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The site of photoinhibition in leaves of Cucumis sativus L. at low temperatures is photosystem I, not photosystem II

Abstract

Maximum quantum yields (QY) of photosynthetic electron flows through PSI and PSII were separately assessed in thylakoid membranes isolated from leaves of Cucumis sativus L. (cucumber) that had been chilled in various ways. The QY(PSI) in the thylakoids prepared from the leaves treated at 4° C in moderate light at 220 μmol quanta·m−2·s−1 (400–700 nm) for 5 h, was about 20–30% of that in the thylakoids prepared from untreated leaves, while QY(PSII) decreased, at most, by 20% in response to the same treatment. The decrease in QY(PSI) was observed only when the leaves were chilled at temperatures below 10° C, while such a marked temperature dependency was not observed for the decrease in QY(PSII). In the chilling treatment at 4° C for 5 h, the quantum flux density that was required to induce 50% loss of QY (PSI) was ca. 50 umol quanta·m−2·s−1. When the chilling treatment at 4° C in the light was conducted in an atmosphere of N2, photoinhibition of PSI was largely suppressed, while the damage to PSII was somewhat enhanced. The ferricyanide-oxidised minus ascorbate-reduced difference spectra and the light-induced absorbance changes at 700 nm obtained with the thylakoid suspension, indicated the loss of P700 to extents that corresponded to the decreases in QY(PSI). Accordingly, the decreases in QY(PSI) can largely be attributed to destruction of the PSI reaction centre itself. These results clearly show that, at least in cucumber, a typical chillingsensitive plant, PSI is much more susceptible to aerobic photoinhibition than PSII.

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Abbreviations

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

P700:

primary electron donor of PSI

PPFD:

photosynthetically active photon flux density

QY:

quantum yield

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Author information

Correspondence to Kintake Sonoike.

Additional information

We are grateful to invaluable comments by Prof. S. Katoh, K. Hikosaka and the members of our laboratory. We also thank A. Aoyama for technical assistance. This work was partly supported by the grants from the Ministry of Education, Science, and Culture, Japan, to I. Terashima (#03740342 and #04640621).

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Terashima, I., Funayama, S. & Sonoike, K. The site of photoinhibition in leaves of Cucumis sativus L. at low temperatures is photosystem I, not photosystem II. Planta 193, 300–306 (1994). https://doi.org/10.1007/BF00192544

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Key words

  • Chilling stress
  • Cucumis (Cucurbitaceae, cucumber)
  • Photosynthesis
  • Thylakoid
  • Photoinhibition
  • Photosystem I