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Construction of Human Naive Antibody Gene Libraries

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Antibody Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 907))

Abstract

Human antibodies are valuable tools for proteome research and diagnostics. Furthermore, antibodies are a rapidly growing class of therapeutic agents, mainly for inflammation and cancer therapy. The first therapeutic antibodies are of murine origin and were chimerized or humanized. The later-developed antibodies are fully human antibodies. Here, two technologies are competing the hybridoma technology using transgenic mice with human antibody gene loci and antibody phage display. The starting point for the selection of human antibodies against any target is the construction of an antibody phage display gene library.

In this review we describe the construction of human naive and immune antibody gene libraries for antibody phage display.

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Acknowledgements

We gratefully acknowledge the financial support by the German ministry of education and research (BMBF, SMP “Antibody Factory” in the NGFN2 program), EU FP6 coordination action ProteomeBinders (contract 026008), and FP7 collaborative projects AffinityProteome (contract 222635) and AFFINOMICS (contract 241481). This review is a combination and updated version of former reviews and book chapters (13, 15, 69).

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Authors and Affiliations

  1. Institut für Biochemie und Biotechnologie, Techische Universität Braunschweig, Braunschweig, Germany

    Michael Hust, André Frenzel, Thomas Schirrmann & Stefan Dübel

  2. Institute für Biochemie and Biotechnologie, Techische Universität Braunschweig, Braunschweig, Germany

    Torsten Meyer

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  1. Michael Hust
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  2. André Frenzel
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  3. Torsten Meyer
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  4. Thomas Schirrmann
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Correspondence to Michael Hust .

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Editors and Affiliations

  1. Antibody Therapeutics & Immunotargeting, INSERM U624, Marseille CEDEX 09, France

    Patrick Chames

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Hust, M., Frenzel, A., Meyer, T., Schirrmann, T., Dübel, S. (2012). Construction of Human Naive Antibody Gene Libraries. In: Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 907. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-974-7_5

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  • DOI: https://doi.org/10.1007/978-1-61779-974-7_5

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