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Cationic Electrophoresis

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Protein Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 869))

Abstract

Denaturing, discontinuous electrophoresis in the presence of SDS has become a standard method for the protein scientist. However, there are situations where this method produces suboptimal results. In these cases, electrophoresis in the presence of positively charged detergents such as cetyltrimethylammonium bromide (CTAB) may work considerably better. Methods for electrophoresis and staining of such gels are presented.

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Acknowledgments

This work was supported in part by Kuwait University grant MPB029 and by Ross University.

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Authors and Affiliations

  1. Ross University School of Medicine, Roseau, Commonwealth of Dominica, West Indies

    Engelbert Buxbaum

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  1. Engelbert Buxbaum
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Correspondence to Engelbert Buxbaum .

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Editors and Affiliations

  1. Oklahoma Medical Research Foundation, Univ. of Oklahoma Health Sciences Center, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, Oklahoma, USA

    R. Hal Scofield

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Buxbaum, E. (2012). Cationic Electrophoresis. In: Kurien, B., Scofield, R. (eds) Protein Electrophoresis. Methods in Molecular Biology, vol 869. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-821-4_6

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  • DOI: https://doi.org/10.1007/978-1-61779-821-4_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-820-7

  • Online ISBN: 978-1-61779-821-4

  • eBook Packages: Springer Protocols

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