Abstract
We have developed striped metal nanoparticles, Nanobarcodes® particles, which can act as encoded substrates in multiplexed assays. These particles are metallic, encodeable, machine-readable, durable, submicron-sized tags. The power of this technology is that the particles are intrinsically encoded by virtue of the difference in reflectivity of adjacent metal stripes. This chapter describes protocols for the attachment of biological molecules, and the subsequent use of the Nanobarcodes particles in bioassays.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kitano, H. (2002) Systems biology: a brief overview. Science 295, 1662–1664.
Howbrook, D. M., Van der Valk, A. M., O’Shaughnessy, M. C., Sarker, D. K., Baker, S. C., and Lloyd, A. W. (2003) Developments in microarray technologies. Drug Discov. Today 8, 642–651.
Levit-Binnum, N., Lindner, A. B., Zik, O., Eshhar, Z., and Moses, E. (2003) Quantitative detection of protein microarrays. Anal. Chem. 75, 1436–1441.
Nicewarner-Peña, S. R., Freeman, R. G., Reiss, B. D., He, L., Peña, D. J., Walton, I. D., Cromer, R., Keating, C. D., and Natan, M. J. (2001) Submicrometer metallic barcodes. Science 294, 137–141.
Penn, S. G., He, L., and Natan, M. J. (2003) Nanoparticles in bioanalysis. Curr. Opin. Chem. Biol. 7, 609–615.
Nicewarner-Pena, S. R., Carado, A. J., Shale, K. E., and Keating, C. D. (2003) Barcoded metal nanowires: optical reflectivity and patterned fluorescence. J. Phys. Chem. B. 107, 7360–7367.
Walton, I. D., Norton, S. M., Balasingham, A., He, L., Oviso, D. F., Gupta, D., Raju, P. A., Natan, M. J., and Freeman, R. G. (2002) Particles for multiplexed analysis in solution: detection and identification of striped metallic particles using optical microscopy. Anal. Chem. 74, 2240–2247.
Reiss, B. D., Freeman, R. G., Walton, I. D., Norton, S. M., Smith, P. C., Stonas, W. G., Keating, C. D., and Natan, M. J. (2002) Electrochemical synthesis and optical readout of striped metal rods with submicron features. J. Electranal. Chem. 522, 95–103.
Wong, S. S. (1991) Chemistry of Protein Conjugation and Cross-Linking, CRC Press, Boca Raton, FL.
Fox, J. (1997) Applied Regression Analysis, Linear Models, and Related Methods, Sage, Thousand Oaks, CA.
Acknowledgments
We wish to acknowledge the Advanced Technology Program of the National Institute of Standards and Technology for funding (grant no. 70NANB1H3028).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Humana Press Inc.
About this protocol
Cite this protocol
Freeman, R.G. et al. (2005). Use of Nanobarcodes® Particles in Bioassays. In: Rosenthal, S.J., Wright, D.W. (eds) NanoBiotechnology Protocols. Methods in Molecular Biology™, vol 303. Humana Press. https://doi.org/10.1385/1-59259-901-X:073
Download citation
DOI: https://doi.org/10.1385/1-59259-901-X:073
Publisher Name: Humana Press
Print ISBN: 978-1-58829-276-6
Online ISBN: 978-1-59259-901-1
eBook Packages: Springer Protocols