Synonyms
Triacylglycerols (TAG), phospholipids, cholesteryl esters, or free fatty acids (FFA) are generally not suitable for the GC analysis, and derivatization using short-chain alcohol such as methanol is required. Fatty acid methyl esters (FAME) are preferred because of their greatest volatility and reduced polarity. Alternatively, longer-chain esters have the advantage of providing flame ionization detector (FID) responses that do not require correction factors for their quantification and reduced interferences as well with the solvent peaks and improved positional isomers resolution, although short-chain fatty acids might be lost during aqueous washes (Chouinard et al. 1999; Ulberth et al. 1999). During methylations, O-acyl and N-acyl lipids are transesterified in the presence of a catalyst and excess of methanol that replaces the glycerol or sphingosine moiety. FAME preparation can be performed on lipids isolated from its original matrix or directly on complex...
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References
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Cruz-Hernandez, C., Destaillats, F. (2016). Gas Chromatography of Fatty Acid Methyl Esters: Derivatization. In: Wenk, M. (eds) Encyclopedia of Lipidomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7864-1_66-1
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DOI: https://doi.org/10.1007/978-94-007-7864-1_66-1
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