A.tumefaciens strains. Successful transformation was dependent on specific bacterial strain-plant cultivar interactions and strain A281 was the most effective for tumor induction. Tumors displayed hormone autonomous growth, were opine positive and contained DNA that was homologous to the T-DNA of the inciting strain. Tumors induced on seed and seedling explants by A281 (pTD02) also expressed the reporter genes gus and npt-II contained in the binary vector. These results show that peanut is a permissive host for the acceptance of genes from specific A.tumefaciens gene vectors.

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Abbreviations

GUS:

ß-glucuronidase (EC 3.2.1.31)

NPT-II:

neomycin phosphotransferase II (EC 2.7.1.95)

EDTA:

ethylene-diamine-tetracetic acid

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Communicated by I. K. Vasil

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Lacorte, C., Mansur, E., Timmerman, B. et al. Gene transfer into peanut (Arachis hypogaea L.) by Agrobacterium tumefaciens . Plant Cell Reports 10, 354–357 (1991). https://doi.org/10.1007/BF00193158

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Keywords

  • Gene Transfer
  • Reporter Gene
  • Binary Vector
  • Arachis
  • Specific Knowledge