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Assessing post-induction cellular response in a recombinant E. coli lactose-inducible system by monitoring β-galactosidase levels

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Summary

A synthetic lactose-inducible promoter was chosen to study host cell responses to the over-expression of heterologous genes. Fermentations were conducted to compare the effect of induction strategies on the synthesis of β-galactosidase versus the production of recombinant protein. The levels of lactose, IPTG and glucose during induction were manipulated to adjust the utilization of lactose as the inducer and/or the carbon source. In addition, the involvement of the gal operon in lactose metabolism was also explored in order to optimize lactose transport and utilization during induction.

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Authors and Affiliations

  1. Fermentation Process Development, Amgen Inc., 91320-1789, Thousand Oaks, CA, USA

    Clark E. Hartsock III, Ian Leslie, Joseph A. Pope Jr. & Raj Sachdev

  2. Department of Microbiology and Applied Microbial Genetics, Amgen Inc., 91320-1789, Thousand Oaks, CA, USA

    Jeffrey K. Lewis, Larry B. Tsai & Shi-Yuan Meng

Authors
  1. Clark E. Hartsock III
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  2. Jeffrey K. Lewis
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  3. Ian Leslie
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  4. Joseph A. Pope Jr.
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  5. Larry B. Tsai
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  6. Raj Sachdev
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  7. Shi-Yuan Meng
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Hartsock, C.E., Lewis, J.K., Leslie, I. et al. Assessing post-induction cellular response in a recombinant E. coli lactose-inducible system by monitoring β-galactosidase levels. Biotechnol Lett 17, 1025–1030 (1995). https://doi.org/10.1007/BF00143094

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  • DOI: https://doi.org/10.1007/BF00143094

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