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Therapeutic Proteins pp 203–219Cite as

Transient Expression Technologies: Past, Present, and Future

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 899))

Abstract

The first protocols describing transient gene expression in mammalian cells for the rapid generation of recombinant proteins emerged more than 10 years ago as an alternative to the establishment of stable, often amplified clonal cell lines, and relieved somewhat the bias against mammalian cell systems as being too complicated, labor intensive, and tedious to serve as a source for tool proteins in industrial research and academia. Over the past decade, these attempts have been refined and optimized, giving rise to expression protocols applicable in every lab in dependence on available tools, equipment, and envisaged outcome. This chapter summarizes the development of transient expression technologies over the past decade up to its current status and provides an outlook into what may be the future of transient technology development.

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Acknowledgments

We would like to thank our team in the lab for the generation of the experimental data and our colleague Dr. Peter LeMotte, Novartis Cambridge, MA, for critically reviewing the manuscript.

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  1. Novartis Institutes for BioMedical Research, Basel, Switzerland

    Sabine Geisse & Bernd Voedisch

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  1. Sabine Geisse
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  2. Bernd Voedisch
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Correspondence to Sabine Geisse .

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Editors and Affiliations

  1. MedImmune, LLC, MedImmune Way One, Gaithersburg, 20878, USA

    Vladimir Voynov

  2. , Department of Physical Biochemistry, Biogen Idec, Cambridge Center 14, Cambridge, 02142, Massachusetts, USA

    Justin A. Caravella

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Geisse, S., Voedisch, B. (2012). Transient Expression Technologies: Past, Present, and Future. In: Voynov, V., Caravella, J. (eds) Therapeutic Proteins. Methods in Molecular Biology, vol 899. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-921-1_13

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  • DOI: https://doi.org/10.1007/978-1-61779-921-1_13

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