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Activation of latent DNA-replication origins: a universal effect of cytokinins

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Abstract

In a previous study (Houssa et al., 1990, Planta 181, 324–326) we observed that a single application of a low dose of benzylaminopurine, a cytokinin, resulted in the halving of the size of the units of DNA replication in the vegetative shoot meristem of Sinapis alba L., a dicotyledonous plant. The effect was due to the recruitment by the hormone of latent replication origins in the chromosomal DNA. Here we report that benzylaminopurine has the same effect in both the vegetative shoot meristem of a monocotyledonous species, Lolium temulentum L., and the tomato (Lycopersicon esculentum Mill.) ovule, a dicotyledonous reproductive meristem. It is thus tentatively concluded that activation of latent replication origins is an universal effect of cytokinins in the regulation of the cell-division cycle.

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Abbreviations

BAP:

benzylaminopurine

F:

fork-progression rate

R:

replicon size

TdR:

thymidine

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Author information

Correspondence to Annie Jacqmard.

Additional information

We thank Dr. R.F. Lyndon (Institute of Cell and Molecular Biology, University of Edinburgh, UK) for critical reading of the manuscript. This research was supported by “Region Wallonne” (1861, 2100), “Université de Liège” (Action de Recherche Concertée) and F.R.F.C. (2.4507-87 and 2.4529-92).

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Houssa, C., Bernier, G., Pieltain, A. et al. Activation of latent DNA-replication origins: a universal effect of cytokinins. Planta 193, 247–250 (1994). https://doi.org/10.1007/BF00192537

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Key words

  • Cell division
  • Cytokinin
  • DNA replication
  • Lolium
  • Lycopersicon
  • Replicon
  • Shoot meristem