Abstract
Lectins array is a powerfull and complementary method of glycans analysis allowing fast identification of specific motifs on molecules or cells. This technology is of increased interest for the development of therapeutic recombinant glycoproteins and particularly relevant for a first study of lot-to-lot comparison, or detection of unwanted glycans. In this chapter, we describe a lectin array-type method specifically designed for the study of recombinant therapeutic interleukin-7 (rhIL-7). This specific method allows the analysis of the glycans motifs, the distribution of the glycoforms population, and the detection of potential immunogen glycans in rhIL-7 purified CHO-produced batches.
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Acknowledgments
This work was carried out with the support of CYTHERIS S.A. The authors acknowledge Dr. Anne Grégoire and Dr. Yann Rancé for their very valuable help and advices.
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Landemarre, L., Duverger, E. (2013). Lectin Glycoprofiling of Recombinant Therapeutic Interleukin-7. In: Beck, A. (eds) Glycosylation Engineering of Biopharmaceuticals. Methods in Molecular Biology, vol 988. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-327-5_14
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DOI: https://doi.org/10.1007/978-1-62703-327-5_14
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Publisher Name: Humana Press, Totowa, NJ
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