Abstract
Purkinje cells of the cerebellar cortex and neurons in the deep cerebellar nuclei (DCN) were among the first central neurons to be studied extensively through the use of in vitro preparations. Yet, the degree to which the patterns of action potential (“spike”) output recorded in these cells in vitro match those recorded in vivo has been a matter of debate and uncertainty. We have identified relatively simple steps that can be applied to the preparation of cerebellar tissue slices or to recording conditions that increase the probability of recording spike output in vitro that more closely matches that found in the live animal. Of particular importance are considerations of the rapid development of Purkinje cell structure and spike output properties during the period used for patch-clamp recordings, reintroducing key synaptic inputs to the in vitro preparation and establishing appropriate reversal potentials for ion species. We also report that storing water to be used for preparing medium in polyethylene carboys introduces a contaminant that decreases the amplitude of T-type Ca2+ currents and the ability to generate rebound burst discharge in DCN cells. Controlling for these factors restores spontaneous tonic firing, increases the gain to parallel fiber input, uncovers bistable rhythmic behavior in Purkinje cells, and increases the probability for generating rebound discharge in DCN cells.
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Acknowledgments
We gratefully acknowledge M. Kruskic and L. Chen for expert technical assistance, G. W. Zamponi for collaborative contributions to the work reviewed here, and D. Jaeger for sharing data for representation. Supported by grants from the Canadian Institute of Health Research (CIHR) to RWT and GWZ and studentship or postdoctoral support through the Alberta Heritage Foundation for Medical Research (AHFMR) (FRF, RT, BEM, JDTE, MI), CIHR (BEM, JDTE), NSERC (BEM), an Achievers in Medical Sciences award (DMA), T. Chen Fong awards (DMA, JDTE), and an Izaak Walton Killam award (DMA). RWT is an AHFMR Scientist.
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McKay, B.E. et al. (2012). Establishing In Vivo-Like Activity in Rat Cerebellar Cells Maintained In Vitro. In: Ballanyi, K. (eds) Isolated Central Nervous System Circuits. Neuromethods, vol 73. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-020-5_7
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