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Neuronal Networks in the In Vitro Isolated Guinea Pig Brain

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Isolated Central Nervous System Circuits

Part of the book series: Neuromethods ((NM,volume 73))

Abstract

Neuronal networks can be properly analyzed in experimental preparations that preserve the largest ­connectivity within and between brain structures. The isolated whole guinea pig brain maintained in vitro by arterial perfusion is ideal for this purpose. The methods and techniques utilized to study brain networks in this preparation are reviewed in this chapter. The advantages, potentials, and limitations for the study of pathological activity in models of acute (and chronic) cerebral diseases, such as epilepsy and ischemia, are also discussed.

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Abbreviations

4AP:

4-aminopyridine

BBB:

Blood-brain barrier

CSD:

Current source density

CNS:

Central nervous system

EC:

Entorhinal cortex

IPSP:

Inhibitory postsynaptic potentials

ID:

Ischemic depolarizations

LOT:

Lateral olfactory tract

MCA:

Medial cerebral artery

OB:

Olfactory bulb

PID:

Peri-infarctual depression

PRC:

Perirhinal cortex

PC:

Piriform cortex

VSD:

Voltage-sensitive dye

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Acknowledgments

Experiments described in this chapter are the result of ∼20 years of research sponsored by the Italian Health Ministry and by grants of the Human Frontiers Science Program Organization, the Mariani Foundation, the CARIPLO Foundation, and the European Community (VSAMUEL and EPICURE).

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Authors and Affiliations

  1. Unit of Experimental Neurophysiology and Epileptology, Fondazione Istituto Neurologico Carlo Besta, Milano, Italy

    Marco de Curtis, Laura Librizzi, Laura Uva & Vadym Gnatkovsky

Authors
  1. Marco de Curtis
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  2. Laura Librizzi
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  3. Laura Uva
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  4. Vadym Gnatkovsky
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Corresponding author

Correspondence to Marco de Curtis .

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Editors and Affiliations

  1. Faculty of Medicine & Dentistry, Department of Physiology and Centre for, University of Alberta, N/A 232D, Edmonton, T6G 2S2, Alberta, Canada

    Klaus Ballanyi

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de Curtis, M., Librizzi, L., Uva, L., Gnatkovsky, V. (2012). Neuronal Networks in the In Vitro Isolated Guinea Pig Brain. In: Ballanyi, K. (eds) Isolated Central Nervous System Circuits. Neuromethods, vol 73. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-020-5_12

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  • DOI: https://doi.org/10.1007/978-1-62703-020-5_12

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