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Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 338, Issue 5, pp 510–516 | Cite as

Release of vesicular noradrenaline in the rat tail artery induced by cocaine

  • V. Palaty
Article

Summary

The effects of cocaine on overflows of endogenous noradrenaline and DOPEG from isolated rat tail arteries were examined. 1. Both overflows increased progressively with increasing concentration of cocaine, while the (NA overflow)/(DOPEG overflow) ratio first increased and then decreased. The changes in the overflows induced by cocaine (0.1 mmol/l) appeared reversible. 2. Exposure of the tissue for 30 min to cocaine, 1 mmol/l, resulted in a significant decrease in the proportion of storage vesicles containing electron-dense cores. 3. The changes in overflows of noradrenaline and DOPEG induced by cocaine (0.1 mmol/l) were unaffected by the presence of desipramine (0.1 μmol/l) or removal of extracellular Ca2+. The effect of cocaine on the overflow of noradrenaline was potentiated by prior inhibition of MAO with clorgyline. 4. Exposure of segments to a Ca2+-free, high K, low Na incubation medium was accompanied by increased overflow of noradrenaline. Cocaine (0.1 mmol/l) reduced the overflow of noradrenaline to about a half, and substantially increased the overflow of DOPEG. 5. The increase in the overflow of DOPEG from segments bathed in HEPES-buffered solutions, the pH of which ranged from 6.80 to 7.38, was approximately proportional to the calculated concentration of unprotonated (uncharged) cocaine. 6. Quantitatively similar changes in the overflows were observed when norcocaine was substituted for cocaine. Ecgonine methyl ester was much less potent than cocaine, and O-benzoyl ecgonine was ineffective. 7. The small increases in the overflow of noradrenaline observed at relatively low concentration (<30 μmol/l) of cocaine can be attributed primarily to inhibition of reuptake of the released transmitter by the cocaine- and desipramine-sensitive amine carrier. The overflows of NA and DOPEG in the presence of higher concentrations of the alkaloid exhibit features compatible with the following hypothesis: (A) Cocaine is translocated across the axonal membrane mainly in the form of the unprotonated species, a large fraction of which is reprotonated upon the entry into the axon. (B) Cocaine releases noradrenaline from storage vesicles into the extravesicular space, where the bulk of the amine is converted to DOPEG. (C) Efflux of the remaining noradrenaline from the axon is not mediated by the Na+-dependent, cocaine- and desipramine-sensitive neuronal amine carrier. It seems to represent uncoupled efflux of the protonated form of noradrenaline.

Key words

Noradrenaline Noradrenaline metabolites Cocaine Cocaine metabolites Amine carrier 

Abbreviations

DOPEG

3,4-dihydroxyphenylethylene glycol

DOMA

3,4-dihydroxymandelic acid

HEPES

N-(2-hydroxyethyl)piperazine-N′-ethanesulfonic acid

MAO

monoamine oxidase

MOPEG

3-methoxy-4-hydroxyphenylethylene glycol

NA

(−)noradrenaline

pHj

pH in the extravesicular space of the axon

pHo

pH of the bathing solution

pKa

negative logarithm of the dissociation constant

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • V. Palaty
    • 1
  1. 1.Department of AnatomyThe University of British ColumbiaVancouverCanada

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