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  1. No Access


    Immobilization Techniques of Bacteria for Live Super-resolution Imaging Using Structured Illumination Microscopy

    Advancements in optical microscopy technology have allowed huge progression in the ability to understand protein structure and dynamics in live bacterial cells using fluorescence microscopy. Paramount to high-...

    Amy L. Bottomley, Lynne Turnbull, Cynthia B. Whitchurch in Bacterial Pathogenesis (2017)

  2. No Access


    The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium

    Lectin histochemistry is commonly used to characterize the pattern of glycoconjugates in cells and tissues. Recent studies show that alterations in these glycoconjugates are associated with the entry of cells ...

    Vicente Seco-Rovira, Ester Beltrán-Frutos in Histochemistry of Single Molecules (2017)

  3. No Access


    Detection of Changes in the Medicago sativa Retinoblastoma-Related Protein (MsRBR1) Phosphorylation During Cell Cycle Progression in Synchronized Cell Suspension Culture

    Deepening our knowledge on the regulation of the plant cell division cycle depends on techniques that allow for the enrichment of cell populations in defined cell cycle phases. Synchronization of cell division...

    Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, Gábor V. Horváth in Cell Cycle Synchronization (2017)

  4. No Access


    A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast Cells

    Even though the formation of compact cylindrical chromosomes early during mitosis or meiosis is a prerequisite for the successful segregation of eukaryotic genomes, little is known about the molecular basis of...

    Christoph Schiklenk, Boryana Petrova, Christian H. Haering in Cohesin and Condensin (2017)

  5. No Access


    SH2 Ligand Prediction–Guidance for In-Silico Screening

    Systematic identification of binding partners for SH2 domains is important for understanding the biological function of the corresponding SH2 domain-containing proteins. Here, we describe two different web-acc...

    Shawn S. C. Li, Lei Li in SH2 Domains (2017)

  6. No Access


    Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development

    The Rat Sarcoma (RAS) GTPAse-mediated extracellular signal-regulated kinase (ERK) pathway regulates multiple biological processes across metazoans. In particular during Caenorhabditis elegans oogenesis, ERK signa...

    Jessica Jie Chen, Swathi Arur Ph.D. in ERK Signaling (2017)

  7. No Access


    Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization

    Lipopolysaccharide (LPS) and lipoteichoic acid (LTA) neutralization constitute potential non-antibiotic treatment strategies for sepsis - a systemic infection-induced inflammatory response. Studies on LPS- and...

    Sarah Line Skovbakke, Henrik Franzyk in Antimicrobial Peptides (2017)

  8. No Access


    Molecular Network Dynamics of Cell Cycle Control: Periodicity of Start and Finish

    The cell division cycle is controlled by a complex regulatory network which ensures that the phases of the cell cycle are executed in the right order. This regulatory network receives signals from the environm...

    Alida Palmisano, Judit Zámborszky, Cihan Oguz in Cell Cycle Synchronization (2017)

  9. No Access


    Detection of Senescence Markers During Mammalian Embryonic Development

    Senescence-associated β-galactosidase (SAβ-gal) is a convenient histological technique used to identify senescent cells. Its ease of use is helpful to initially screen and detect senescent cells in heterogeneo...

    Mekayla Storer, William M. Keyes in Oncogene-Induced Senescence (2017)

  10. No Access


    Hapten–Anti-Hapten Technique for Two-Color IHC Detection of Phosphorylated EGFR and H2AX Using Primary Antibodies Raised in the Same Host Species

    Multiplex staining of cell and tissue sections with antibodies raised in the same host species is a serious challenge because of unwanted but inevitable cross-reactivity of secondary antibodies with irrelevant...

    Jodi Hagen, David Schwartz in Signal Transduction Immunohistochemistry (2017)

  11. No Access


    Tools and Strategies for Analysis of Genome-Wide and Gene-Specific DNA Methylation Patterns

    DNA methylation is a stable epigenetic mechanism that has important roles in the normal function of a cell and therefore also in disease etiology. Accurate measurements of normal and altered DNA methylation pa...

    Aniruddha Chatterjee, Euan J. Rodger, Ian M. Morison, Michael R. Eccles in Oral Biology (2017)

  12. No Access


    Protocols for Radiotracer Estimation of Methane Oxidation Rates at In Situ Methane Concentrations in Marine Sediments

    The microbial consumption of methane acts as a biofilter in deep marine environments, significantly limiting the flux of methane from ocean to atmosphere. Measurements of both anaerobic and aerobic oxidation o...

    Ryan Sibert, Vladimir A. Samarkin in Hydrocarbon and Lipid Microbiology Protoco… (2017)

  13. No Access


    Hemolytic Activity of Antimicrobial Peptides

    For antimicrobial peptides to be interesting for systemic applications, they must show low toxicity against erythrocytes. In this chapter, we describe a protocol for measuring the ability of AMPs to lyse human...

    Alberto Oddo, Paul R. Hansen in Antimicrobial Peptides (2017)

  14. No Access


    Protocols for Harvesting a Microbial Community Directly as a Biofilm for the Remediation of Oil Sands Process Water

    The prevalence of inorganic pollutants co-contaminating sites with multiple organic pollutants complicates bioremediation efforts. For this reason, new methods are needed for bioremediation of co-contaminated ...

    Joe Lemire, Marc Demeter, Raymond J. Turner in Hydrocarbon and Lipid Microbiology Protocols (2017)

  15. No Access


    Isolation and Differentiation of Human Macrophages

    Macrophage subtypes display protective or pathogenic activities in vascular lesions. They recognize and engulf modified lipids, accumulate in the arterial intima, contributing to the atherosclerosis developmen...

    Francisco J. Rios Ph.D., Rhian M. Touyz, Augusto C. Montezano in Hypertension (2017)

  16. No Access


    Intracellular Manipulation of Phagosomal Transport and Maturation Using Magnetic Tweezers

    Phagocytosis is an important process of the immune system by which pathogens are internalized and eliminated by phagocytic cells. Upon internalization, the phagosome matures and acidifies while being transport...

    Shashank Shekhar, Vinod Subramaniam, Johannes S. Kanger in Phagocytosis and Phagosomes (2017)

  17. No Access


    Resolving the Genomic Localization of the Kollerin Cohesin-Loader Complex

    The kollerin complex, consisting of Scc2/Scc4 in yeast and Nipbl/Mau2 in vertebrates, is crucial for the chromatin-association of the cohesin complex and therefore for the critical functions of cohesin in cell...

    Kerstin S. Wendt in Cohesin and Condensin (2017)

  18. No Access


    Large-Scale Sequence Comparison

    There are millions of sequences deposited in genomic databases, and it is an important task to categorize them according to their structural and functional roles. Sequence comparison is a prerequisite for prop...

    Devi Lal, Mansi Verma in Bioinformatics (2017)

  19. No Access


    Layer-by-Layer Assembly of Glucose Oxidase on Carbon Nanotube Modified Electrodes

    The use of enzymatically modified electrodes for the detection of glucose or other non-electrochemically active analytes is becoming increasingly common. Direct heterogeneous electron transfer to glucose oxida...

    Alice H. Suroviec in Enzyme Stabilization and Immobilization (2017)

  20. No Access


    Fluorescence-Based Real-Time Activity Assays to Identify RNase P Inhibitors

    Transfer RNA is transcribed as precursor molecules that are processed before participating in translation catalyzed by the ribosome. Ribonuclease P is the endonuclease that catalyzes the 5′ end maturation of p...

    Yu Chen, Xin Liu, Nancy Wu, Carol A. Fierke in Antibiotics (2017)

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