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Peptide-Conjugated Quantum Dots

Imaging the Angiotensin Type 1 Receptor in Living Cells

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NanoBiotechnology Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 303))

Abstract

Peptide-quantum dot conjugates have been prepared by attaching angiotensin II (Ang II) to cadmium selenide/zinc sulfide core-shell nanocrystals using an 1-[3-(Dimethyamino)propyl]-3-ethylcarbo diimide hydrochloride (EDC) coupling. These conjugates have been used to image angiotensin I-expressing Chinese hamster ovary (CHO) cells in vitro. When CHO cells were incubated with Ang II before incubating with Ang II-conjugated quantum dots, we were able to block the binding of the dots. The Ang II-quantum dot conjugates did not bind to parental cells and showed similar staining patterns when compared with a commercially available Ang II Alexa 488™ conjugate.

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Acknowledgments

We wish to thank Quantum Dot for supplying the core-shell nanocrystals used in this study. We also thank Dr. Marcel Bruchez of Quantum Dot for helpful advice during the course of this study. This work was supported by grants from the National Institutes of Health.

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Authors and Affiliations

  1. Department of Chemistry, Vanderbilt University, Nashville, TN

    Ian D. Tomlinson & Sandra J. Rosenthal

  2. Department of Pharmacology, Vanderbilt University School of Medicine, Vanderbilt University, Nashville, TN

    John N. Mason & Randy D. Blakely

Authors
  1. Ian D. Tomlinson
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  2. John N. Mason
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  3. Randy D. Blakely
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  4. Sandra J. Rosenthal
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Editor information

Editors and Affiliations

  1. The Department of Chemistry, Vanderbilt University, Nashville, TN

    Sandra J. Rosenthal  & David W. Wright  & 

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© 2005 Humana Press Inc.

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Tomlinson, I.D., Mason, J.N., Blakely, R.D., Rosenthal, S.J. (2005). Peptide-Conjugated Quantum Dots. In: Rosenthal, S.J., Wright, D.W. (eds) NanoBiotechnology Protocols. Methods in Molecular Biology™, vol 303. Humana Press. https://doi.org/10.1385/1-59259-901-X:051

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  • DOI: https://doi.org/10.1385/1-59259-901-X:051

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-276-6

  • Online ISBN: 978-1-59259-901-1

  • eBook Packages: Springer Protocols

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