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Antibody Engineering pp 293–302Cite as

Mammalian Cell Surface Display of Full Length IgG

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 907))

Abstract

Display technology has been developed and widely used in antibody screening and selecting. While phage can only display antibody fragments, mammalian cells can display not only fragments but full-length antibodies. Here we described the display of full length antibody on the surface of 293 cells. Both heavy chain and light chain genes were cloned in a single mammalian expression vector containing dual mammalian expression cassettes. While transfected into 293 cells of the vector, both heavy and light chains were expressed. With the help of transmembrane domain of platelet-derived growth factor receptor (PDGFR-TM) fused at the 3′-end of heavy chain in frame, expressed full-length antibodies were displayed on the cell surface and can be easily detected and analyzed by flow cytometry.

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Acknowledgements

This work was supported by funding from an Amgen research grant.

Author information

Authors and Affiliations

  1. Antivirus Research Center, School of Pharmaceutical Science, Southern Medical University, Guangzhou, China

    Chen Zhou

  2. TEVA Pharmaceuticals, Horsham, PA, USA

    Wenyan David Shen

Authors
  1. Chen Zhou
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  2. Wenyan David Shen
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Corresponding author

Correspondence to Wenyan David Shen .

Editor information

Editors and Affiliations

  1. Antibody Therapeutics & Immunotargeting, INSERM U624, Marseille CEDEX 09, France

    Patrick Chames

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© 2012 Springer Science+Business Media, LLC

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Zhou, C., Shen, W.D. (2012). Mammalian Cell Surface Display of Full Length IgG. In: Chames, P. (eds) Antibody Engineering. Methods in Molecular Biology, vol 907. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-974-7_17

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  • DOI: https://doi.org/10.1007/978-1-61779-974-7_17

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-973-0

  • Online ISBN: 978-1-61779-974-7

  • eBook Packages: Springer Protocols

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