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Contribution of Yeast Artificial Chromosome-Based Physical Maps to the Final Assembly of the Trypanosoma cruzi Genome

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YAC Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 349))

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Abstract

This chapter describes the methodology used both in performing the electrophoretic karyotype of the protozoan parasite Trypanosoma cruzi and mapping the genetic markers of the chromosomal bands, the construction of chromosome-specific YAC contigs, and their use to assign a chromosomal location to whole genome shotgun sequences.

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References

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Author information

Authors and Affiliations

  1. Department of Microbiology, Immunology and Parasitology, Escola Paulista de Medicina, UNIFESP, S. Paulo, Brazil

    Márcia R. M. Santos, Roberto R. M. Barros, Patrício E. Porcile & José Franco da Silveira

  2. Institute of Genetic Engineering and Molecular Biology, Buenos Aires, Argentina

    Mariano J. Levin

Authors
  1. Márcia R. M. Santos
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  2. Roberto R. M. Barros
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  3. Patrício E. Porcile
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  4. Mariano J. Levin
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  5. José Franco da Silveira
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Editor information

Editors and Affiliations

  1. School of Medical Sciences, College of Life Sciences and Medicine, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, Scotland

    Alasdair MacKenzie

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© 2006 Humana Press Inc., Totowa NJ

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Santos, M.R.M., Barros, R.R.M., Porcile, P.E., Levin, M.J., da Silveira, J.F. (2006). Contribution of Yeast Artificial Chromosome-Based Physical Maps to the Final Assembly of the Trypanosoma cruzi Genome. In: MacKenzie, A. (eds) YAC Protocols. Methods in Molecular Biology™, vol 349. Humana Press. https://doi.org/10.1385/1-59745-158-4:187

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  • DOI: https://doi.org/10.1385/1-59745-158-4:187

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-612-2

  • Online ISBN: 978-1-59745-158-1

  • eBook Packages: Springer Protocols

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