Changes in the cell membrane glycolipid composition and metabolism are frequently associated with carcinogenesis. The accumulation of globo-series glycolipids is the most notable change of the germ cell glycolipid composition observed in testicular tumours. In this study, the expression of the globo-series core-structure, globotriaosylceramide (Gb3) was investigated in the preinvasive stage of testicular germ cell tumours, carcinoma in situ (CIS). Seventeen tissue specimens with CIS and 12 samples of overt testicular tumours were immunostained with anti-Gb3 monoclonal antibody 38-13. The accumulation of Gb3 was detected in 12 CIS samples (70.6%) and in 8 invasive tumour samples (66.7%), including seminoma, non-seminoma and a combined germ cell tumour. Our findings indicate that the composition of glycolipids shift at the common preinvasive stage of testicular germ cell tumours and confirm that Gb3 is a tumour-associated antigen of testicular germ cell neoplasia.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Andrews PW, Nudelman E, Hakomori S, Fenderson BA (1990) Different patterns of glycolipid antigens are expressed following differentiation of TERA-2 human embryonal carcinoma cells induced by retinoid acid, hexamethylene bis-acetamide (HMBA) or bromodeoxyuridine (BdUR). Differentiation 43:131–138
Damjanov I, Fox N, Knowles BB, Solter D, Lange PH, Fraley EE (1982) Immunohistochemical localization of murine stage-specific embryonic antigen in human testicular germ cell tumors. Am J Pathol 108:225–230
Fenderson BA, Andrews PW, Nudelman E, Clausen H, Hakomori S (1987) Glycolipid core structures switching from globo- to lacto- and ganglio-series during retinoic acid-induced differentiation of TERA-2-derived human embryonal carcinoma cells. Dev Biol 122:21–34
Giwercman A, Cantell L, Marks A (1991) Placental-like alkaline phosphatase as a marker of carcinoma-in-situ of the testis. Comparison with monoclonal antibodies M2A and 43–9F. APMIS 99:586–594
Giwercman A, Andrews PW, Jørgensen N, Müller J, Græm N, Skakkebæk NE (1993) Immunohistochemical expression of embryonal marker TRA-1–60 in carcinoma in situ and germ cell tumors of the testis. Cancer 72:1308–1313
Giwercman A, Lenz S, Skakkebæk NE (1993) Carcinoma in situ in atrophic testis: Biopsy based on abnormal ultrasound pattern. Br J Urol 72:118–120
Hakomori S, Kannagi R (1983) Glycosphingolipids as tumour-associated and differentiation markers. J Natl Cancer Inst 71:231–251
Jacobsen GK, Nørgaard-Pedersen B (1984) Placental alkaline phosphatase in testicular germ cell tumours and in carcinoma-in-situ of the testis. APMIS 92:323–329
Kannagi R, Cochran NA, Ishigami F, Hakomori S, Andrews PW, Knowles BB, Solter D (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globoseries ganglioside isolated from human teratocarcinoma cell. EMBO J 2:2355–2361
Kannagi R, Levery SB, Ishigami F, Hakomori S, Shevinski LH, Knowles BB, Solter D (1983) New globoseries glycosphingolipids in human teratocarcinoma reactive with the monoclonal antibody directed to a developmentally regulated antigen, stage-specific embryonic antigen 3. J Biol Chem 258:8934–8942
Lindholm L, Holmgreen J, Sveenerholm L, Fredman P, Nilsson O, Persson B, Myrvold H, Lagergard T (1983) Monoclonal antibodies against gastrointestinal-tumour-associated antigens isolated as monosialogangliosides. Int Arch Allergy Appl Immunol 71:178–181
Mangeney M, Lingwood CA, Taga S, Caillou B, Tursz T, Wiels J (1993) Apoptosis induced in Burkitt's lymphoma cells via Gb3/CD77, a glycolipid antigen. Cancer Res 53:5314–5319
Nudelman E, Hakomori S, Kannagi R, Levery S, Yen M-Y, Hellstrom I (1982) Characterization of a human melanoma associated ganglioside antigen defined by a monoclonal antibody 4.2. J Biol Chem 257:12752–12756
Ohyama C, Fukushi Y, Satoh M, Saitoh S, Orikasa S, Nudelman E, Straud M, Hakomori S (1990) Changes in glycolipid expression in human testicular tumors. Int J Cancer 45:1040–1044
Ohyama C, Orikasa S, Satoh M, Saito S, Ohtani H, Fukushi Y (1992) Globotriaosyl ceramide glycolipid in seminoma: Its clinicopathological importance in differentiation from testicular malignant lymphoma. J Urol 148:72–75
Rajpert-De Meyts E, Skakkebæk NE (1994) Expression of the c-kit protein product in carcinoma-in-situ and invasive testicular germ cell tumours. Int J Androl 17:85–92
Ruan S, Lloyd KO (1992) Glycosylation pathways in the biosynthesis of gangliosides in melanoma and neuroblastoma cells: Relative glycosyltransferase levels determine ganglioside patterns. Cancer Res 52:5725–5731
Shevinsky LH, Knowles BB, Damjanov I, Solter D (1982) Monoclonal antibody to murine embryos defines a stage-specific embryonic antigen expressed on mouse embryos and human teratocarcinoma cells. Cell 30:697–705
Skakkebæk NE, Berthelsen JG, Giwercman A, Müller J (1987) Carcinoma-in-situ of the testis: Possible origin from gonocytes and precursor of all types of germ cell tumours except spermatocytoma. Int J Androl 10:19–28
Wenk J, Andrews PW, Casper J, Hata J, Pera MF, Keitz A von, Damjanov I, Fenderson BA (1994) Glycolipids of germ cell tumours: Extended globo-series glycolipids are a hallmark of human embryonal carcinoma cells. Int J Cancer 58:108–115
Wiels J, Fellous M, Tursz T (1981) Monoclonal antibody against a Burkitt lymphoma-associated antigen. Proc Natl Acad Sci USA 78:6485–6488
Wiels J, Holmes E, Cochran N, Tursz T, Hakomori S (1984) Enzymatic and organization difference in expression of a Burkitt lymphoma-associated antigen (globotriaosylceramide) in Burkitt lymphoma and lymphoblastoid cell lines. J Biol Chem 259:14783–14787
Wu Z-L, Schwartz E, Seeger R, Ladisch S (1986) Expression of GD2 ganglioside by untreated primary human neuroblastomas. Cancer Res 46:440–443
About this article
Cite this article
Kang, J., Rajpert-De Meyts, E., Skakkebæk, N. et al. Expression of the glycolipid globotriaosylceramide (Gb3) in testicular carcinoma in situ. Vichows Archiv A Pathol Anat 426, 369–374 (1995). https://doi.org/10.1007/BF00191346
- Testicular neoplasms
- Testicular carcinoma in situ (CIS)
- Globotriaosylceramide (Gb3)