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    Immobilization Techniques of Bacteria for Live Super-resolution Imaging Using Structured Illumination Microscopy

    Advancements in optical microscopy technology have allowed huge progression in the ability to understand protein structure and dynamics in live bacterial cells using fluorescence microscopy. Paramount to high-...

    Amy L. Bottomley, Lynne Turnbull, Cynthia B. Whitchurch in Bacterial Pathogenesis (2017)

  2. No Access

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    Detection of Changes in the Medicago sativa Retinoblastoma-Related Protein (MsRBR1) Phosphorylation During Cell Cycle Progression in Synchronized Cell Suspension Culture

    Deepening our knowledge on the regulation of the plant cell division cycle depends on techniques that allow for the enrichment of cell populations in defined cell cycle phases. Synchronization of cell division...

    Ferhan Ayaydin, Edit Kotogány, Edit Ábrahám, Gábor V. Horváth in Cell Cycle Synchronization (2017)

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    A Protocol for Measuring Mitotic Chromosome Condensation Quantitatively in Fission Yeast Cells

    Even though the formation of compact cylindrical chromosomes early during mitosis or meiosis is a prerequisite for the successful segregation of eukaryotic genomes, little is known about the molecular basis of...

    Christoph Schiklenk, Boryana Petrova, Christian H. Haering in Cohesin and Condensin (2017)

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    SH2 Ligand Prediction–Guidance for In-Silico Screening

    Systematic identification of binding partners for SH2 domains is important for understanding the biological function of the corresponding SH2 domain-containing proteins. Here, we describe two different web-acc...

    Shawn S. C. Li, Lei Li in SH2 Domains (2017)

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    Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development

    The Rat Sarcoma (RAS) GTPAse-mediated extracellular signal-regulated kinase (ERK) pathway regulates multiple biological processes across metazoans. In particular during Caenorhabditis elegans oogenesis, ERK signa...

    Jessica Jie Chen, Swathi Arur Ph.D. in ERK Signaling (2017)

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    Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization

    Lipopolysaccharide (LPS) and lipoteichoic acid (LTA) neutralization constitute potential non-antibiotic treatment strategies for sepsis - a systemic infection-induced inflammatory response. Studies on LPS- and...

    Sarah Line Skovbakke, Henrik Franzyk in Antimicrobial Peptides (2017)

  7. No Access

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    Molecular Network Dynamics of Cell Cycle Control: Periodicity of Start and Finish

    The cell division cycle is controlled by a complex regulatory network which ensures that the phases of the cell cycle are executed in the right order. This regulatory network receives signals from the environm...

    Alida Palmisano, Judit Zámborszky, Cihan Oguz in Cell Cycle Synchronization (2017)

  8. No Access

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    Detection of Senescence Markers During Mammalian Embryonic Development

    Senescence-associated β-galactosidase (SAβ-gal) is a convenient histological technique used to identify senescent cells. Its ease of use is helpful to initially screen and detect senescent cells in heterogeneo...

    Mekayla Storer, William M. Keyes in Oncogene-Induced Senescence (2017)

  9. No Access

    Protocol

    Tools and Strategies for Analysis of Genome-Wide and Gene-Specific DNA Methylation Patterns

    DNA methylation is a stable epigenetic mechanism that has important roles in the normal function of a cell and therefore also in disease etiology. Accurate measurements of normal and altered DNA methylation pa...

    Aniruddha Chatterjee, Euan J. Rodger, Ian M. Morison, Michael R. Eccles in Oral Biology (2017)

  10. No Access

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    Protocols for Radiotracer Estimation of Methane Oxidation Rates at In Situ Methane Concentrations in Marine Sediments

    The microbial consumption of methane acts as a biofilter in deep marine environments, significantly limiting the flux of methane from ocean to atmosphere. Measurements of both anaerobic and aerobic oxidation o...

    Ryan Sibert, Vladimir A. Samarkin in Hydrocarbon and Lipid Microbiology Protoco… (2017)

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    Protocol

    Hemolytic Activity of Antimicrobial Peptides

    For antimicrobial peptides to be interesting for systemic applications, they must show low toxicity against erythrocytes. In this chapter, we describe a protocol for measuring the ability of AMPs to lyse human...

    Alberto Oddo, Paul R. Hansen in Antimicrobial Peptides (2017)

  12. No Access

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    Protocols for Harvesting a Microbial Community Directly as a Biofilm for the Remediation of Oil Sands Process Water

    The prevalence of inorganic pollutants co-contaminating sites with multiple organic pollutants complicates bioremediation efforts. For this reason, new methods are needed for bioremediation of co-contaminated ...

    Joe Lemire, Marc Demeter, Raymond J. Turner in Hydrocarbon and Lipid Microbiology Protocols (2017)

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    Protocol

    Intracellular Manipulation of Phagosomal Transport and Maturation Using Magnetic Tweezers

    Phagocytosis is an important process of the immune system by which pathogens are internalized and eliminated by phagocytic cells. Upon internalization, the phagosome matures and acidifies while being transport...

    Shashank Shekhar, Vinod Subramaniam, Johannes S. Kanger in Phagocytosis and Phagosomes (2017)

  14. No Access

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    Resolving the Genomic Localization of the Kollerin Cohesin-Loader Complex

    The kollerin complex, consisting of Scc2/Scc4 in yeast and Nipbl/Mau2 in vertebrates, is crucial for the chromatin-association of the cohesin complex and therefore for the critical functions of cohesin in cell...

    Kerstin S. Wendt in Cohesin and Condensin (2017)

  15. No Access

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    Large-Scale Sequence Comparison

    There are millions of sequences deposited in genomic databases, and it is an important task to categorize them according to their structural and functional roles. Sequence comparison is a prerequisite for prop...

    Devi Lal, Mansi Verma in Bioinformatics (2017)

  16. No Access

    Protocol

    Layer-by-Layer Assembly of Glucose Oxidase on Carbon Nanotube Modified Electrodes

    The use of enzymatically modified electrodes for the detection of glucose or other non-electrochemically active analytes is becoming increasingly common. Direct heterogeneous electron transfer to glucose oxida...

    Alice H. Suroviec in Enzyme Stabilization and Immobilization (2017)

  17. No Access

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    Fluorescence-Based Real-Time Activity Assays to Identify RNase P Inhibitors

    Transfer RNA is transcribed as precursor molecules that are processed before participating in translation catalyzed by the ribosome. Ribonuclease P is the endonuclease that catalyzes the 5′ end maturation of p...

    Yu Chen, Xin Liu, Nancy Wu, Carol A. Fierke in Antibiotics (2017)

  18. No Access

    Protocol

    Targeting Aflatoxin Biosynthetic Genes

    Chemical detoxification and physical destruction of aflatoxins in foods and feed commodities are mostly unattainable in a way that preserves the edibility of the food. Therefore, preventing mycotoxins in gener...

    Ali Y. Srour, Ahmad M. Fakhoury, Robert L. Brown in Mycotoxigenic Fungi (2017)

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    Protocol

    Protocols for the Study of Microbe–Mineral Interactions in Modern Microbialites

    Microbialites are organo-sedimentary structures formed by the direct or indirect action of microorganisms. Fossil stromatolites (laminated microbialites) constitute the oldest reliable traces of life, a fact t...

    Estelle Couradeau, Karim Benzerara in Hydrocarbon and Lipid Microbiology Protoco… (2017)

  20. No Access

    Protocol

    A Mouse Model for Atopic Dermatitis Using Topical Application of Vitamin D3 or of Its Analog MC903

    Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease with pruritus and high prevalence. Indeed, 15–30 % of children and 2–10 % of adults from industrialized countries are affected. Acute AD ...

    Verena Moosbrugger-Martinz, Matthias Schmuth, Sandrine Dubrac in Inflammation (2017)

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