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HLA antigens and lymphocytes in proliferative vitreoretinopathy

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Abstract

An immunohistopathological study was performed on 33 epiretinal membrane specimens obtained during vitreoretinal surgery from patients with rhegmatogenous retinal detachment complicated with proliferative vitreoretinopathy (PVR). Immunofluorescence and immunoperoxidase methods were used to detect major histocompatibility complex or HLA antigens and immunocompetent cells, and to identify the cellular and the extracellular component of the proliferative tissue. The following antigens were detected in epiretinal membranes: cytokeratin in 18 (of 18 cases studied for this antigen), glial fibrillary acidic protein in 20 (of 20), vimentin in 20 (of 20), actin in 16 (of 18), fibronectin in 20 (of 20), macrophage CD68 in 16 (of 18), leukocyte common antigen in 12 (of 18), T-cell CD45R in 13 (of 22), HLA-ABC in 27 (of 33), HLA-DR in all the 33 membranes and HLA-DR alpha chain in 32 of 33 membranes. We observed the presence of macrophages and of an activated T-cell population in PVR membranes. HLA-DR antigen expression was found on pigmented and nonpigmented epithelial cells and on mononuclear cells in all epiretinal membranes. The HLA-DR expression on retinal pigmented epithelial cells could play a role in triggering a local immune response in PVR. These findings suggest the involvement of immunological phenomena and probable interactions between the immune system and peptide growth factors.

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References

  1. Bara R, Kusuda M, Caspers-Velu L; Sanui H, Chan C-C, Kuwabara T, Nussenblatt R, GeryI (1989) Cryopexy enhances experimental autoimmune uveoretinitis in rats. Invest Ophthalmol Vis Sci 30:2165–2173

    Google Scholar 

  2. Baudouin Ch, Fredj-Reygrobellet D, Lapalus P, Gastaud P (1988) Immunopathological findings in proliferative diabetic retinopathy. Am J Ophthalmol 105:383–388

    Google Scholar 

  3. Baudouin Ch, Fredj-Reygrobellet D, Baudouin F, Lapalus P, Gastaud P (1989) Immunohistologic study of proliferative vitreoretinopathy. Am J Ophthalmol 108:387–394

    Google Scholar 

  4. Baudouin C, Fredj-Reygrobellet D, Gordon W, Baudouin F, Peyman G, Lapalus Ph, Gastaud P, Bazan N (1990) Class II antigen expression in diabetes preretinal membranes. Am J Ophthalmol 109:70–74

    Google Scholar 

  5. Baudouin C, Gastaud P, Bazan N (1990) Immunohistologic study of epiretinal membranes in proliferative vitreoretinopathy. Am J Ophthalmol 110:593–598

    Google Scholar 

  6. Bottazzo G, Pujol-Burrel R (1986) Organ-specific autoimmunity. An overview. Immunol Rev 94:1370–1467

    Google Scholar 

  7. Brinkman C, Broekhuyse R (1978) Cell-mediated immunity after retinal detachment as determined by lymphocyte stimulation. Am J Ophthalmol 86:260–265

    Google Scholar 

  8. Broekhuyse R, Rademarkers A, Van Vugt A, Winkens H (1990) Autoimmune responsiveness to retinal IRBP, S-antigen and opsin in proliferative vitreoretinopathy. Exp Eye Res 50:197–206

    Google Scholar 

  9. Chan C-C, Detrick B, Nussenblatt R, Palestine A, Fujikawa L, Hook J (1986) HLA-DR antigens on retinal pigment epithelial cells from patients with uveitis. Arch Ophthalmol 104:725–729

    Google Scholar 

  10. Charteris D, Hiscott P, Grierson I, Lightman S (1992) Proliferative vitreoretinopathy. Lymphocytes in epiretinal membranes. Ophthalmology 99:1364–1367

    Google Scholar 

  11. Detrick B, Rodrigues M, Chan C-C, Tso M, Hooks J (1986) Expression of HLA-DR antigen on retinal pigment epithelial cells in retinitis pigmentosa. Am J Ophthalmol 101:584–590

    Google Scholar 

  12. Endres R, Kang A (1988) Induction of la antigens on nonlymphoid cells by lymphokines. Concepts Immunopathol 5:57–59

    Google Scholar 

  13. Foulis A (1986) Class II major histocompatibility complex and organ specific autoimmunity in man. J Pathol 150:5–12

    Google Scholar 

  14. Fujikawa L, Chan C-C, Detrick B, Nussenblatt R (1987) Class II Histocompatibility complex antigens in experimental autoimmune uveitis. Cell Immunol 106:139–145

    Google Scholar 

  15. Gilbert C, Hiscott P, Unger W, Grierson I, Mcleod D (1988) Inflammation and the formation of epiretinal membranes. Eye [Suppl] 2 140–156

    Google Scholar 

  16. Grisanti S, Wiedemann P, Weller M, Heimann K, Zilles K (1991) The significance of complement in proliferative vitreoretinopathy. Invest Ophthalmol Vis Sci 32:2711–2717

    Google Scholar 

  17. Hiscott P, Grierson I, Mcleod D (1984) Retinal pigment epithelial cells in epiretinal membranes: an immunohistochemical study. Br J Ophthalmol 68:708–715

    Google Scholar 

  18. Hitchins C, Grierson I (1988) Intravitreal injection of fibroblasts. The pathological effects on the ocular tissues of the rabbit following an intravitreal injection of autologous skin fibroblasts. Br J Ophthalmol 72:488–492

    Google Scholar 

  19. Hsu S, Raine L, Fangeour H (1982) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedure. J Histochem Cytochem 29:577–580

    Google Scholar 

  20. Hui Y, Sorgente N, Ryan S (1987) Posterior vitreous separation and retinal detachment induced by macrophages. Graefes Arch Clin Exp Ophthalmol 255:279–285

    Google Scholar 

  21. Jerdan J, Pepose J, Michels R, Hayashi R, de Bustros S, Sebag M, Glaser B (1988) Proliferative vitreoretinopathy membranes. An immunohistochemical study. Ophthalmology 96:801–810

    Google Scholar 

  22. Johnson H, Torres B (1985) Peptide growth factors PDGF, EGF and FGF regulate interferon gamma production. J Immunol 134:2824–2831

    Google Scholar 

  23. Kampik A, Kenyon K, Michels R (1981) Epiretinal and vitreous membranes. Comparative study of 56 cases. Arch Ophthalmol 99:1445–1454

    Google Scholar 

  24. Lampson L, Levy R (1980) Two populations of 1a-like molecules on a human B cell line. J Immunol 125:293–299

    Google Scholar 

  25. Proença R, Regadas I, Travassos A (1990) Immunohistopathology of epiretinal membranes. Chibret Int J Ophthalmol 3:47–58

    Google Scholar 

  26. Remy C (1981) Recherche de l'autoimmunité contre la rétine dans les décollements rétiniens idiopathiques. Etude de 50 cas. J Fr Ophthalmol 4:213–216

    Google Scholar 

  27. The Retina Society Terminology Committee (1983) The classification of retinal detachments with proliferative vitreoretinopathy. Ophthalmology 90:121–126

    Google Scholar 

  28. Scheiffarth O, Kampik A, Günther H, Mark von der K (1988) Proteins of the extracellular matrix in vitreoretinal membranes. Graefes Arch Clin Exp Ophthalmol 226:357–361

    Google Scholar 

  29. Scheiffarth O, Tang S, Kampik A (1990) Makrophagen und HLA-DR-Expression bet proliferativer Vitreoretinopathie. Fortschr Ophthalmol 87:340–343

    Google Scholar 

  30. Sramek S, Wallow I, Stevens T, Nork M (1989) Immunostaining of preretinal membranes for actin, fibronectin and glial fibrillary acidic protein. Ophthalmology 96:835–841

    Google Scholar 

  31. Unanue E, Allen P (1987) The basis for the immunoregulatory role of macrophages and other accessory cells. Science 236:551–557

    Google Scholar 

  32. Weller M, Heimann K, Wiedemann P (1988) Immunochemical studies of epiretinal membranes using AAP complexes: evidence for macrophage involvement in traumatic proliferative vitreoretinopathy. Int Ophthalmol 11:181–186

    Google Scholar 

  33. Weller M, Heimann K, Wiedemann P (1988) Demonstration of mononuclear phagocytes in a human epiretinal membrane using a monoclonal antihuman macrophage antibody. Graefes Arch Clin Exp Ophthalmol 226:252–254

    Google Scholar 

  34. Weller M, Wiedemann P, Heimann K, Zilles K (1988) The significance of fibronectin in vitreoretinal pathology: a critical evaluation. Graefes Arch Clin Exp Ophthalmol 226:296–298

    Google Scholar 

  35. Wiedemann P, Heimann K (1986) Proliferative Vitreoretinopathie: Pathogenese und Möglichkeiten der Beeinflussung mit Zytostatika. Klin Monatsbl Augenheilkd 188:559–564

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Centro de Patologia Oftalmica, Hospitais da Universidade de Coimbra, P-3049, Coimbra Codex, Portugal

    Rui Proença, Madalena Carvalho, Didia Proença, Julia Verissimo, Isaura Regadas & Antonio Travassos

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  1. Rui Proença
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  2. Madalena Carvalho
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  3. Didia Proença
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  4. Julia Verissimo
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  5. Isaura Regadas
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  6. Antonio Travassos
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Proença, R., Carvalho, M., Proença, D. et al. HLA antigens and lymphocytes in proliferative vitreoretinopathy. Graefe's Arch Clin Exp Ophthalmol 232, 25–32 (1994). https://doi.org/10.1007/BF00176434

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  • DOI: https://doi.org/10.1007/BF00176434

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